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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha : |
19/09/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
DALLA RIZZA, M.; REAL, D.; REYNO, R.; QUESENBERRY, K; BURGUEÑO, J; PORRO, V; ERRICO, E. |
Afiliación : |
MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DANIEL REAL FERREIRO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species |
Fecha de publicación : |
2007 |
Fuente / Imprenta : |
Genetics and Molecular Biology, 2007, v 30, no. 4, p.1118-1124. |
DOI : |
10.1590/S1415-47572007000600015 |
Idioma : |
Inglés |
Notas : |
Article history: Received: March 16, 2007 / Accepted: May 17, 2007.. |
Contenido : |
Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium(89.5%). |
Thesagro : |
ADN; PASTURAS; TRIFOLIUM; VARIABILIDAD GENETICA. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02106naa a2200265 a 4500 001 1052593 005 2019-10-15 008 2007 bl uuuu u00u1 u #d 024 7 $a10.1590/S1415-47572007000600015$2DOI 100 1 $aDALLA RIZZA, M. 245 $aGenetic diversity and DNA content of three South American and three Eurasiatic Trifolium species 260 $c2007 500 $aArticle history: Received: March 16, 2007 / Accepted: May 17, 2007.. 520 $aSix species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium(89.5%). 650 $aADN 650 $aPASTURAS 650 $aTRIFOLIUM 650 $aVARIABILIDAD GENETICA 700 1 $aREAL, D. 700 1 $aREYNO, R. 700 1 $aQUESENBERRY, K 700 1 $aBURGUEÑO, J 700 1 $aPORRO, V 700 1 $aERRICO, E. 773 $tGenetics and Molecular Biology, 2007, v 30, no. 4, p.1118-1124.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
12/03/2021 |
Actualizado : |
03/05/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
BRANCHICCELA, B.; CASTELLI, L.; DIAZ, S.; INVERNIZZI, C; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTUNEZ, K, |
Afiliación : |
MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LORELEY CASTELLI, Departamento de Microbiologia, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo, Uruguay.; SEBASTIAN CARLO DIAZ CETTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CIRO INVERNIZZI, Seccion Etologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay.; YAMANDU MENDOZA SPINA, Seccion Etologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay.; ESTELA SANTOS, Seccion Etologia, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay.; CARLOS JAVIER SILVA VILA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Departamento de Microbiologia, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo, Uruguay.; KARINA ANTUNEZ, Departamento de Microbiologia, Instituto de Investigaciones Biologicas Clemente Estable, Montevideo, Uruguay. |
Título : |
Can pollen supplementation mitigate the impact of nutritional stress on honey bee colonies? |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Journal of Apicultural Research, 2023, Volume 62, Issue 2, pp. 294-302. Doi: https://doi.org/10.1080/00218839.2021.1888537 |
DOI : |
10.1080/00218839.2021.1888537 |
Idioma : |
Inglés |
Notas : |
Article history:Received 27 May 2020/ Accepted 08 Dec 2020/ Published online: 11 Mar 2021.Corresponding author. Email: bbranchiccela@inia.org.uy. This work was supported by the University of the Republic,Uruguay; under grant CSIC C624 and the National Agency of Innovation and Research (ANII); under grant ANII_POS_NAC_2014_1_102247. |
Contenido : |
Abstract:
One of the main driving forces of the Apis mellifera colony losses reported worldwide is the nutritional stress associated with monoculture areas. We previously demonstrated under field conditions that nutritional stress that occurred during the fall impacts honey bee colony strength and health in the short and long-term. Pollen supplementation to colonies during a nutritionally stressful period increased adult and brood populations and decreased the infection level of Nosema ceranae. When brood production continues in the winter season, pollen supplementation during this period could be another strategy to help colonies recover from the negative impact of the nutritional stress that occurred in the fall. Thus, in this study, we analyzed if supplementing colonies with polyfloral pollen in winter could be a useful strategy to mitigate fall nutritional stress. Firstly, colonies were exposed to a natural nutritional stress scenario. Secondly, they were relocated and divided into two experimental groups: one group was supplemented with polyfloral pollen, while the other remained as a control. The strength and health of the colonies were analyzed. Pollen supplementation increased adults and brood populations in the coldest months and it did not affect the infection level of Nosema spp. or RNA viruses. These results suggest that the negative impact of nutritional stress during the fall is difficult to mitigate during the winter. |
Palabras claves : |
BEE HEALTH; NUTRITIONAL STRESS; POLLEN SUPPLEMENTATION. |
Thesagro : |
ABEJAS; APIS MELLIFERA; POLEN. |
Asunto categoría : |
-- |
Marc : |
LEADER 02717naa a2200313 a 4500 001 1061827 005 2023-05-03 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1080/00218839.2021.1888537$2DOI 100 1 $aBRANCHICCELA, B. 245 $aCan pollen supplementation mitigate the impact of nutritional stress on honey bee colonies?$h[electronic resource] 260 $c2023 500 $aArticle history:Received 27 May 2020/ Accepted 08 Dec 2020/ Published online: 11 Mar 2021.Corresponding author. Email: bbranchiccela@inia.org.uy. This work was supported by the University of the Republic,Uruguay; under grant CSIC C624 and the National Agency of Innovation and Research (ANII); under grant ANII_POS_NAC_2014_1_102247. 520 $aAbstract: One of the main driving forces of the Apis mellifera colony losses reported worldwide is the nutritional stress associated with monoculture areas. We previously demonstrated under field conditions that nutritional stress that occurred during the fall impacts honey bee colony strength and health in the short and long-term. Pollen supplementation to colonies during a nutritionally stressful period increased adult and brood populations and decreased the infection level of Nosema ceranae. When brood production continues in the winter season, pollen supplementation during this period could be another strategy to help colonies recover from the negative impact of the nutritional stress that occurred in the fall. Thus, in this study, we analyzed if supplementing colonies with polyfloral pollen in winter could be a useful strategy to mitigate fall nutritional stress. Firstly, colonies were exposed to a natural nutritional stress scenario. Secondly, they were relocated and divided into two experimental groups: one group was supplemented with polyfloral pollen, while the other remained as a control. The strength and health of the colonies were analyzed. Pollen supplementation increased adults and brood populations in the coldest months and it did not affect the infection level of Nosema spp. or RNA viruses. These results suggest that the negative impact of nutritional stress during the fall is difficult to mitigate during the winter. 650 $aABEJAS 650 $aAPIS MELLIFERA 650 $aPOLEN 653 $aBEE HEALTH 653 $aNUTRITIONAL STRESS 653 $aPOLLEN SUPPLEMENTATION 700 1 $aCASTELLI, L. 700 1 $aDIAZ, S. 700 1 $aINVERNIZZI, C 700 1 $aMENDOZA, Y. 700 1 $aSANTOS, E. 700 1 $aSILVA, C. 700 1 $aZUNINO, P. 700 1 $aANTUNEZ, K, 773 $tJournal of Apicultural Research, 2023, Volume 62, Issue 2, pp. 294-302. Doi: https://doi.org/10.1080/00218839.2021.1888537
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