| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
21/02/2014 |
|
Actualizado : |
28/10/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
ORSOMANDO, G.; LORENZI, M.; RAFFAELLI, N.; DALLA RIZZA, M.; MEZZETTI, B.; RUGGIERI, S. |
|
Afiliación : |
GIUSEPPE ORSOMANDO, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; MARIA LORENZI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; NADIA RAFFAELLI, Istituto di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Italy; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; BRUNO MEZZETTI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; SILVERIO RUGGIERI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; Dipto. Biotecnol. Agrarie Ambientali, Università di Ancona, Italy. |
|
Título : |
Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum. |
|
Fecha de publicación : |
2001 |
|
Fuente / Imprenta : |
Journal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584. |
|
ISSN : |
0021-9258 (print) |
|
DOI : |
10.1074/jbc.M101377200 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001.
This work was supported by a grant from the Regione Marche 5b.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This work is dedicated to the memory of Professor Pasquale Rosati.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF354650. |
|
Contenido : |
ABSTRACT.
A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank?/EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ± 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins. |
|
Palabras claves : |
BIODIVERSITY; Fragaria x ananassa; Molecular Sequence Data. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
URL : |
http://www.jbc.org/content/276/24/21578.full.pdf
|
|
Marc : |
LEADER 02675naa a2200253 a 4500
001 1012821
005 2019-10-28
008 2001 bl uuuu u00u1 u #d
022 $a0021-9258 (print)
024 7 $a10.1074/jbc.M101377200$2DOI
100 1 $aORSOMANDO, G.
245 $aPhytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum.$h[electronic resource]
260 $c2001
500 $aArticle history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001. This work was supported by a grant from the Regione Marche 5b. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. This work is dedicated to the memory of Professor Pasquale Rosati. The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF354650.
520 $aABSTRACT. A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank?/EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ± 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins.
653 $aBIODIVERSITY
653 $aFragaria x ananassa
653 $aMolecular Sequence Data
700 1 $aLORENZI, M.
700 1 $aRAFFAELLI, N.
700 1 $aDALLA RIZZA, M.
700 1 $aMEZZETTI, B.
700 1 $aRUGGIERI, S.
773 $tJournal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 133 | |
| 81. |  | MAIDANA, M.; MURCHIO, S.; LEONI, C.; SEÑORALE, M.; DALLA RIZZA, M. Plataformas de expresión funcional del péptido antimicrobiano Aq-AMP2 y su potencial aplicación en patosistemas vegetales. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 40 Financiamiento: Beca de Maestría ANII, INIA Proyecto BT_11 ?Biotecnología de péptidos antimicrobianos.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
|    |
| 82. | | CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 9 p. Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : -- - -- |
| Biblioteca(s): INIA Treinta y Tres. |
| |
| 84. | | ORSOMANDO, G.; LORENZI, M.; RAFFAELLI, N.; DALLA RIZZA, M.; MEZZETTI, B.; RUGGIERI, S. Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum. Journal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584. Article history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001.
This work was supported by a grant from the Regione Marche 5b.
The costs of publication of...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
| Biblioteca(s): INIA Las Brujas. |
| |
| 85. | | ESTEVES, P.; HERNANDEZ, L.; CASTILLO, A.; DALLA RIZZA, M.; QUINCKE, M. Tecnologías para el desarrollo de líneas recombinantes de trigo. MV 20 - COMUNICACIONES LIBRES - MV. MEJORAMIENTO VEGETAL In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 290| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 89. | | SOUZA, A.L.A.; DÍAZ DELLAVALLE, P.; CABRERA, A.; LARRAÑAGA, P.; DALLA RIZZA, M.; DE-SIMONE, S.G. Antimicrobial activity of pleurocidin is retained in Plc-2, a C-terminal 12-amino acid fragment. Peptides, 2013, v.45, p.78-84.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 90. | | LARRAÑAGA, P.; DIAZ, P.; CABRERA, A.; ALEM, D.; ALMEIDA SOUZA, A.L.; GIOVANNI DE SIMONE, S.; DALLA RIZZA, M. Activity of naturally derived antimicrobial peptides against filamentous fungi relevant for agriculture . Sustainable Agriculture Research, 2012, v. 1, no.2, p. 211-221. Article history: Received: June 12, 2012 / Accepted: July 10, 2012 / Online Published: July 23, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : C - 0 |
| Biblioteca(s): INIA Las Brujas. |
| |
| 91. | | REAL, D.; REYNO, R.; DALLA RIZZA, M.; ALTIER, N.; DO CANTO, J.; NARANCIO, R.; FOLLE, G.; BURGUEÑO, J. Caracterización de genotipos de Paspalum notatum por tolerancia a bajas temperaturas y reacción frente a Claviceps paspali ln: Reunión del grupo técnico en forrajeras del Cono Sur, 22., 2008, Minas, Uruguay Bioma campos: innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): INIA; FAO; PROCISUR, 2008. p. 156 Versión impresa y en CD ROM Instituto Nacional de Investigación Agropecuaria, Uruguay; FAO; PROCISUR| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
| |
| 92. | | NAVAJAS, E.; PERAZA, P.; RAVAGNOLO, O.; CIAPPESONI, G.; AGUILAR, I.; KELLY, L.; BRANDA, A.; DALLA RIZZA, M.; MONTOSSI, F. Banco de ADN genómico animal: pilar de una plataforma en selección genómica. Revista INIA Uruguay, 2012, no. 28, p. 20-24 (Revista INIA; 28)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 93. | | BOSCHI, F.; VILARÓ, F.; GALVÁN, G.; SIRI, M. I.; FERREIRA, V.; MURCHIO, S.; SCHVARTZMAN, C.; DALLA RIZZA, M. Aspectos de bioseguridad en la evaluación de papa modificada genéticamente para el control de Ralstonia solanacearum. [p19]. Bloque 2: Mejoramiento por resistencia a enfermedades. In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 4., Jornada Uruguaya de Protección Vegetal, 2., 1° setiembre, 2017, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2017. p. 39.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
| |
| 95. | | REYNO, R.; DALLA RIZZA, M.; CASTILLO, A.; DO CANTO, J.; CONDON, F.; MENESES, L.; LATTANZI, F.; MONZA, J. Estrategias de mejoramiento de forrajeras en Uruguay: enfrentando nuevos desafíos. [Presentación oral]. In: Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; Simposio RedBio Argentina, 11., Montevideo 12-15 noviembre, 2019. Serie Técnica 253: Libro de Resúmenes.| Tipo: Presentaciones Orales |
| Biblioteca(s): INIA Tacuarembó. |
| |
| 97. | | MAIDANA, M.; MURCHIO, S.; SCHVARTZMAN, C.; LEONI, C.; SEÑORALE, M.; MARÍN, M.; REGUERA, M.; BLUMWALD, E.; DALLA RIZZA, M. Herramientas para la expresión de pétptidos antimicrobianos. GGM 37 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 273| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 98. | | CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; GARCIA, C.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. Generación y caracterización de híbridos interespecíficos de L. uliginosus x L. corniculatus ln: Jornada técnica, VI Jornada de agrobiotecnología. INIA Las Brujas, 20 de octubre de 2012 Conocimiento intensivo para el sector productivo: situación actual y perspectivas. Canelones (UY): INIA, 2012. 15-16 (Serie Actividades de Difusión; 698)| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
| |
| 99. | | CASTRO, M.; PETERSON, C.J.; DALLA RIZZA, M.; DÍAZ DELLA VALLE, P.; VÁZQUEZ, D.; IBAÑEZ, W.; ROSS, A. Influence of heat stress on wheat grain characteristics and protein molecular weight distribution. ln: International Wheat Conference, 7., 27 Nov-2 Dec 2005, Mar del Plata, AR Buck, H.T.; Nisi, J.E.; Salomón, N., ed. Wheat production in stressed environments : proceedings. Dordrecht: Springer, 2007. p. 365-371. (Developments in Plant Breeding ; 12)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 100. | | REYNO, R.; JAURENA, M.; REAL, D.; ZARZA, M.; MEROLA, R.; VIANA, A.; DALLA RIZZA, M.; LABANDERA, C. Evaluación de leguminosas forrajeras promisorias en la zona de areniscas ln: Bemhaja, M.; Pittaluga, O., eds. 30 años de investigación en suelos de areniscas, INIA Tacuarembó. Montevideo (Uruguay): INIA, 2006. p. 57-60 (INIA Serie Técnica ; 159)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
| |
| Registros recuperados : 133 | |
|
| Expresión de búsqueda válido. Check! |
|
|
