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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DORSCH, M.; REGIDOR-CERRILLO, J.; ORTEGA-MORA, L.M.; SCIOLI, M.V.; MORRELL, E.L.; CANTÓN, G.J.; MOORE, D.P.; HECKER, Y.P. |
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Afiliación : |
MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay;Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina; JAVIER REGIDOR-CERRILLO, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; LUIS M. ORTEGA-MORA, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; MARÍA V. SCIOLI, María V. Scioli National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; ELEONORA L. MORRELL, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; GERMÁN J. CANTÓN, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; DADÍN P. MOORE, Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina.; YANINA P. HECKER, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina. |
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Título : |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Parasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x |
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DOI : |
10.1007/s00436-020-06922-x |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 12 August 2020 /Accepted: 5 October 2020 |
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Contenido : |
Abstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
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Palabras claves : |
ABORTION; BOVINE; MICROSATELLITE MARKERS; NEOSPORA CANINUM; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
ARGENTINA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02492naa a2200301 a 4500
001 1061592
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s00436-020-06922-x$2DOI
100 1 $aDORSCH, M.
245 $aMicrosatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 12 August 2020 /Accepted: 5 October 2020
520 $aAbstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.
650 $aARGENTINA
653 $aABORTION
653 $aBOVINE
653 $aMICROSATELLITE MARKERS
653 $aNEOSPORA CANINUM
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aREGIDOR-CERRILLO, J.
700 1 $aORTEGA-MORA, L.M.
700 1 $aSCIOLI, M.V.
700 1 $aMORRELL, E.L.
700 1 $aCANTÓN, G.J.
700 1 $aMOORE, D.P.
700 1 $aHECKER, Y.P.
773 $tParasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
26/08/2019 |
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Actualizado : |
29/08/2019 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
DÍAZ-LEYVA, F.A.; HERNÁNDEZ-RODRÍGUEZ, L.; ZAMORA-RODRÍGUEZ, V.; PÉREZ-CASTRO, J.M.; RIVAS, F.; LLANES-ÁLVAREZ, Y.; FERRIOL-MARCHENA, X.; MANZANO-ALFONO, L.; BERTALMIO, A. |
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Afiliación : |
FRANK A. DÍAZ-LEYVA, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; VICTORIA ZAMORA-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; JUANA MARÍA PÉREZ-CASTRO, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; YILIAN LLANES-ÁLVAREZ, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; XENIA FERRIOL-MARCHENA, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; LISET MANZANO-ALFONSO, Instituto de Investigaciones en Fruticultura Tropical, Cuba.; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Detección molecular de viroides de cítricos inoculados en cidro Etrog Arizona 861-S1 cultivada in vitro. [Molecular detection of citrus viroids micrograft-inoculated to Arizona 861-S1 Etrog citron budsticks cultivated in vitro.]. FVO-8. |
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Complemento del título : |
Sección científica: Vigilancia fitosanitaria y manejo de plagas. [Scientific sesion: Phytosanitary surveillance and pest management]. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
In: Instituto de Investigaciones en Fruticultura Tropical (IIFT). Simposio Internacional de Fruticultura Tropical y Subtropical, 5.; Simposio Internacional de Piña, 9., "Fruticultura 2017". Libro de resúmenes."Por una fruticultura competitiva y sostenible". Cuba: Instituto de Investigaciones en Fruticultura Tropical. |
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Páginas : |
p. 8-9 |
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Idioma : |
Español |
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Contenido : |
RESUMEN.
El protocolo para la certificación de viroides del Sistema de Producción de Material de Propagación Certificado de Cítricos de Cuba (SPMPCC), requiere hasta siete meses para obtener los resultados. En este estudio se probó un método para detectar en menor tiempo los viroides CEVd, HSVd y CDVd, mediante la inoculación in vitro de varetas de cidro Etrog Arizona 861-S1 (Citrus medica L.) acoplado a reacciones de RT-PCR.
ABSTRACT.
The minimal time require for viroids certification by the diagnosis protocols of the System of Production of Certified Propagation Material of Citrus of Cuba (SPCPMC) is up to seven months to obtain the results. In this study we tested a method to detect the viroids CEVd, HSVd and CDVd in a shorter time by in vitro inoculation of Arizona 861-S1 Etrog citron (Citrus medica L.) budsticks, coupled to RT-PCR reactions. |
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Palabras claves : |
CDVd-III; CEVd; CVd-IIb; GRAFTING; HSVd; INJERTO; RT-PCR. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13156/1/FVO8.-Rivas-Bertalmio.-2017.-Presentacion-oral.pdf
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Marc : |
LEADER 02126nam a2200301 a 4500
001 1060057
005 2019-08-29
008 2017 bl uuuu u00u1 u #d
100 1 $aDÍAZ-LEYVA, F.A.
245 $aDetección molecular de viroides de cítricos inoculados en cidro Etrog Arizona 861-S1 cultivada in vitro. [Molecular detection of citrus viroids micrograft-inoculated to Arizona 861-S1 Etrog citron budsticks cultivated in vitro.]. FVO-8.$h[electronic resource]
260 $aIn: Instituto de Investigaciones en Fruticultura Tropical (IIFT). Simposio Internacional de Fruticultura Tropical y Subtropical, 5.; Simposio Internacional de Piña, 9., "Fruticultura 2017". Libro de resúmenes."Por una fruticultura competitiva y sostenible". Cuba: Instituto de Investigaciones en Fruticultura Tropical.$c2017
300 $ap. 8-9
520 $aRESUMEN. El protocolo para la certificación de viroides del Sistema de Producción de Material de Propagación Certificado de Cítricos de Cuba (SPMPCC), requiere hasta siete meses para obtener los resultados. En este estudio se probó un método para detectar en menor tiempo los viroides CEVd, HSVd y CDVd, mediante la inoculación in vitro de varetas de cidro Etrog Arizona 861-S1 (Citrus medica L.) acoplado a reacciones de RT-PCR. ABSTRACT. The minimal time require for viroids certification by the diagnosis protocols of the System of Production of Certified Propagation Material of Citrus of Cuba (SPCPMC) is up to seven months to obtain the results. In this study we tested a method to detect the viroids CEVd, HSVd and CDVd in a shorter time by in vitro inoculation of Arizona 861-S1 Etrog citron (Citrus medica L.) budsticks, coupled to RT-PCR reactions.
653 $aCDVd-III
653 $aCEVd
653 $aCVd-IIb
653 $aGRAFTING
653 $aHSVd
653 $aINJERTO
653 $aRT-PCR
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
700 1 $aZAMORA-RODRÍGUEZ, V.
700 1 $aPÉREZ-CASTRO, J.M.
700 1 $aRIVAS, F.
700 1 $aLLANES-ÁLVAREZ, Y.
700 1 $aFERRIOL-MARCHENA, X.
700 1 $aMANZANO-ALFONO, L.
700 1 $aBERTALMIO, A.
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