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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/12/2020 |
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Actualizado : |
11/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VICTOR JULIAN LOPEZ DEL PUERTO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. TAVARES, Forestal Oriental, Paysandú Uruguay.; F. SANTIÑAQUE, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. |
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Complemento del título : |
Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 |
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Páginas : |
9 p. |
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ISSN : |
2301-1548 |
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DOI : |
10.31285/AGRO.24.381 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). |
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Contenido : |
Eucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii. |
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Palabras claves : |
COLCHICINA; COLCHICINE; CULTIVO IN VITRO; EUCALIPTO; IN VITRO CULTURE; ORYZALIN; ORYZALINE; POLIPLOIDIA; POLYPLOIDY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14914/1/Castillo-Agrociencia-2020.pdf
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Marc : |
LEADER 03227naa a2200325 a 4500
001 1061636
005 2021-03-11
008 2020 bl uuuu u00u1 u #d
022 $a2301-1548
024 7 $a10.31285/AGRO.24.381$2DOI
100 1 $aCASTILLO, A.
245 $aPolyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs].$h[electronic resource]
260 $c2020
300 $a9 p.
500 $aArticle history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020).
520 $aEucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii.
653 $aCOLCHICINA
653 $aCOLCHICINE
653 $aCULTIVO IN VITRO
653 $aEUCALIPTO
653 $aIN VITRO CULTURE
653 $aORYZALIN
653 $aORYZALINE
653 $aPOLIPLOIDIA
653 $aPOLYPLOIDY
700 1 $aLOPEZ, V.
700 1 $aTAVARES, E.
700 1 $aSANTIÑAQUE, F.
700 1 $aDALLA RIZZA, M.
773 $tAgrociencia Uruguay 2020$gv. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
29/07/2022 |
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Actualizado : |
31/08/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DORSCH, M.; FRANCIA, M.E.; TANA, L.R.; GONZÁLEZ, F.C.; CABRERA, A.; CALLEROS, L.; SANGUINETTI, M.; BARCELLOS, M.; ZARANTONELLI, L; CIUFFO, C.; MAYA, L.; CASTELLS, M.; MIRAZO, S.; SILVEIRA, C.S.; RABAZA, A.; CAFFARENA, D.; DONCEL, B.; ARÁOZ, V.; MATTO, C.; RMENDANO, J.I.; SALADA, S.; FRAGA, M.; FIERRO, S.; GIANNITTI, F. |
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Afiliación : |
MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Instituto Pasteur de Montevideo, Montevideo, Uruguay.; LEANDRO R. TANA, Laboratorio de Biología de Apicomplejos, Instituto Pasteur de Montevideo, Montevideo, Uruguay.; FABIANA C. GONZÁLEZ, Laboratorio de Biología de Apicomplejos, Instituto Pasteur de Montevideo, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay.; LUCÍA CALLEROS, Sección de Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.; MARGARITA SANGUINETTI, Sección de Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.; MAILA BARCELLOS, Sección de Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.; LETICIA ZARANTONELLI, Unidad Mixta Instituto Pasteur de Montevideo e Instituto Nacional de Investigación Agropecuaria (UMPI), Montevideo, Uruguay.; CAMILA CIUFFO, Unidad Mixta Instituto Pasteur de Montevideo e Instituto Nacional de Investigación Agropecuaria (UMPI), Montevideo, Uruguay.; LETICIA MAYA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay.; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay.; SANTIAGO MIRAZO, Laboratorio de Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA VIRGINIA RABAZA MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Unidad Académica Salud de los Rumiantes, Departamento de Producción Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; BENJAMÍN DONCEL DÍAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratorio de Patología Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá, Bogotá, Colombia.; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA MATTO, Laboratorio Regional Noroeste, División de Laboratorios Veterinarios (DILAVE) Miguel C. Rubino, Ministerio de Ganadería, Agricultura y Pesca (MGAP), Paysandú, Uruguay.; JOAQUÍN I. ARMENDANO, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Tandil, Argentina.; SOFÍA SALADA, Secretariado Uruguayo de la Lana (SUL), Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SERGIO FIERRO, Secretariado Uruguayo de la Lana (SUL), Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diagnostic investigation of 100 cases of abortion in sheep in Uruguay: 2015-2021. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; i. 9:904786. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.904786. |
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DOI : |
10.3389/fvets.2022.904786 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 25 March 2022/Accepted: 13 April 2022/Published: 19 May 2022. |
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Contenido : |
Abstract: The aim of this work was to identify causes of abortion through laboratory investigations in sheep flocks in Uruguay. One hundred cases of abortion, comprising 58 fetuses, 36 fetuses with their placentas, and 6 placentas were investigated in 2015?2021. Cases were subjected to gross and microscopic pathologic examinations, and microbiological and serological testing for the identification of causes of abortion, including protozoal, bacterial, and viral pathogens. An etiologic diagnosis was determined in 46 (46%) cases, including 33 (33%) cases caused by infectious pathogens, as determined by the detection of a pathogen along with the identification of fetoplacental lesions attributable to the detected pathogen. Twenty-seven cases (27%) were caused by Toxoplasma gondii, 5 (5%) by Campylobacter fetus subspecies fetus, and 1 (1%) by an unidentified species of Campylobacter. Fourteen cases (14%) had inflammatory and/or necrotizing fetoplacental lesions compatible with an infectious etiology. Although the cause for these lesions was not clearly identified, T. gondii was detected in 4 of these cases, opportunistic bacteria (Bacillus licheniformis, Streptococcus sp.) were isolated in 2 cases, and bovine viral diarrhea virus 1 subtype i (BVDV-1i) was detected in another. Campylobacter jejuni was identified in 1 (1%) severely autolyzed, mummified fetus. BVDV-2b was identified incidentally in one fetus with an etiologic diagnosis of toxoplasmosis. Microscopic agglutination test revealed antibodies against ?1 Leptospira serovars in 15/63 (23.8%) fetuses; however, Leptospira was not identified by a combination of qPCR, culture, fluorescent antibody testing nor immunohistochemistry. Neospora caninum, Chlamydia abortus, Chlamydia pecorum, Coxiella burnetii and border disease virus were not detected in any of the analyzed cases. Death was attributed to dystocia in 13 (13%) fetuses delivered by 8 sheep, mostly from one highly prolific flock. Congenital malformations including inferior prognathism, a focal hepatic cyst, and enterohepatic agenesis were identified in one fetus each, the latter being the only one considered incompatible with postnatal life. Toxoplasmosis, campylobacteriosis and dystocia were the main identified causes of fetal losses. Despite the relatively low overall success rate in establishing an etiologic diagnosis, a systematic laboratory workup in cases of abortion is of value to identify their causes and enables zoonotic pathogens surveillance. MenosAbstract: The aim of this work was to identify causes of abortion through laboratory investigations in sheep flocks in Uruguay. One hundred cases of abortion, comprising 58 fetuses, 36 fetuses with their placentas, and 6 placentas were investigated in 2015?2021. Cases were subjected to gross and microscopic pathologic examinations, and microbiological and serological testing for the identification of causes of abortion, including protozoal, bacterial, and viral pathogens. An etiologic diagnosis was determined in 46 (46%) cases, including 33 (33%) cases caused by infectious pathogens, as determined by the detection of a pathogen along with the identification of fetoplacental lesions attributable to the detected pathogen. Twenty-seven cases (27%) were caused by Toxoplasma gondii, 5 (5%) by Campylobacter fetus subspecies fetus, and 1 (1%) by an unidentified species of Campylobacter. Fourteen cases (14%) had inflammatory and/or necrotizing fetoplacental lesions compatible with an infectious etiology. Although the cause for these lesions was not clearly identified, T. gondii was detected in 4 of these cases, opportunistic bacteria (Bacillus licheniformis, Streptococcus sp.) were isolated in 2 cases, and bovine viral diarrhea virus 1 subtype i (BVDV-1i) was detected in another. Campylobacter jejuni was identified in 1 (1%) severely autolyzed, mummified fetus. BVDV-2b was identified incidentally in one fetus with an etiologic diagnosis of toxoplasmosis. Microscopic agglutination te... Presentar Todo |
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Palabras claves : |
ABORTION; CAMPYLOBACTEROSIS; DYSTOCIA; INFECTIOUS DISEASES; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; REPRODUCTIVE LOSSES; SHEEP; TOXOPLASMOSIS. |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES; OVEJAS. |
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Asunto categoría : |
L74 Trastornos misceláneos de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16635/1/fvets-09-904786.pdf
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Marc : |
LEADER 04111naa a2200553 a 4500
001 1063471
005 2022-08-31
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fvets.2022.904786$2DOI
100 1 $aDORSCH, M.
245 $aDiagnostic investigation of 100 cases of abortion in sheep in Uruguay$b2015-2021.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 25 March 2022/Accepted: 13 April 2022/Published: 19 May 2022.
520 $aAbstract: The aim of this work was to identify causes of abortion through laboratory investigations in sheep flocks in Uruguay. One hundred cases of abortion, comprising 58 fetuses, 36 fetuses with their placentas, and 6 placentas were investigated in 2015?2021. Cases were subjected to gross and microscopic pathologic examinations, and microbiological and serological testing for the identification of causes of abortion, including protozoal, bacterial, and viral pathogens. An etiologic diagnosis was determined in 46 (46%) cases, including 33 (33%) cases caused by infectious pathogens, as determined by the detection of a pathogen along with the identification of fetoplacental lesions attributable to the detected pathogen. Twenty-seven cases (27%) were caused by Toxoplasma gondii, 5 (5%) by Campylobacter fetus subspecies fetus, and 1 (1%) by an unidentified species of Campylobacter. Fourteen cases (14%) had inflammatory and/or necrotizing fetoplacental lesions compatible with an infectious etiology. Although the cause for these lesions was not clearly identified, T. gondii was detected in 4 of these cases, opportunistic bacteria (Bacillus licheniformis, Streptococcus sp.) were isolated in 2 cases, and bovine viral diarrhea virus 1 subtype i (BVDV-1i) was detected in another. Campylobacter jejuni was identified in 1 (1%) severely autolyzed, mummified fetus. BVDV-2b was identified incidentally in one fetus with an etiologic diagnosis of toxoplasmosis. Microscopic agglutination test revealed antibodies against ?1 Leptospira serovars in 15/63 (23.8%) fetuses; however, Leptospira was not identified by a combination of qPCR, culture, fluorescent antibody testing nor immunohistochemistry. Neospora caninum, Chlamydia abortus, Chlamydia pecorum, Coxiella burnetii and border disease virus were not detected in any of the analyzed cases. Death was attributed to dystocia in 13 (13%) fetuses delivered by 8 sheep, mostly from one highly prolific flock. Congenital malformations including inferior prognathism, a focal hepatic cyst, and enterohepatic agenesis were identified in one fetus each, the latter being the only one considered incompatible with postnatal life. Toxoplasmosis, campylobacteriosis and dystocia were the main identified causes of fetal losses. Despite the relatively low overall success rate in establishing an etiologic diagnosis, a systematic laboratory workup in cases of abortion is of value to identify their causes and enables zoonotic pathogens surveillance.
650 $aENFERMEDADES DE LOS ANIMALES
650 $aOVEJAS
653 $aABORTION
653 $aCAMPYLOBACTEROSIS
653 $aDYSTOCIA
653 $aINFECTIOUS DISEASES
653 $aPATHOLOGY
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aREPRODUCTIVE LOSSES
653 $aSHEEP
653 $aTOXOPLASMOSIS
700 1 $aFRANCIA, M.E.
700 1 $aTANA, L.R.
700 1 $aGONZÁLEZ, F.C.
700 1 $aCABRERA, A.
700 1 $aCALLEROS, L.
700 1 $aSANGUINETTI, M.
700 1 $aBARCELLOS, M.
700 1 $aZARANTONELLI, L
700 1 $aCIUFFO, C.
700 1 $aMAYA, L.
700 1 $aCASTELLS, M.
700 1 $aMIRAZO, S.
700 1 $aSILVEIRA, C.S.
700 1 $aRABAZA, A.
700 1 $aCAFFARENA, D.
700 1 $aDONCEL, B.
700 1 $aARÁOZ, V.
700 1 $aMATTO, C.
700 1 $aRMENDANO, J.I.
700 1 $aSALADA, S.
700 1 $aFRAGA, M.
700 1 $aFIERRO, S.
700 1 $aGIANNITTI, F.
773 $tFrontiers in Veterinary Science, 2022; i. 9:904786. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.904786.
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