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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
18/10/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BOSCHI, F.; SCHVARTZMAN, C.; MURCHIO, S.; FERREIRA, V.; SIRI, M.; GALVÁN, G.; SMOKER, M.; STRANSFEL, L.; ZYPFEL, C.; VILARÓ, F.; DALLA RIZZA, M. |
Afiliación : |
FEDERICO BOSCHI, INASE (Instituto Nacional de Semillas).; CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA FERREIRA, Universidad de la República (UdelaR)/ Facultad de Química; MARÍA SIRI, Universidad de la República (UdelaR)/ Facultad de Química; GUILLERMO GALVÁN, Universidad de la República (UdelaR)/ Facultad de Agronomía; MATTHEW SMOKER, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; LENA STRANSFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; CYRIL ZYPFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Enhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Frontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS. |
DOI : |
10.3389/fpls.2017.01642 |
Idioma : |
Inglés |
Notas : |
THIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management.
Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017. |
Contenido : |
ABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.
© 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza MenosABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterol... Presentar Todo |
Palabras claves : |
BACTERIAL WILT; EFR; PATTERN RECOGNITION RECEPTOR; POTATO; QUANTITATIVE RESISTANCE; RALSTONIA SOLANACEARUM. |
Thesagro : |
PAPA; SOLANUM COMMERSONII; SOLANUM TUBEROSUM. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7375/1/Frontiers-in-Plants-2017-fpls-08-01642.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2017.01642/full
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Marc : |
LEADER 03254naa a2200373 a 4500 001 1057663 005 2019-10-15 008 2017 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2017.01642$2DOI 100 1 $aBOSCHI, F. 245 $aEnhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii.$h[electronic resource] 260 $c2017 500 $aTHIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management. Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017. 520 $aABSTRACT. Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato. © 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza 650 $aPAPA 650 $aSOLANUM COMMERSONII 650 $aSOLANUM TUBEROSUM 653 $aBACTERIAL WILT 653 $aEFR 653 $aPATTERN RECOGNITION RECEPTOR 653 $aPOTATO 653 $aQUANTITATIVE RESISTANCE 653 $aRALSTONIA SOLANACEARUM 700 1 $aSCHVARTZMAN, C. 700 1 $aMURCHIO, S. 700 1 $aFERREIRA, V. 700 1 $aSIRI, M. 700 1 $aGALVÁN, G. 700 1 $aSMOKER, M. 700 1 $aSTRANSFEL, L. 700 1 $aZYPFEL, C. 700 1 $aVILARÓ, F. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
14/07/2015 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Revista INIA |
Autor : |
INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) |
Título : |
Revista INIA Uruguay. (No.10, Marzo 2007). |
Fecha de publicación : |
2007 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2007. |
Páginas : |
48 p. |
Serie : |
(Revista INIA; 10) |
ISSN : |
1510-9011 |
Idioma : |
Español |
Thesagro : |
ARROZ; BIOTECNOLOGIA; BOVINOS DE CARNE; CAMBIO CLIMATICO; CIENCIA; CITRUS; CLIMA; CLIMATOLOGIA; COMUNICACION; CONTROL DE ENFERMEDADES; CULTIVOS DE GRANO; CULTIVOS DE SECANO; ENTOMOLOGIA; ESPECIES FORRAJERAS; EUCALYPTUS; EXPLOTACION AGRICOLA FAMILIAR; FITOPATOLOGÍA; FORESTALES; FORRAJES; FRUTALES; FRUTICULTURA; GANADO BOVINO; GRANOS; GRAS; HORTALIZAS; HORTICULTURA; INIA; INNOVACION; INVESTIGACIÓN; LECHERÍA; LEGUMINOSAS FORRAJERAS; MANEJO DE CULTIVOS; MEJORAMIENTO ANIMAL; METEOROLOGIA; MICROBIOLOGÍA; OVINOS; PASTURAS; PRODUCCIÓN ANIMAL; PRODUCCION DE LANA; PRODUCCION DE LECHE; PRODUCCION LECHERA; REVISTA INIA 2007; SEMILLAS; SOJA; SUELOS; SUINOS; SUSTENTABILIDAD AMBIENTAL; TECNOLOGÍA; TRANSFERENCIA DE TECNOLOGIA; VARIEDADES; VITICULTURA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4820/1/Revista-INIA-10.pdf
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Marc : |
LEADER 01903nam a2200745 a 4500 001 1053055 005 2019-10-15 008 2007 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) 245 $aRevista INIA Uruguay. (No.10, Marzo 2007). 260 $aMontevideo (Uruguay): INIA$c2007 300 $a48 p. 490 $a(Revista INIA; 10) 650 $aARROZ 650 $aBIOTECNOLOGIA 650 $aBOVINOS DE CARNE 650 $aCAMBIO CLIMATICO 650 $aCIENCIA 650 $aCITRUS 650 $aCLIMA 650 $aCLIMATOLOGIA 650 $aCOMUNICACION 650 $aCONTROL DE ENFERMEDADES 650 $aCULTIVOS DE GRANO 650 $aCULTIVOS DE SECANO 650 $aENTOMOLOGIA 650 $aESPECIES FORRAJERAS 650 $aEUCALYPTUS 650 $aEXPLOTACION AGRICOLA FAMILIAR 650 $aFITOPATOLOGÍA 650 $aFORESTALES 650 $aFORRAJES 650 $aFRUTALES 650 $aFRUTICULTURA 650 $aGANADO BOVINO 650 $aGRANOS 650 $aGRAS 650 $aHORTALIZAS 650 $aHORTICULTURA 650 $aINIA 650 $aINNOVACION 650 $aINVESTIGACIÓN 650 $aLECHERÍA 650 $aLEGUMINOSAS FORRAJERAS 650 $aMANEJO DE CULTIVOS 650 $aMEJORAMIENTO ANIMAL 650 $aMETEOROLOGIA 650 $aMICROBIOLOGÍA 650 $aOVINOS 650 $aPASTURAS 650 $aPRODUCCIÓN ANIMAL 650 $aPRODUCCION DE LANA 650 $aPRODUCCION DE LECHE 650 $aPRODUCCION LECHERA 650 $aREVISTA INIA 2007 650 $aSEMILLAS 650 $aSOJA 650 $aSUELOS 650 $aSUINOS 650 $aSUSTENTABILIDAD AMBIENTAL 650 $aTECNOLOGÍA 650 $aTRANSFERENCIA DE TECNOLOGIA 650 $aVARIEDADES 650 $aVITICULTURA
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