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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
19/09/2014 |
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Actualizado : |
23/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RODRIGO TABARE ZARZA FUENTES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. |
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ISSN : |
0304-5609 |
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DOI : |
10.4067/S0718-16202011000300015 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received September 7 2010. /Accepted June 21, 2011. |
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Contenido : |
Abstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. MenosAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in b... Presentar Todo |
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Palabras claves : |
GENETIC VARIABILITY; LOTUS JAPONICUS; MICROSATELLITES; SIMPLE SEQUENCE REPEATS; SSR; VARIABILIDAD GENÉTICA. |
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Thesagro : |
CULTIVOS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.scielo.cl/pdf/ciagr/v38n3/art15.pdf
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Marc : |
LEADER 02470naa a2200301 a 4500
001 1050403
005 2019-10-23
008 2011 bl uuuu u00u1 u #d
022 $a0304-5609
024 7 $a10.4067/S0718-16202011000300015$2DOI
100 1 $aALEM, D.
245 $aMolecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers.$h[electronic resource]
260 $c2011
500 $aArticle history: Received September 7 2010. /Accepted June 21, 2011.
520 $aAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.
650 $aCULTIVOS
653 $aGENETIC VARIABILITY
653 $aLOTUS JAPONICUS
653 $aMICROSATELLITES
653 $aSIMPLE SEQUENCE REPEATS
653 $aSSR
653 $aVARIABILIDAD GENÉTICA
700 1 $aNARANCIO, R.
700 1 $aDIAZ, P.
700 1 $aREBUFFO, M.
700 1 $aZARZA, R.
700 1 $aDALLA RIZZA, M.
773 $tCiencia e Investigación Agraria, Santiago de Chile$gv. 38, n. 3, p. 463-471, 2011.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
19/02/2019 |
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Tipo de producción científica : |
Documentos |
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Autor : |
MONTOSSI, F.; SAN JULIÁN, R.; DE BARBIERI, I.; NOLLA, M.; MEDEROS, A.; LUZARDO, S.; FRANCO, R.; GUTIERREZ, D.; DA SILVA, D.; LEVRATTO, J.; MARTINEZ, H.; BENTANCURT, M.; COSTALES, J. |
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Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN ANDRES NOLLA LAFRANCONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO ANDRES FRANCO AQUINO, Tesista.; DANIEL ANTONIO GUTIERREZ RESTANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DAMIÁN DA SILVA; JUAN CARLOS LEVRATTO CORTES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HOMERO MARTINEZ FORMOSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAURO ANDRES BENTANCURT PONTTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO CESAR COSTALES NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Crecimiento estival de corderos: 2. Alternativas tecnológicas de producción de carne ovina de calidad durante el período estival en la región de Basalto: efecto de la carga animal, sexo y la suplementación con concentrados en corderos pastoreando una pastura de Lotus corniculatus cv. INIA Draco. |
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Fecha de publicación : |
2005 |
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Fuente / Imprenta : |
ln: INIA TACUAREMBÓ. ESTACIÓN EXPERIMENTAL GLENCOE. Día de campo. Producción animal, pasturas y forestal. Estación Experimental Glencoe, Paysandú, octubre, 2005. Tacuarembó (Uruguay): INIA, 2005. |
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Páginas : |
p. 4-7 |
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Serie : |
(INIA Serie Actividades de Difusión ; 431) |
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Idioma : |
Español |
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Contenido : |
Objetivo: Evaluar alternativas tecnológicas para la reducción de la zafralidad de la faena de corderos pesados del Uruguay, utilizando pasturas especializadas para tal fin, en el contexto de los sistemas de producción de la región de Basalto. |
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Palabras claves : |
ANIMAL PRODUCTION; SHEEP. |
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Thesagro : |
CALIDAD; CARNE; CORDERO; DIETA; SUELO BASALTICO; URUGUAY. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10346/1/SAD431p4-7.pdf
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Marc : |
LEADER 01609naa a2200385 a 4500
001 1025583
005 2019-02-19
008 2005 bl uuuu u00u1 u #d
100 1 $aMONTOSSI, F.
245 $aCrecimiento estival de corderos$b2. Alternativas tecnológicas de producción de carne ovina de calidad durante el período estival en la región de Basalto: efecto de la carga animal, sexo y la suplementación con concentrados en corderos pastoreando una pastura de Lotus corniculatus cv. INIA Draco.
260 $c2005
300 $ap. 4-7
490 $a(INIA Serie Actividades de Difusión ; 431)
520 $aObjetivo: Evaluar alternativas tecnológicas para la reducción de la zafralidad de la faena de corderos pesados del Uruguay, utilizando pasturas especializadas para tal fin, en el contexto de los sistemas de producción de la región de Basalto.
650 $aCALIDAD
650 $aCARNE
650 $aCORDERO
650 $aDIETA
650 $aSUELO BASALTICO
650 $aURUGUAY
653 $aANIMAL PRODUCTION
653 $aSHEEP
700 1 $aSAN JULIÁN, R.
700 1 $aDE BARBIERI, I.
700 1 $aNOLLA, M.
700 1 $aMEDEROS, A.
700 1 $aLUZARDO, S.
700 1 $aFRANCO, R.
700 1 $aGUTIERREZ, D.
700 1 $aDA SILVA, D.
700 1 $aLEVRATTO, J.
700 1 $aMARTINEZ, H.
700 1 $aBENTANCURT, M.
700 1 $aCOSTALES, J.
773 $tln: INIA TACUAREMBÓ. ESTACIÓN EXPERIMENTAL GLENCOE. Día de campo. Producción animal, pasturas y forestal. Estación Experimental Glencoe, Paysandú, octubre, 2005. Tacuarembó (Uruguay): INIA, 2005.
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