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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500
001 1051279
005 2019-10-15
008 2014 bl uuuu u00u1 u #d
022 $a1948-5948
024 7 $a10.4172/1948-5948.1000155$2DOI
100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
11/02/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RUBIO, V.; PÉREZ BIDEGAIN, M; BERETTA, A.; BAROLIN, E.; QUINCKE, A. |
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Afiliación : |
VALENTINA RUBIO DELLEPIANE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIO PÉREZ BIDEGAIN, Facultad de la Agronomía, Universidad de la República, Uruguay.; ANDRÉS BERETTA, Manejo y Conservación de Suelos, Ministerio de Ganadería Agricultura y Pesca, Uruguay.; EMILIANO BAROLIN GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN ANDRES QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Impacto de propiedades físico-químicas en la estabilidad estructural de molisoles.(Impact of soil physical and chemical properties on soil aggregate stability of mollisols). |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Revista de la Asociación Argentina de la Ciencia del Suelo (Argentina), volume 37, issue.2, pag. 367-371, 2019.OPEN ACCESS. |
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Idioma : |
Español |
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Notas : |
Article history: Recibido: 5/9/2018 // Recibido con revisiones: 13/5/2019 // Aceptado: 13/5/2019. Autor de contacto: Valentina Rubio (vrubio@inia.org.uy). |
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Contenido : |
RESUMEN:
La estabilidad de agregados (EA) es determinante de la susceptibilidad del suelo a la erosión y compactación.Con el objetivo de evaluar el impacto de las propiedades físico-químicas en la EA de Molisoles, se seleccionaron 16 sitios, de diversa composición físico-química, en los cuales se determinó la EA mediante la técnica de Le
Bissonnais. Un alto porcentaje de la variabilidad de la EA se debió al contenido de arena, Mg y Carbono. Rotaciones que permitan aumentar el COS, ayudarían a prevenir pérdidas en la EA, aunque estos aumentos son de tipo decreciente para dos de los mecanismos de EA evaluados.
ABSTRACT:
Aggregate stability (AS) is a key factor controlling soil erosion risk and compaction. The main objective of this work was to explore the impact of soil physical and chemical properties on AS in Mollisols, using Le Bissonnais method. To achieve this goal, 16 soils were selected to represent a range in soil physicochemical properties. Sand, Mg and organic carbon contents explained a high proportion of variability in AS. Crop rotations that increased soil C would aid in preventing losses in AS, although a decreasing response was observed for two of the
evaluated mechanisms of AS. |
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Palabras claves : |
AGGREGATE STABILITY; CALIDAD FÍSICA DEL SUELO; ESTABILIDAD DE AGREGADOS; LE BISSONNAIS; SOIL PHYSICAL QUALITY. |
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Asunto categoría : |
P33 Química y física del suelo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16715/1/14-Pag-367-371-433.pdf
http://www.suelos.org.ar/publicaciones/Volumen37n2/14-Pag%20367-371%20%23433.pdf
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Marc : |
LEADER 02244naa a2200241 a 4500
001 1060789
005 2022-09-05
008 2019 bl uuuu u00u1 u #d
100 1 $aRUBIO, V.
245 $aImpacto de propiedades físico-químicas en la estabilidad estructural de molisoles.(Impact of soil physical and chemical properties on soil aggregate stability of mollisols).$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 5/9/2018 // Recibido con revisiones: 13/5/2019 // Aceptado: 13/5/2019. Autor de contacto: Valentina Rubio (vrubio@inia.org.uy).
520 $aRESUMEN: La estabilidad de agregados (EA) es determinante de la susceptibilidad del suelo a la erosión y compactación.Con el objetivo de evaluar el impacto de las propiedades físico-químicas en la EA de Molisoles, se seleccionaron 16 sitios, de diversa composición físico-química, en los cuales se determinó la EA mediante la técnica de Le Bissonnais. Un alto porcentaje de la variabilidad de la EA se debió al contenido de arena, Mg y Carbono. Rotaciones que permitan aumentar el COS, ayudarían a prevenir pérdidas en la EA, aunque estos aumentos son de tipo decreciente para dos de los mecanismos de EA evaluados. ABSTRACT: Aggregate stability (AS) is a key factor controlling soil erosion risk and compaction. The main objective of this work was to explore the impact of soil physical and chemical properties on AS in Mollisols, using Le Bissonnais method. To achieve this goal, 16 soils were selected to represent a range in soil physicochemical properties. Sand, Mg and organic carbon contents explained a high proportion of variability in AS. Crop rotations that increased soil C would aid in preventing losses in AS, although a decreasing response was observed for two of the evaluated mechanisms of AS.
653 $aAGGREGATE STABILITY
653 $aCALIDAD FÍSICA DEL SUELO
653 $aESTABILIDAD DE AGREGADOS
653 $aLE BISSONNAIS
653 $aSOIL PHYSICAL QUALITY
700 1 $aPÉREZ BIDEGAIN, M
700 1 $aBERETTA, A.
700 1 $aBAROLIN, E.
700 1 $aQUINCKE, A.
773 $tRevista de la Asociación Argentina de la Ciencia del Suelo (Argentina), volume 37, issue.2, pag. 367-371, 2019.OPEN ACCESS.
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