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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
05/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BAYEMI, P. H.; NSONGKA, V. M.; PERERA, B. M. A. O.; CAVESTANY, D.; WEBB, E. C. |
Afiliación : |
P. H. BAYEMI, Institute of Agricultural Research for Development, Cameroon; V. M. NSONGKA, Institute of Agricultural Research for Development, Cameroon; B. M. A. O. PERERA, University of Peradeniya, Sri Lanka; DANIEL CAVESTANY BOCKING, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. C. WEBB, Department of Animal and Wildlife Sciences, University of Pretoria, South Africa. |
Título : |
Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon. |
Fecha de publicación : |
2007 |
Fuente / Imprenta : |
Tropical Animal Health and Production, June 2007, vol. 39, Issue 5, pages 335-338. |
ISSN : |
0049-4747 // Online ISSN 1573-7438 |
DOI : |
10.1007/s11250-007-9020-6 |
Idioma : |
Inglés |
Notas : |
Article history: Accepted: 8 March 2007 // Published online: 5 June 2007. |
Contenido : |
ABSTRACT.
A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A 450). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ml, with the highest level being 0.75 ng/ml. The mean level was 0.5 ± 0.26 ng/ml. The cows in the 'pregnant group' had progesterone levels ranging from 3.5 to 17.5 ng/ml. This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus. © 2007 Springer Science + Business Media B.V. |
Palabras claves : |
BEEF CATTLE; CAMEROON; CATTLE; ELISA; IMMUNOASSAY; PROGESTERONE; VALIDATION. |
Asunto categoría : |
-- |
Marc : |
LEADER 01891naa a2200289 a 4500 001 1002932 005 2018-12-05 008 2007 bl uuuu u00u1 u #d 022 $a0049-4747 // Online ISSN 1573-7438 024 7 $a10.1007/s11250-007-9020-6$2DOI 100 1 $aBAYEMI, P. H. 245 $aValidation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon.$h[electronic resource] 260 $c2007 500 $aArticle history: Accepted: 8 March 2007 // Published online: 5 June 2007. 520 $aABSTRACT. A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A 450). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ml, with the highest level being 0.75 ng/ml. The mean level was 0.5 ± 0.26 ng/ml. The cows in the 'pregnant group' had progesterone levels ranging from 3.5 to 17.5 ng/ml. This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus. © 2007 Springer Science + Business Media B.V. 653 $aBEEF CATTLE 653 $aCAMEROON 653 $aCATTLE 653 $aELISA 653 $aIMMUNOASSAY 653 $aPROGESTERONE 653 $aVALIDATION 700 1 $aNSONGKA, V. M. 700 1 $aPERERA, B. M. A. O. 700 1 $aCAVESTANY, D. 700 1 $aWEBB, E. C. 773 $tTropical Animal Health and Production, June 2007, vol. 39, Issue 5, pages 335-338.
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