| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
17/04/2024 |
|
Actualizado : |
17/04/2024 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
|
Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
|
Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
|
Complemento del título : |
Technical Article. |
|
Fecha de publicación : |
2023 |
|
Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
|
ISSN : |
2675-9225 |
|
DOI : |
10.4322/2675-9225.00012023 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
|
Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
|
Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
|
Asunto categoría : |
A50 Investigación agraria |
|
URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
|
|
Marc : |
LEADER 02721naa a2200301 a 4500
001 1064596
005 2024-04-17
008 2023 bl uuuu u00u1 u #d
022 $a2675-9225
024 7 $a10.4322/2675-9225.00012023$2DOI
100 1 $aAREVALO, A.P.
245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science.
520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved.
653 $a3R’s
653 $aBiological activity
653 $aErythropoietin
653 $aEuropean Pharmacopeia
653 $aMice
653 $aUNIDAD MIXTA PASTEUR + INIA
700 1 $aBASIKA, T.
700 1 $aANCHETA, S.
700 1 $aPERELMUTER, K.
700 1 $aRICCIARDI, A.
700 1 $aBOLLATI-FOGOLÍN, M.
700 1 $aCRISPO, M.
773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
30/05/2019 |
|
Actualizado : |
10/06/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
ZAGO, D; CANOZZI, M.E.A.; BARCELLOS, J.O,J. |
|
Afiliación : |
Department of Animal Science, Federal University of Rio Grande do Sul. Av. Bento Gonçalves n. 7712, 91540-000, Porto Alegre, RS, Brazil.; MARÍA EUGENIA ANDRIGHETTO CANOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO BARCELLOS, Department of Animal Science, Federal University of Rio Grande do Sul. Av. Bento Gonçalves n. 7712, 91540-000, Porto Alegre, RS, Brazil. |
|
Título : |
Pregnant cow nutrition and its effects on foetal weight-a meta-analysis. |
|
Fecha de publicación : |
2019 |
|
Fuente / Imprenta : |
The Journal of Agricultural Science,Volume 157, Issue 1, January 2019 , pp. 83-95. |
|
DOI : |
10.1017/S0021859619000315 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 5 December 2018//Revised: 5 March 2019//Accepted: 3 April 2019//First published online: 9 May 2019. |
|
Contenido : |
Abstract:The prenatal development of cattle has influence on productive performance throughout postnatal life. The number of muscle and fat cells that the animal will have throughout its life is determined in the foetal stage and is influenced by nutrition of the pregnant cow. A systematic review and meta-analysis was performed to evaluate the effect of different energy levels (total digestible nutrient, TDN) and crude protein (CP) supplied to pregnant cows on foetal weight at 4 (FW4) and 8 months (FW8) and calf birth weight (CBW). Four studies and six trials involving 170 animals were assessed for FW4; four studies, four trials and 156 animals for FW8 and 48 studies, 125 trials and 9053 animals for CBW. High heterogeneity across studies was presented in FW4 (I2 = 94.4%), FW8 (I2 = 91.08%) and CBW (I2 = 96.9%). Dietary TDN and CP levels did not influence FW4. The FW8 was reduced by 2.24 kg when cows were fed 100% of their CP and TDN requirements (I2 = 0%), relative to those fed 70% of their requirements during the first and second trimesters. The CBW was reduced by 0.45 kg (I2 = 96.9%) when cows were fed 130% of their CP requirements relative to other dietary CP levels. When cows were fed 140% of their TDN requirements, CBW decreased by 2.71 kg (I2 = 98.3%) relative to other TDN levels. Dietary energy or CP levels fed above the requirements to pregnant cows restrict foetal development and CBW. |
|
Palabras claves : |
BEEF CATTLE; BIRTH WEIGHT; COW-CALF HERD; FOETAL PROGRAMMING; FOETAL WEIGHT; PESO FETAL; VACA PREÑADA. |
|
Thesagro : |
NUTRICIÓN ANIMAL. |
|
Asunto categoría : |
L51 Fisiología Animal - Nutrición |
|
Marc : |
LEADER 02310naa a2200265 a 4500
001 1059794
005 2019-06-10
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S0021859619000315$2DOI
100 1 $aZAGO, D
245 $aPregnant cow nutrition and its effects on foetal weight-a meta-analysis.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 5 December 2018//Revised: 5 March 2019//Accepted: 3 April 2019//First published online: 9 May 2019.
520 $aAbstract:The prenatal development of cattle has influence on productive performance throughout postnatal life. The number of muscle and fat cells that the animal will have throughout its life is determined in the foetal stage and is influenced by nutrition of the pregnant cow. A systematic review and meta-analysis was performed to evaluate the effect of different energy levels (total digestible nutrient, TDN) and crude protein (CP) supplied to pregnant cows on foetal weight at 4 (FW4) and 8 months (FW8) and calf birth weight (CBW). Four studies and six trials involving 170 animals were assessed for FW4; four studies, four trials and 156 animals for FW8 and 48 studies, 125 trials and 9053 animals for CBW. High heterogeneity across studies was presented in FW4 (I2 = 94.4%), FW8 (I2 = 91.08%) and CBW (I2 = 96.9%). Dietary TDN and CP levels did not influence FW4. The FW8 was reduced by 2.24 kg when cows were fed 100% of their CP and TDN requirements (I2 = 0%), relative to those fed 70% of their requirements during the first and second trimesters. The CBW was reduced by 0.45 kg (I2 = 96.9%) when cows were fed 130% of their CP requirements relative to other dietary CP levels. When cows were fed 140% of their TDN requirements, CBW decreased by 2.71 kg (I2 = 98.3%) relative to other TDN levels. Dietary energy or CP levels fed above the requirements to pregnant cows restrict foetal development and CBW.
650 $aNUTRICIÓN ANIMAL
653 $aBEEF CATTLE
653 $aBIRTH WEIGHT
653 $aCOW-CALF HERD
653 $aFOETAL PROGRAMMING
653 $aFOETAL WEIGHT
653 $aPESO FETAL
653 $aVACA PREÑADA
700 1 $aCANOZZI, M.E.A.
700 1 $aBARCELLOS, J.O,J.
773 $tThe Journal of Agricultural Science,Volume 157, Issue 1, January 2019 , pp. 83-95.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
