| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Tacuarembó. |
|
Fecha : |
11/09/2014 |
|
Actualizado : |
30/09/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
|
Autor : |
BOVE, R.; LÓPEZ, F.; PERERA, C.; CARRACELAS, B.; TORRES, D.; DE SOUZA, G.; AZAMBUJA, C.; BERMÚDEZ, J.; ALZUGARAY, F.; MEDEROS, A. |
|
Afiliación : |
DILAVE.; DILAVE.; DILAVE.; EMERITA BEATRIZ CARRACELAS MARQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO DE SOUZA CAMARGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio Genia, Montevideo, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Diagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus. |
|
Fecha de publicación : |
2013 |
|
Fuente / Imprenta : |
Veterinaria (Montevideo), 2013, v. 49, no. 192, p. 20-28. |
|
Idioma : |
Español |
|
Notas : |
History article: Recibido: 25/02/2013; Aprobado: 01/04/2013. |
|
Contenido : |
En este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis. MenosEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-... Presentar Todo |
|
Palabras claves : |
ABORTION; ABORTO; BOVINE; BOVINOS; CAMPYLOBACTER FETUS VENEREALIS; PCR; REACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE. |
|
Thesagro : |
CAMPYLOBACTER. |
|
Asunto categoría : |
L73 Enfermedades de los animales |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3087/1/CDocuments-and-SettingsachiacchioMis-documentosA-BIBLIOTECA-INIA-TACUAREMBO-TODOARTICULOS-TECNICOS-INIA-EN-REVISTAS-ARBITRADASINIA-TACUAREMBOCARNE-Y-LANAVeterinaria-Montevideo2013v49n192p20-28.pdf
|
|
Marc : |
LEADER 02600naa a2200337 a 4500
001 1050145
005 2019-09-30
008 2013 bl uuuu u00u1 u #d
100 1 $aBOVE, R.
245 $aDiagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus.
260 $c2013
500 $aHistory article: Recibido: 25/02/2013; Aprobado: 01/04/2013.
520 $aEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis.
650 $aCAMPYLOBACTER
653 $aABORTION
653 $aABORTO
653 $aBOVINE
653 $aBOVINOS
653 $aCAMPYLOBACTER FETUS VENEREALIS
653 $aPCR
653 $aREACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE
700 1 $aLÓPEZ, F.
700 1 $aPERERA, C.
700 1 $aCARRACELAS, B.
700 1 $aTORRES, D.
700 1 $aDE SOUZA, G.
700 1 $aAZAMBUJA, C.
700 1 $aBERMÚDEZ, J.
700 1 $aALZUGARAY, F.
700 1 $aMEDEROS, A.
773 $tVeterinaria (Montevideo), 2013$gv. 49, no. 192, p. 20-28.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Tacuarembó (TBO) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
12/12/2017 |
|
Actualizado : |
12/12/2017 |
|
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
|
Autor : |
LADO, B.; BATTENFIELD, S.; SILVA, P.; QUINCKE, M.; GUZMAN, C.; SINGH, R.P.; DREISIGACKER, S.; PEÑA, J.; FRITZ, A.; POLAND, J.; GUTIERREZ, L. |
|
Afiliación : |
BETTINA LADO, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay.; SARAH BATTENFIELD, AgriPro Wheat, Syngenta, 11783 Ascher Rd. Junction City, KS, 66441, USA.; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GUZMAN, CIMMYT, El Batan, Mexico, Mexico.; RAVI P. SINGH, CIMMYT, El Batan, Mexico, Mexico.; SUSANNE DREISIGACKER, CIMMYT, El Batan, Mexico, Mexico.; JAVIER PEÑA, CIMMYT, El Batan, Mexico, Mexico.; ALLAN FRITZ, Wheat Genetics Resource Center, Department of Plant Pathology, 1712 Claflin Rd., Kansas State University, Manhattan, KS 66506, USA; .; JESSE POLAND, Wheat Genetics Resource Center, Department of Plant Pathology, 1712 Claflin Rd., Kansas State University, Manhattan, KS 66506, USA.; LUCIA GUTIERREZ, Department of Agronomy, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706, USA. |
|
Título : |
Comparing strategies to select crosses using genomic prediction in two wheat breeding programs. |
|
Fecha de publicación : |
2017 |
|
Fuente / Imprenta : |
In: International Wheat Genetics Symposium, 12, Tulln, Austria; April 23-28, 2017; BOKU: University of Natural Resources and Life Sciences, Vienna, Austria. |
|
Páginas : |
p.88-90. |
|
Idioma : |
Español |
|
Contenido : |
Key message: Evaluation of crosses prediction methods with and without accounting for progeny variance. Mid-parent values was a much larger factor determining genetic gain than increasing the progeny variance of a cross.
In wheat breeding programs, a critical decision is to determine crosses that have high probability to deliver progenies with higher genetics gains (Zhong & Jannink 2007, Bernardo 2014). We present an application of genomic models for predicting parental cross combinations for grain yield, grain protein, and loaf volume across two wheat-breeding programs, INIA-Uruguay and CIMMYT. We evaluated three methods for selecting the ?best? crosses based on (1) mid-parents, (2) top 10% of the progeny within a cross, and (3) maximizing mean and variance within progeny using thresholds. The last two methods were evaluated with the predicted variances obtained through progeny simulation using the PopVar (Mohammadi et al. 2015, Tiede et al. 2015) package in R software. The first two methods showed 82% of crosses in common for yield, 55% for loaf volume and 53% for grain protein, even though only the second method accounts for the variance of the progeny (Figure 1). While the expected variance of the progeny is important to increase chances of finding superior individuals from transgressive segregation, we observed that the mid-parent values of the crosses selected was a much larger factor determining genetic gain than increasing the progeny variance of a cross (Figure 2). Overall, the genomic resources and the statistical models are now available to plant breeders to predict both the performance of breeding lines per se as well as the value of progeny from any potential crosses, but further understanding on optimizing the cross combinations is needed. MenosKey message: Evaluation of crosses prediction methods with and without accounting for progeny variance. Mid-parent values was a much larger factor determining genetic gain than increasing the progeny variance of a cross.
In wheat breeding programs, a critical decision is to determine crosses that have high probability to deliver progenies with higher genetics gains (Zhong & Jannink 2007, Bernardo 2014). We present an application of genomic models for predicting parental cross combinations for grain yield, grain protein, and loaf volume across two wheat-breeding programs, INIA-Uruguay and CIMMYT. We evaluated three methods for selecting the ?best? crosses based on (1) mid-parents, (2) top 10% of the progeny within a cross, and (3) maximizing mean and variance within progeny using thresholds. The last two methods were evaluated with the predicted variances obtained through progeny simulation using the PopVar (Mohammadi et al. 2015, Tiede et al. 2015) package in R software. The first two methods showed 82% of crosses in common for yield, 55% for loaf volume and 53% for grain protein, even though only the second method accounts for the variance of the progeny (Figure 1). While the expected variance of the progeny is important to increase chances of finding superior individuals from transgressive segregation, we observed that the mid-parent values of the crosses selected was a much larger factor determining genetic gain than increasing the progeny variance of a cross (Figure 2)... Presentar Todo |
|
Palabras claves : |
WHEAT BREEDING PROGRAMS; WHEAT QUALITY. |
|
Thesagro : |
MEJORAMIENTO GENETICO DE PLANTAS; TRIGO. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
Marc : |
LEADER 02689nam a2200289 a 4500
001 1057873
005 2017-12-12
008 2017 bl uuuu u01u1 u #d
100 1 $aLADO, B.
245 $aComparing strategies to select crosses using genomic prediction in two wheat breeding programs.
260 $aIn: International Wheat Genetics Symposium, 12, Tulln, Austria; April 23-28, 2017; BOKU: University of Natural Resources and Life Sciences, Vienna, Austria.$c2017
300 $ap.88-90.
520 $aKey message: Evaluation of crosses prediction methods with and without accounting for progeny variance. Mid-parent values was a much larger factor determining genetic gain than increasing the progeny variance of a cross. In wheat breeding programs, a critical decision is to determine crosses that have high probability to deliver progenies with higher genetics gains (Zhong & Jannink 2007, Bernardo 2014). We present an application of genomic models for predicting parental cross combinations for grain yield, grain protein, and loaf volume across two wheat-breeding programs, INIA-Uruguay and CIMMYT. We evaluated three methods for selecting the ?best? crosses based on (1) mid-parents, (2) top 10% of the progeny within a cross, and (3) maximizing mean and variance within progeny using thresholds. The last two methods were evaluated with the predicted variances obtained through progeny simulation using the PopVar (Mohammadi et al. 2015, Tiede et al. 2015) package in R software. The first two methods showed 82% of crosses in common for yield, 55% for loaf volume and 53% for grain protein, even though only the second method accounts for the variance of the progeny (Figure 1). While the expected variance of the progeny is important to increase chances of finding superior individuals from transgressive segregation, we observed that the mid-parent values of the crosses selected was a much larger factor determining genetic gain than increasing the progeny variance of a cross (Figure 2). Overall, the genomic resources and the statistical models are now available to plant breeders to predict both the performance of breeding lines per se as well as the value of progeny from any potential crosses, but further understanding on optimizing the cross combinations is needed.
650 $aMEJORAMIENTO GENETICO DE PLANTAS
650 $aTRIGO
653 $aWHEAT BREEDING PROGRAMS
653 $aWHEAT QUALITY
700 1 $aBATTENFIELD, S.
700 1 $aSILVA, P.
700 1 $aQUINCKE, M.
700 1 $aGUZMAN, C.
700 1 $aSINGH, R.P.
700 1 $aDREISIGACKER, S.
700 1 $aPEÑA, J.
700 1 $aFRITZ, A.
700 1 $aPOLAND, J.
700 1 $aGUTIERREZ, L.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
