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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha : |
04/02/2020 |
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Actualizado : |
11/08/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SILVEIRA, C.S.; ARMENDANO, J.I.; MOORE, D.P.; CANTÓN, G.J.; MACÍAS-RIOSECO, M.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUÍN IGNACIO ARMENDANO, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Buenos Aires, Argentina; DADÍN PRANDO MOORE, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina; GERMÁN JOSÉ CANTÓN, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Balcarce, Buenos Aires, Argentina; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay]. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Revista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004 |
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ISSN : |
0325-7541 |
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DOI : |
10.1016/j.ram.2019.06.004 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019.
Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud Animal, Estación Experimental La Estanzuela, Colonia, Uruguay; email:carolsilveira7@hotmail.com
Los autores agradecen a los veterinarios que enviaron las muestras de suero e información adicional de los rodeos estudiados al laboratorio de la Plataforma de Investigación en Salud Animal de INIA La Estanzuela. |
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Contenido : |
ABSTRACT.
Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement between these kits, all of them allowed to arrive at similar conclusions regarding the statistical association between N. caninum seropositivity and abortion, thus representing a useful tool for the diagnostic approach at the population level under field conditions.
© 2019 Asociación Argentina de Microbiología
RESUMEN.
A pesar de su importancia, el diagnóstico etiológico del aborto en bovinos representa un desafío técnico para los veterinarios clínicos y laboratoristas, en parte debido a la dificultad de recuperar los fetos abortados en condiciones extensivas de campo. Dicha dificultad limita la posibilidad de detectar tempranamente los vientres abortados y de efectuar la investigación patológica y microbiológica de los abortos. Neospora caninum es un protozoario cosmopolita identificado como uno de los principales agentes abortigénicos en bovinos. En este estudio proponemos una aproximación seroepidemiológica para diagnosticar abortos por N. caninum en bovinos lecheros usando kits de ELISA comerciales. Se procesaron muestras de entre 12 y 93 vacas y/o vaquillonas con (casos) y sin (controles) historia reciente de aborto en 4 tambos comerciales de Uruguay. La proporción controles:casos analizados varió entre 1:1 y 4,6:1. Las muestras (n=230) fueron analizadas mediante 3 kits comerciales para la detección de anticuerpos IgG anti-N. caninum. En los 4 tambos la proporción de vacas y/o vaquillonas seropositivas por cualquier kit fue significativamente mayor en los casos respecto de los controles (odds ratio=5,13 a 36; p=0,0002 a 0,0485). La concordancia entre los kits varió de débil a fuerte (coeficiente Kappa de Cohen=0,58 a 0,83). Concluimos que, a pesar de la imperfecta concordancia entre estos kits, el empleo de todos ellos permitió arribar a conclusiones similares respecto de la asociación estadísticamente significativa entre seropositividad a N. caninum y aborto, lo que demuestra la utilidad de estos kits para la aproximación diagnóstica del aborto a nivel poblacional en condiciones de campo. MenosABSTRACT.
Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement betw... Presentar Todo |
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Palabras claves : |
Cohe?s Kappa coefficient; Neospora caninum; PLATAFORMA SALUD ANIMAL; SEROLOGÍA. |
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Thesagro : |
ABORT; BOVINOS; ELISA; EPIDEMIOLOGIA. |
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Asunto categoría : |
L72 Plagas de los animales |
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URL : |
https://www.elsevier.es/es-revista-revista-argentina-microbiologia-372-pdf-S0325754119300835
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Marc : |
LEADER 05374naa a2200325 a 4500
001 1060678
005 2020-08-11
008 2020 bl uuuu u00u1 u #d
022 $a0325-7541
024 7 $a10.1016/j.ram.2019.06.004$2DOI
100 1 $aSILVEIRA, C.S.
245 $aA comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay].$h[electronic resource]
260 $c2020
500 $aArticle history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019. Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud Animal, Estación Experimental La Estanzuela, Colonia, Uruguay; email:carolsilveira7@hotmail.com Los autores agradecen a los veterinarios que enviaron las muestras de suero e información adicional de los rodeos estudiados al laboratorio de la Plataforma de Investigación en Salud Animal de INIA La Estanzuela.
520 $aABSTRACT. Bovine abortion causes considerable economic losses to the livestock industry worldwide and is of concern for public health and food safety, given that many abortigenic infectious agents of cattle are zoonotic. Despite its importance, the etiological diagnosis of abortion in cattle is challenging both for veterinary practitioners and laboratory technicians, partly due to the difficulty in recovering aborted fetuses under extensive field conditions for pathological and microbiological diagnostic investigation, and in the early identification of aborted dams. Neospora caninum is a cosmopolitan protozoon identified as one of the main abortigenic agents in cattle worldwide. In this study we propose a comparative seroepidemiological approach for the diagnosis of abortion by N. caninum in dairy cattle. Samples from 12 to 93 cows/heifers with and without recent history of abortion (cases and controls) in four commercial dairy farms were tested. The ratio of controls to the cases tested varied from 1:1 to 4.6:1. All samples (n = 230) were analyzed by three commercial ELISA kits for the detection of anti-N. caninum antibodies. In all four dairy farms, the proportion of seropositive cows and/or heifers per kit was significantly higher in the cases than in the controls (Odds Ratios = 5.13 to 36, p = 0.0002 to 0.0485). The agreement among the three kits varied from weak to strong (Cohe?s kappa coefficients = 0.58 to 0.83). We conclude that, despite the imperfect agreement between these kits, all of them allowed to arrive at similar conclusions regarding the statistical association between N. caninum seropositivity and abortion, thus representing a useful tool for the diagnostic approach at the population level under field conditions. © 2019 Asociación Argentina de Microbiología RESUMEN. A pesar de su importancia, el diagnóstico etiológico del aborto en bovinos representa un desafío técnico para los veterinarios clínicos y laboratoristas, en parte debido a la dificultad de recuperar los fetos abortados en condiciones extensivas de campo. Dicha dificultad limita la posibilidad de detectar tempranamente los vientres abortados y de efectuar la investigación patológica y microbiológica de los abortos. Neospora caninum es un protozoario cosmopolita identificado como uno de los principales agentes abortigénicos en bovinos. En este estudio proponemos una aproximación seroepidemiológica para diagnosticar abortos por N. caninum en bovinos lecheros usando kits de ELISA comerciales. Se procesaron muestras de entre 12 y 93 vacas y/o vaquillonas con (casos) y sin (controles) historia reciente de aborto en 4 tambos comerciales de Uruguay. La proporción controles:casos analizados varió entre 1:1 y 4,6:1. Las muestras (n=230) fueron analizadas mediante 3 kits comerciales para la detección de anticuerpos IgG anti-N. caninum. En los 4 tambos la proporción de vacas y/o vaquillonas seropositivas por cualquier kit fue significativamente mayor en los casos respecto de los controles (odds ratio=5,13 a 36; p=0,0002 a 0,0485). La concordancia entre los kits varió de débil a fuerte (coeficiente Kappa de Cohen=0,58 a 0,83). Concluimos que, a pesar de la imperfecta concordancia entre estos kits, el empleo de todos ellos permitió arribar a conclusiones similares respecto de la asociación estadísticamente significativa entre seropositividad a N. caninum y aborto, lo que demuestra la utilidad de estos kits para la aproximación diagnóstica del aborto a nivel poblacional en condiciones de campo.
650 $aABORT
650 $aBOVINOS
650 $aELISA
650 $aEPIDEMIOLOGIA
653 $aCohe?s Kappa coefficient
653 $aNeospora caninum
653 $aPLATAFORMA SALUD ANIMAL
653 $aSEROLOGÍA
700 1 $aARMENDANO, J.I.
700 1 $aMOORE, D.P.
700 1 $aCANTÓN, G.J.
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tRevista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
10/06/2019 |
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Actualizado : |
06/07/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CARRACELAS, G.; HORNBUCKLE, J.; ROSAS, J.E.; ROEL, A. |
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Afiliación : |
JULIO GONZALO CARRACELAS GARRIDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Deakin University, Faculty of Science Engineering & Built Environment, Centre for Regional and Rural Futures, Griffith, New South Wales, Australia.; J. HORNBUCKLE, Deakin University, Faculty of Science Engineering & Built Environment, Centre for Regional and Rural Futures, Griffith, New South Wales, Australia.; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Irrigation management strategies to increase water productivity in Oryza sativa (rice) in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Agricultural Water Management, Volume 222, 1 August 2019, Pages 161-172. DOI: https://doi.org/10.1016/j.agwat.2019.05.049 |
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DOI : |
10.1016/j.agwat.2019.05.049 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 March 2019; Received in revised form 29 May 2019; Accepted 30 May 2019. |
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Contenido : |
Traditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water
depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 1339 kg rice ha−1 (15% reduction) in relation to C. WPi was increased by 0.25 kg m−3 (23%) in IP and 0.68 kg m−3 (62%) in I, in relation to the control C. Whole grain percentage was significantly reduced with I in the North region only. Techniques that maintained the soil water at saturated conditions like intermittent flooding, allowed a reduction of water input with no significant effects
on grain yield, which led to a significant increase in WPi. MenosTraditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water
depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 133... Presentar Todo |
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Palabras claves : |
ALTERNATE WETTING AND DRYING; INTERMITTENT; QUALITY; RICE (ORYZA SATIVA L.); WATER PRODUCTIVITY; YIELD. |
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Thesagro : |
ARROZ. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02901naa a2200265 a 4500
001 1059837
005 2020-07-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.agwat.2019.05.049$2DOI
100 1 $aCARRACELAS, G.
245 $aIrrigation management strategies to increase water productivity in Oryza sativa (rice) in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 29 March 2019; Received in revised form 29 May 2019; Accepted 30 May 2019.
520 $aTraditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 1339 kg rice ha−1 (15% reduction) in relation to C. WPi was increased by 0.25 kg m−3 (23%) in IP and 0.68 kg m−3 (62%) in I, in relation to the control C. Whole grain percentage was significantly reduced with I in the North region only. Techniques that maintained the soil water at saturated conditions like intermittent flooding, allowed a reduction of water input with no significant effects on grain yield, which led to a significant increase in WPi.
650 $aARROZ
653 $aALTERNATE WETTING AND DRYING
653 $aINTERMITTENT
653 $aQUALITY
653 $aRICE (ORYZA SATIVA L.)
653 $aWATER PRODUCTIVITY
653 $aYIELD
700 1 $aHORNBUCKLE, J.
700 1 $aROSAS, J.E.
700 1 $aROEL, A.
773 $tAgricultural Water Management, Volume 222, 1 August 2019, Pages 161-172. DOI: https://doi.org/10.1016/j.agwat.2019.05.049
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