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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/03/2023 |
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Actualizado : |
27/04/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CORONA, M.; BRANCHICCELA, B.; ALBURAKI, M.; PALMER-YOUNG , E.C.; MADELLA , S.; CHEN, Y.; EVANS, J.D. |
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Afiliación : |
MIGUEL CORONA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MOHAMED ALBURAKI, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; EVAN C. PALMER-YOUNG, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; SHAYNE MADELLA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; YANPING CHEN, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; JAY D. EVANS, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States. |
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Título : |
Decoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health. |
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Complemento del título : |
Original Research article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Frontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840 |
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ISSN : |
1664-042X (Online) |
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DOI : |
10.3389/fphys.2023.1149840 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov -- Edited by: Zhiguo Li, Fujian Agriculture and Forestry University, China. -- Reviewed by: Chunsheng Hou, Institute of Bast Fiber Crops (CAAS), China, Petar Hristov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria. -- This article is part of the Research Topic Biotic and Abiotic Stresses on Honeybee Physiology and and Colony Health (https://www.frontiersin.org/research-topics/41726/biotic-and-abiotic-stresses-on-honeybee-physiology-and-colony-health#articles ). -- LICENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (http://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
Nutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the experimental variables on viral titers, including higher Deformed wing virus (DWV) titers associated with foraging and age-related decline. In addition, nutrition significantly affected DWV titers in young nurses, with higher titers induced by pollen ingestion. In contrast, higher levels of Black queen cell virus (BQCV) were associated with pollen restriction. Finally, correlation, PCA, and NMDS analyses proved that behavior had had the strongest effect on gene expression and viral titers, followed by age and nutrition. These analyses also support multiple interactions among genes and virus analyzed, including negative correlations between the expression of genes encoding storage proteins associated with pollen ingestion and nursing (vg and mrjp1) with the expression of immune genes and DWV titers. Our results provide new insights into the proximal mechanisms by which nutritional stress is associated with changes in honey bee physiology, immunity, and viral titers. Copyright © 2023 Corona, Branchiccela, Alburaki, Palmer-Young, Madella, Chen and Evans. MenosNutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the ex... Presentar Todo |
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Palabras claves : |
Dorsal; DWV; Immune genes; Insulin; MFE; MRJP1; Vitellogenin. |
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Thesagro : |
APICULTURA; APIS MELLIFERA. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.frontiersin.org/articles/10.3389/fphys.2023.1149840/pdf
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Marc : |
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022 $a1664-042X (Online)
024 7 $a10.3389/fphys.2023.1149840$2DOI
100 1 $aCORONA, M.
245 $aDecoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov -- Edited by: Zhiguo Li, Fujian Agriculture and Forestry University, China. -- Reviewed by: Chunsheng Hou, Institute of Bast Fiber Crops (CAAS), China, Petar Hristov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria. -- This article is part of the Research Topic Biotic and Abiotic Stresses on Honeybee Physiology and and Colony Health (https://www.frontiersin.org/research-topics/41726/biotic-and-abiotic-stresses-on-honeybee-physiology-and-colony-health#articles ). -- LICENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (http://creativecommons.org/licenses/by/4.0/ )
520 $aNutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the experimental variables on viral titers, including higher Deformed wing virus (DWV) titers associated with foraging and age-related decline. In addition, nutrition significantly affected DWV titers in young nurses, with higher titers induced by pollen ingestion. In contrast, higher levels of Black queen cell virus (BQCV) were associated with pollen restriction. Finally, correlation, PCA, and NMDS analyses proved that behavior had had the strongest effect on gene expression and viral titers, followed by age and nutrition. These analyses also support multiple interactions among genes and virus analyzed, including negative correlations between the expression of genes encoding storage proteins associated with pollen ingestion and nursing (vg and mrjp1) with the expression of immune genes and DWV titers. Our results provide new insights into the proximal mechanisms by which nutritional stress is associated with changes in honey bee physiology, immunity, and viral titers. Copyright © 2023 Corona, Branchiccela, Alburaki, Palmer-Young, Madella, Chen and Evans.
650 $aAPICULTURA
650 $aAPIS MELLIFERA
653 $aDorsal
653 $aDWV
653 $aImmune genes
653 $aInsulin
653 $aMFE
653 $aMRJP1
653 $aVitellogenin
700 1 $aBRANCHICCELA, B.
700 1 $aALBURAKI, M.
700 1 $aPALMER-YOUNG , E.C.
700 1 $aMADELLA , S.
700 1 $aCHEN, Y.
700 1 $aEVANS, J.D.
773 $tFrontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840
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| Registros recuperados : 25 | |
| 21. |  | BRANCHICCELA, B.; CASTELLI, L.; DIAZ, S.; INVERNIZZI, C; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTUNEZ, K, Can pollen supplementation mitigate the impact of nutritional stress on honey bee colonies? Journal of Apicultural Research, 2023, Volume 62, Issue 2, pp. 294-302. Doi: https://doi.org/10.1080/00218839.2021.1888537 Article history:Received 27 May 2020/ Accepted 08 Dec 2020/ Published online: 11 Mar 2021.Corresponding author. Email: bbranchiccela@inia.org.uy. This work was supported by the University of the Republic,Uruguay; under grant CSIC C624 and...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 22. | | CORONA, M.; BRANCHICCELA, B.; ALBURAKI, M.; PALMER-YOUNG , E.C.; MADELLA , S.; CHEN, Y.; EVANS, J.D. Decoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health. Original Research article. Frontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840 Article history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov -- Edited by: Zhiguo Li, Fujian Agriculture and Forestry University,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 23. | | INVERNIZZI, C.; ANTÚNEZ, K.; ARREDONDO, D.; BRANCHICCELA, B.; CASTELLI, L.; JURI, P.; MENDOZA, Y.; NOGUEIRA, E.; SALVARREY, S.; SANTOS, E. Situación sanitaria de las abejas melíferas en Uruguay: novedades de la última década. [Sanitary situation of honey bees in Uruguay: novelties of the last decade.]. Sección: Revisión. Veterinaria (Montev.), 2022, vol. 58, n. 217, e20225821704. https://doi.org/10.29155/VET.58.217.4 --- OPEN ACCESS. Article history: Recibido 05 Agosto 2021, Aceptado 23 Febrero 2022, Publicado 20 Mayo 2022. -- Autor para correspondencia: ciro@fcien.edu.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 24. | | BRANCHICCELA, B.; CASTELLI, L.; CORONA , M.; DIAZ-CETTI, S.; INVERNIZZI, C.; MARTÍNEZ DE LA ESCALERA, G; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTÚNEZ, K. Impact of nutritional stress on the honeybee colony health. Scientific Reports, November 2019, volume 9, issue 1. OPEN ACCESS Article history: Received 28 January 2019/Accepted 20 June 2019/Published 12 July 2019.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 25. | | REQUIER, F.; LEYTON, M.S.; MORALES, C.L.; GARIBALDI, L.A.; GIACOBINO, A.; PORRINI, M.P.; ROSSO-LONDOÑO, J.M.; VELARDE, R.A.; AIGNASSE, A.; ALDEA-SÁNCHEZ, P.; ALLASINO, M.L.; ARREDONDO, D.; AUDISIO, C.; CAGNOLO, N.B.; BASUALDO, M.; BRANCHICCELA, B.; CALDERÓN, R.A.; CASTELLI, L.; CASTILHOS, D.; ESCAREÑO, F.C.; CORREA-BENÍTEZ, A.; DA SILVA, F.O.; GARNICA, D.S.; DE GROOT, G.; DELGADO-CAÑEDO, A.; FERNÁNDEZ-MARÍN, H.; FREITAS, B.M.; GALINDO-CARDONA, A.; GARCIA, N.; GARRIDO, P.M.; GIRAY, T.; GONÇALVES, L.S.; LANDI, L.; MALUSÁ GONÇALVES, D.; MARTINEZ, S.I.; MOJA, P.J.; MOLINERI, A.; MÜLLER, P.F.; NOGUEIRA, E.; PACINI, A.; PALACIO, MARÍA ALEJANDRA; PARRA, G.N.; PARRA-H, A.; PERES GRAMACHO, K.; CASTRO, ELEAZAR PÉREZ; PIRES, C.S.S.; REYNALDI, F.J.; LUIS, A.R.; ROSSINI, C.; SÁNCHEZ ARMIJOS, M.; SANTOS, E.; SCANNAPIECO, A.; MENDOZA, Y.; TAPIA GONZÁLEZ, J.M.; VARGAS FERNÁNDEZ, ANDRÉS MARCELO; VIANA, B.F.; VIELI, L.; YADRÓ GARCÍA, C.A.; ANTÚNEZ, K. First large-scale study reveals important losses of managed honey bee and stingless bee colonies in Latin America. Scientific Reports, 2024, Volume 14, Issue 1, 10079. https://doi.org/10.1038/s41598-024-59513-6 -- OPEN ACCESS. Article history: Received 23 September 2023, Accepted 11 April 2024, Published 02 May 2024. -- Correspondence: Requier, F.; Université Paris-Saclay, CNRS, IRD, UMR Évolution, Génomes, Comportement et Écologie, Gif-sur-Yvette, France;...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 25 | |
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