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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
20/10/2017 |
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Actualizado : |
20/10/2017 |
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Tipo de producción científica : |
Poster |
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Autor : |
SIMETO, S.; CORALLO, B.; LUPO, S.; BETTUCCI, L.; GÓMEZ, D.; GONZALEZ, P.; MARTÍNEZ, G.; ABREO, E.; RIVAS, F.; ALTIER, N. |
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Afiliación : |
SOFIA SIMETO FERRARI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAULA KARINA GONZALEZ CASTELLI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GONZALO ANIBAL MARTINEZ CROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Biological control of Thaumastocoris peregrinus Carpintero and Dellappé (Heteroptera: Thaumastocoridae): towards the development of a mycoinsecticide. [Resumen]. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
In: Anniversary Congress, 125th, 19-22 September, Freiburg, Germany, 2017. |
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Páginas : |
p. 187 |
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Idioma : |
Inglés |
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Contenido : |
The bronze bug, Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae) is a small sap feeding hemipteran bug that has become
a serious pest of Eucalyptus plantations worldwide. Affected trees display foliage blight and defoliation, thus reducing photosynthesis and sometimes leading to the death of those highly infested mature trees. The use of entomopathogenic fungi as biological control agents is seen as an environmentally friendly alternative for pest control being chemical control seldom used in commercial forestry because its environmental and economical disadvantages. In this study, naturally occurring entomopathogenic fungi on T. peregrinus were isolated from dead and mycosed insects found in Eucalyptus plantations in Uruguay. Fungal species were identified based on spore and reproductive structures, morphology, culture characteristics, and molecular identification by amplification of the ribosomal DNA comprising ITS1, ITS2 and the 5.8S subunit. The identified entomopathogenic species belong to the genera Beauveria, Isaria, Lecanicillium, Purpureocillium and Pochonia. Bioassays using fiftyeight entomopathogenic fungal isolates from bronze bug and other forestry and agricultural pest insects were performed to determine their pathogenicity and virulence against T. peregrinus. A first in vitro screening was made by spraying 10 7 conidia/ml suspensions onto adults of T. peregrinus reared in mesh cages on detached leaf- bearing twigs of E. tereticornis. Isolates were classified into four categories based on the number of days to reach 90% of mortality. Most of the tested strains (80%) were pathogenic to bronze bug showing different degrees of virulence. For a subset of strains, values of LC 50 and LT50 were determined. In addition, the most promising strains are being subjected to mass production studies in order to identify cost-effective production methods and to select the most stable and virulent formulated strains. MenosThe bronze bug, Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae) is a small sap feeding hemipteran bug that has become
a serious pest of Eucalyptus plantations worldwide. Affected trees display foliage blight and defoliation, thus reducing photosynthesis and sometimes leading to the death of those highly infested mature trees. The use of entomopathogenic fungi as biological control agents is seen as an environmentally friendly alternative for pest control being chemical control seldom used in commercial forestry because its environmental and economical disadvantages. In this study, naturally occurring entomopathogenic fungi on T. peregrinus were isolated from dead and mycosed insects found in Eucalyptus plantations in Uruguay. Fungal species were identified based on spore and reproductive structures, morphology, culture characteristics, and molecular identification by amplification of the ribosomal DNA comprising ITS1, ITS2 and the 5.8S subunit. The identified entomopathogenic species belong to the genera Beauveria, Isaria, Lecanicillium, Purpureocillium and Pochonia. Bioassays using fiftyeight entomopathogenic fungal isolates from bronze bug and other forestry and agricultural pest insects were performed to determine their pathogenicity and virulence against T. peregrinus. A first in vitro screening was made by spraying 10 7 conidia/ml suspensions onto adults of T. peregrinus reared in mesh cages on detached leaf- bearing twigs of E. tereticornis. Isolates were cla... Presentar Todo |
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Thesagro : |
CONTROL BIOLÓGICO; FORESTACIÓN. |
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Asunto categoría : |
H01 Protección de plantas - Aspectos generales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7395/1/sIMETO-2017.pdf
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Marc : |
LEADER 02749nam a2200253 a 4500
001 1057688
005 2017-10-20
008 2017 bl uuuu u00u1 u #d
100 1 $aSIMETO, S.
245 $aBiological control of Thaumastocoris peregrinus Carpintero and Dellappé (Heteroptera$bThaumastocoridae): towards the development of a mycoinsecticide. [Resumen].$h[electronic resource]
260 $aIn: Anniversary Congress, 125th, 19-22 September, Freiburg, Germany$c2017
300 $ap. 187
520 $aThe bronze bug, Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae) is a small sap feeding hemipteran bug that has become a serious pest of Eucalyptus plantations worldwide. Affected trees display foliage blight and defoliation, thus reducing photosynthesis and sometimes leading to the death of those highly infested mature trees. The use of entomopathogenic fungi as biological control agents is seen as an environmentally friendly alternative for pest control being chemical control seldom used in commercial forestry because its environmental and economical disadvantages. In this study, naturally occurring entomopathogenic fungi on T. peregrinus were isolated from dead and mycosed insects found in Eucalyptus plantations in Uruguay. Fungal species were identified based on spore and reproductive structures, morphology, culture characteristics, and molecular identification by amplification of the ribosomal DNA comprising ITS1, ITS2 and the 5.8S subunit. The identified entomopathogenic species belong to the genera Beauveria, Isaria, Lecanicillium, Purpureocillium and Pochonia. Bioassays using fiftyeight entomopathogenic fungal isolates from bronze bug and other forestry and agricultural pest insects were performed to determine their pathogenicity and virulence against T. peregrinus. A first in vitro screening was made by spraying 10 7 conidia/ml suspensions onto adults of T. peregrinus reared in mesh cages on detached leaf- bearing twigs of E. tereticornis. Isolates were classified into four categories based on the number of days to reach 90% of mortality. Most of the tested strains (80%) were pathogenic to bronze bug showing different degrees of virulence. For a subset of strains, values of LC 50 and LT50 were determined. In addition, the most promising strains are being subjected to mass production studies in order to identify cost-effective production methods and to select the most stable and virulent formulated strains.
650 $aCONTROL BIOLÓGICO
650 $aFORESTACIÓN
700 1 $aCORALLO, B.
700 1 $aLUPO, S.
700 1 $aBETTUCCI, L.
700 1 $aGÓMEZ, D.
700 1 $aGONZALEZ, P.
700 1 $aMARTÍNEZ, G.
700 1 $aABREO, E.
700 1 $aRIVAS, F.
700 1 $aALTIER, N.
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Registro original : |
INIA Tacuarembó (TBO) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
13/11/2015 |
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Actualizado : |
25/09/2016 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
LADO, J.; RODRIGO, M.J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Transcriptional regulation of ethylene metabolism in 'Marsh' and 'Star Ruby' grapefruits under chilling conditions. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Acta Horticulturae, 2015, no.1079, p. 501-506. |
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ISBN : |
978-946261071-2 |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2015.1079.66 |
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Idioma : |
Inglés |
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Notas : |
Proc. Vth International Conference Postharvest Unlimited. Eds.: Toivonen P.M.,Kalaitzis P.,Manganaris G.A. |
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Contenido : |
ABSTRACT.
Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold
storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expression of the ETR genes. These results illustrate the complex regulation of the different members of ethylene biosynthetic, signaling and response pathway by cold stress in the peel of grapefruits. MenosABSTRACT.
Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold
storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expr... Presentar Todo |
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Thesagro : |
CITRUS; ESTRÉS DE FRÍO; ETILENO; TECNOLOGIA DE POSTCOSECHA; TRASTORNOS FUNCIONALES. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02533nga a2200253 a 4500
001 1053876
005 2016-09-25
008 2015 bl uuuu u00u1 u #d
020 $a978-946261071-2
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2015.1079.66$2DOI
100 1 $aLADO, J.
245 $aTranscriptional regulation of ethylene metabolism in 'Marsh' and 'Star Ruby' grapefruits under chilling conditions.
260 $c2015
500 $aProc. Vth International Conference Postharvest Unlimited. Eds.: Toivonen P.M.,Kalaitzis P.,Manganaris G.A.
520 $aABSTRACT. Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expression of the ETR genes. These results illustrate the complex regulation of the different members of ethylene biosynthetic, signaling and response pathway by cold stress in the peel of grapefruits.
650 $aCITRUS
650 $aESTRÉS DE FRÍO
650 $aETILENO
650 $aTECNOLOGIA DE POSTCOSECHA
650 $aTRASTORNOS FUNCIONALES
700 1 $aRODRIGO, M.J.
700 1 $aZACARÍAS, L.
773 $tActa Horticulturae, 2015, no.1079, p. 501-506.
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