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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
15/01/2020 |
Actualizado : |
05/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTELLS, M.; BERTONI, ESTEFANY; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; VICTORIA, M.; RIET-CORREA, F.; GIANNITTI, F.; PARREÑO, V.; COLINA, R. |
Afiliación : |
MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; ESTEFANY BERTONI, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATIAS VICTORIA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIVIANA PARREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICV y A, INTA Castelar, Nicolás Repetto S/N, Buenos Aires 1686, Argentina.; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay. |
Título : |
Bovine astrovirus surveillance in Uruguay reveals high detection rate of a novel mamastrovirus species. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Viruses, Dec 27, Vol. 12 , n.1, 2020. OPEN ACCESS. Doi: https://doi.org/10.3390/v12010032 |
DOI : |
10.3390/v12010032 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 26 October 2019 / Revised: 15 November 2019 / Accepted: 15 November 2019 / Published: 27 December 2019. Supplementary Materials: The following are available online at http://www.mdpi.com/1999-4915/12/1/32/s1.Figure S1: Maximum-likelihood tree constructed with complete capsid nucleotide sequences of MAstVs, Figure S2: Maximum-likelihood tree constructed with partial capsid amino acid sequences, Table S1: Partial polymerase sequences used for the phylogenetic analysis, and Table S2: Complete capsid sequences used for the MAstV
species classification.Author Contributions: Conceptualization, M.C. and R.C.; methodology, M.C., E.B., R.D.C., M.L.C. and C.S.;resources, M.C., F.R.-C., F.G. and R.C.; writing?original draft preparation, M.C.; writing?review and editing,M.C., E.B., R.D.C., M.L.C., C.S., M.V., F.R.-C., F.G., V.P. and R.C.; funding acquisition, M.C. and R.C. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by ?Comisión Sectorial de Investigación Científica? (CSIC), grant number ini2017_158 and ?Instituto Nacional de Investigación Agropecuaria? (INIA), grant number PL_015 N-15156. Acknowledgments: M.C. acknowledges support from the ?Agencia Nacional de Investigación e Innovación? (ANII) through a PhD scholarship. |
Contenido : |
Abstract:
Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the Mamastrovirus genus were identified, including Mamastrovirus 28, Mamastrovirus 33 (3 samples each) and an unclassified Mamastrovirus species (19 samples). The unclassified species was characterized as a novel Mamastrovirus species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation. |
Palabras claves : |
BOVINE ASTROVIRUS; DAIRY CATTLE; GENETIC DIVERSITY; MAMASTROVIRUS SPECIES; PLATAFORMA DE SALUD ANIMAL; PREVALENCE. |
Thesagro : |
URUGUAY. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16691/1/viruses-12-00032.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019600/pdf/viruses-12-00032.pdf
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Marc : |
LEADER 03407naa a2200337 a 4500 001 1060609 005 2022-09-05 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3390/v12010032$2DOI 100 1 $aCASTELLS, M. 245 $aBovine astrovirus surveillance in Uruguay reveals high detection rate of a novel mamastrovirus species.$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 26 October 2019 / Revised: 15 November 2019 / Accepted: 15 November 2019 / Published: 27 December 2019. Supplementary Materials: The following are available online at http://www.mdpi.com/1999-4915/12/1/32/s1.Figure S1: Maximum-likelihood tree constructed with complete capsid nucleotide sequences of MAstVs, Figure S2: Maximum-likelihood tree constructed with partial capsid amino acid sequences, Table S1: Partial polymerase sequences used for the phylogenetic analysis, and Table S2: Complete capsid sequences used for the MAstV species classification.Author Contributions: Conceptualization, M.C. and R.C.; methodology, M.C., E.B., R.D.C., M.L.C. and C.S.;resources, M.C., F.R.-C., F.G. and R.C.; writing?original draft preparation, M.C.; writing?review and editing,M.C., E.B., R.D.C., M.L.C., C.S., M.V., F.R.-C., F.G., V.P. and R.C.; funding acquisition, M.C. and R.C. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by ?Comisión Sectorial de Investigación Científica? (CSIC), grant number ini2017_158 and ?Instituto Nacional de Investigación Agropecuaria? (INIA), grant number PL_015 N-15156. Acknowledgments: M.C. acknowledges support from the ?Agencia Nacional de Investigación e Innovación? (ANII) through a PhD scholarship. 520 $aAbstract: Viral infections affecting cattle lead to economic losses to the livestock industry worldwide, but little is known about the circulation, pathogenicity and genetic diversity of enteric bovine astrovirus (BoAstV) in America. The aim of this work was to describe the prevalence and genetic diversity of enteric BoAstV in dairy cattle in Uruguay. A total of 457 fecal and 43 intestinal contents from dairy calves were collected between July 2015 and May 2017 and tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase and capsid regions. Twenty-six percent (128/500) of the samples were positive. Three different species within the Mamastrovirus genus were identified, including Mamastrovirus 28, Mamastrovirus 33 (3 samples each) and an unclassified Mamastrovirus species (19 samples). The unclassified species was characterized as a novel Mamastrovirus species. BoAstV circulates in Uruguayan dairy cattle with a high genetic diversity. The eventual clinicopathological significance of enteric BoAstV infection in cattle needs further investigation. 650 $aURUGUAY 653 $aBOVINE ASTROVIRUS 653 $aDAIRY CATTLE 653 $aGENETIC DIVERSITY 653 $aMAMASTROVIRUS SPECIES 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aPREVALENCE 700 1 $aBERTONI, ESTEFANY 700 1 $aCAFFARENA, D. 700 1 $aCASAUX, M.L. 700 1 $aSCHILD, C. 700 1 $aVICTORIA, M. 700 1 $aRIET-CORREA, F. 700 1 $aGIANNITTI, F. 700 1 $aPARREÑO, V. 700 1 $aCOLINA, R. 773 $tViruses, Dec 27, Vol. 12$gn.1, 2020. OPEN ACCESS. Doi: https://doi.org/10.3390/v12010032
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
21/02/2014 |
Actualizado : |
22/03/2021 |
Tipo de producción científica : |
Tesis |
Autor : |
CABRERA, D. |
Afiliación : |
CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Maturation and ripening of "Doyenne du comice" pears. [Thesis Master of Applied Science - M. Appl. Sc.] |
Fecha de publicación : |
1998 |
Fuente / Imprenta : |
Palmerston North (New Zealand): Massey University, 1998. |
Páginas : |
103 p. |
Idioma : |
Inglés |
Notas : |
A thesis presented in partial fulfilment of the requirements for the degree of Master of Applied Science in Horticultural Science at Massey University, Palmerston North, New Zealand. |
Contenido : |
ABSTRACT.
Characterisation of fruit quality attributes before and at harvest, during coolstorage and during ripening was made using standard and new, non-destructive devices during both the 1996 and 1997 seasons. Fruit firmness was linearly related to time when measured either by 'Kiwifirm' or penetrometer before harvest. Destructive techniques, the penetrometer and the texture analyser, were used to measure firmness and compared with non-destructive devices, the Kiwifirm and the softness meter. It is suggested that expressing rates of softening will be much more straightforward using a device such as the Kiwifirm. This device and the softness meter provided firmness data for pears that were too soft to measure by penetrometer. The effects of harvest date (1,11 and 21 March, 1996) and three crop loads on fruit maturity after a period of 6 weeks in coolstorage were investigated. Fruit size increased considerably during the 20 days before harvest, suggesting that periodical harvests need to be made in order to pick optimum size fruit each time. Maturity at harvest influenced the quality of 'Comice' stored at 0°C in air. Fruit from different harvests behaved differently in terms of softening behaviour and colour changes after 6 weeks in coolstorage. Crop load did not affect fruit quality attributes assessed after coolstorage. The characterisation of the nature and degree of within-tree and between tree fruit variability in harvest maturity and final ripening behaviour of 'Doyenne du Comice' pear was assessed by measuring firmness and colour. These attributes were measured non-destructively on fruit from different positions on the trees, and subsequently measured at harvest and during ripening at 20°C after 7 weeks in coolstorage at 0°C in air. Fruit behaved differently in terms of softening behaviour and colour changes depending on their position on the tree. Fruit maturity was delayed when fruit came from shaded areas, fruit from inner locations were greener than fruit from the outside and top positions. Selective picking and the association of harvest and ripening data may be important in making predictions that could reduce variability in fruit quality in the market place. MenosABSTRACT.
Characterisation of fruit quality attributes before and at harvest, during coolstorage and during ripening was made using standard and new, non-destructive devices during both the 1996 and 1997 seasons. Fruit firmness was linearly related to time when measured either by 'Kiwifirm' or penetrometer before harvest. Destructive techniques, the penetrometer and the texture analyser, were used to measure firmness and compared with non-destructive devices, the Kiwifirm and the softness meter. It is suggested that expressing rates of softening will be much more straightforward using a device such as the Kiwifirm. This device and the softness meter provided firmness data for pears that were too soft to measure by penetrometer. The effects of harvest date (1,11 and 21 March, 1996) and three crop loads on fruit maturity after a period of 6 weeks in coolstorage were investigated. Fruit size increased considerably during the 20 days before harvest, suggesting that periodical harvests need to be made in order to pick optimum size fruit each time. Maturity at harvest influenced the quality of 'Comice' stored at 0°C in air. Fruit from different harvests behaved differently in terms of softening behaviour and colour changes after 6 weeks in coolstorage. Crop load did not affect fruit quality attributes assessed after coolstorage. The characterisation of the nature and degree of within-tree and between tree fruit variability in harvest maturity and final ripening behaviour of 'Doyen... Presentar Todo |
Palabras claves : |
HARVESTING; PEARS; STORAGE; THESIS. |
Thesagro : |
ALMACENAMIENTO EN FRIO; FISIOLOGIA POSTCOSECHA; MADURAMIENTO; PERA; VARIEDADES. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03047nam a2200241 a 4500 001 1001478 005 2021-03-22 008 1998 bl uuuu m 00u1 u #d 100 1 $aCABRERA, D. 245 $aMaturation and ripening of "Doyenne du comice" pears. [Thesis Master of Applied Science - M. Appl. Sc.] 260 $aPalmerston North (New Zealand): Massey University$c1998 300 $a103 p. 500 $aA thesis presented in partial fulfilment of the requirements for the degree of Master of Applied Science in Horticultural Science at Massey University, Palmerston North, New Zealand. 520 $aABSTRACT. Characterisation of fruit quality attributes before and at harvest, during coolstorage and during ripening was made using standard and new, non-destructive devices during both the 1996 and 1997 seasons. Fruit firmness was linearly related to time when measured either by 'Kiwifirm' or penetrometer before harvest. Destructive techniques, the penetrometer and the texture analyser, were used to measure firmness and compared with non-destructive devices, the Kiwifirm and the softness meter. It is suggested that expressing rates of softening will be much more straightforward using a device such as the Kiwifirm. This device and the softness meter provided firmness data for pears that were too soft to measure by penetrometer. The effects of harvest date (1,11 and 21 March, 1996) and three crop loads on fruit maturity after a period of 6 weeks in coolstorage were investigated. Fruit size increased considerably during the 20 days before harvest, suggesting that periodical harvests need to be made in order to pick optimum size fruit each time. Maturity at harvest influenced the quality of 'Comice' stored at 0°C in air. Fruit from different harvests behaved differently in terms of softening behaviour and colour changes after 6 weeks in coolstorage. Crop load did not affect fruit quality attributes assessed after coolstorage. The characterisation of the nature and degree of within-tree and between tree fruit variability in harvest maturity and final ripening behaviour of 'Doyenne du Comice' pear was assessed by measuring firmness and colour. These attributes were measured non-destructively on fruit from different positions on the trees, and subsequently measured at harvest and during ripening at 20°C after 7 weeks in coolstorage at 0°C in air. Fruit behaved differently in terms of softening behaviour and colour changes depending on their position on the tree. Fruit maturity was delayed when fruit came from shaded areas, fruit from inner locations were greener than fruit from the outside and top positions. Selective picking and the association of harvest and ripening data may be important in making predictions that could reduce variability in fruit quality in the market place. 650 $aALMACENAMIENTO EN FRIO 650 $aFISIOLOGIA POSTCOSECHA 650 $aMADURAMIENTO 650 $aPERA 650 $aVARIEDADES 653 $aHARVESTING 653 $aPEARS 653 $aSTORAGE 653 $aTHESIS
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