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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
19/06/2019 |
Actualizado : |
05/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H. |
Afiliación : |
Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Ministério da Saúde, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil Silva, D.N. // Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Laboratory of Bacteriology and Health, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Saude Coletiva, Federal University of Bahia, Salvador, Brazil.; Instituto de Ciências da Saúde, Federal University of Bahia, Salvador, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil. |
Título : |
Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391 |
DOI : |
10.1371/journal.pone.0217391 |
Idioma : |
Inglés |
Notas : |
Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
Contenido : |
ABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. MenosABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected shee... Presentar Todo |
Palabras claves : |
DISEASES OF SHEEP; LEPTOSPIRES; PLATAFORMA EN SALUD ANIMAL. |
Thesagro : |
ENFERMEDADES DE LOS ANIMALES; LEPTOSPIROSIS. |
Asunto categoría : |
E16 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16705/1/pone.0217391.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/pdf/pone.0217391.pdf
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Marc : |
LEADER 03023naa a2200301 a 4500 001 1059867 005 2022-09-05 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0217391$2DOI 100 1 $aALMEIDA, D.S. 245 $aInvestigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.$h[electronic resource] 260 $c2019 500 $aArticle history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 520 $aABSTRACT. The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. 650 $aENFERMEDADES DE LOS ANIMALES 650 $aLEPTOSPIROSIS 653 $aDISEASES OF SHEEP 653 $aLEPTOSPIRES 653 $aPLATAFORMA EN SALUD ANIMAL 700 1 $aPAZ, L.N. 700 1 $aDE OLIVEIRA, D.S. 700 1 $aRISTOW, P. 700 1 $aHAMOND, C. 700 1 $aCOSTA, F. 700 1 $aPORTELA, R.W. 700 1 $aESTRELA-LIMA, A. 700 1 $aPINNA, M.H. 773 $tPloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391
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Registro original : |
INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Salto Grande. |
Fecha actual : |
28/06/2018 |
Actualizado : |
28/06/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
RUBIO, L.; AMARAL, J. |
Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN ANTONIO AMARAL SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Evaluación de productos de origen químico y biológico para el control de nematodos. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
In: INIA Salto Grande; Programa Nacional Producción Hortícola. Avances de investigación protección vegetal en tomate y morrón. Salto (UY): INIA, 2014. |
Páginas : |
p. 14-17 |
Serie : |
(Serie Actividades de Difusión; 740) |
ISSN : |
1688-9258 |
Idioma : |
Español |
Contenido : |
El uso intensivo de los invernaderos ha promovido un incremento en la incidencia y severidad de los nematodos (M. incognita), tornándose en una problemática de difícil control en algunas áreas de la zona hortícola de Salto. En el intento por obtener una mayor rentabilidad del sistema hortícola, es cada vez más complejo tener el suelo sin producir, por lo que se hace difícil cortar el ciclo de vida de esta plaga, ya que la mayoría de los cultivos utilizados son sus hospederos. |
Thesagro : |
CONTROL DE NEMATODOS; CULTIVO PROTEGIDO; TOMATE. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10552/1/sad-740-p.-14-17.pdf
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Marc : |
LEADER 01199naa a2200205 a 4500 001 1058754 005 2018-06-28 008 2014 bl uuuu u00u1 u #d 022 $a1688-9258 100 1 $aRUBIO, L. 245 $aEvaluación de productos de origen químico y biológico para el control de nematodos.$h[electronic resource] 260 $c2014 300 $ap. 14-17 490 $a(Serie Actividades de Difusión; 740) 520 $aEl uso intensivo de los invernaderos ha promovido un incremento en la incidencia y severidad de los nematodos (M. incognita), tornándose en una problemática de difícil control en algunas áreas de la zona hortícola de Salto. En el intento por obtener una mayor rentabilidad del sistema hortícola, es cada vez más complejo tener el suelo sin producir, por lo que se hace difícil cortar el ciclo de vida de esta plaga, ya que la mayoría de los cultivos utilizados son sus hospederos. 650 $aCONTROL DE NEMATODOS 650 $aCULTIVO PROTEGIDO 650 $aTOMATE 700 1 $aAMARAL, J. 773 $tIn: INIA Salto Grande; Programa Nacional Producción Hortícola. Avances de investigación protección vegetal en tomate y morrón. Salto (UY): INIA, 2014.
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