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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
21/10/2021 |
Actualizado : |
21/10/2021 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
MENA, E.; GARAYCOCHEA, S.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
Afiliación : |
EILYN MENA MÉNDEZ, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. ; Centro de Investigaciones Nucleares, Facultad de Ciencias, Montevideo, Uruguay.; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Secuenciación del genoma y perfil transcriptómico de Diaporthe caulovora, agente causal del cancro del tallo en soja. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
In: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2021. |
Páginas : |
p. 18. |
Idioma : |
Español |
Palabras claves : |
CANCRO DEL TALLO; ENFERMEDADES DE LAS PLANTAS; SOJA. |
Asunto categoría : |
H20 Enfermedades de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16047/1/Garaycochea-1.pdf
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Marc : |
LEADER 00822nam a2200193 a 4500 001 1062459 005 2021-10-21 008 2021 bl uuuu u00u1 u #d 100 1 $aMENA, E. 245 $aSecuenciación del genoma y perfil transcriptómico de Diaporthe caulovora, agente causal del cancro del tallo en soja.$h[electronic resource] 260 $aIn: Sociedad Uruguaya de Fitopatología Jornada Uruguaya de Fitopatología, 6., Jornada Uruguaya de Protección Vegetal, 4., 21-22 octubre, 2021, Montevideo, Uruguay. Libro de resúmenes. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT)$c2021 300 $ap. 18. 653 $aCANCRO DEL TALLO 653 $aENFERMEDADES DE LAS PLANTAS 653 $aSOJA 700 1 $aGARAYCOCHEA, S. 700 1 $aSTEWART, S. 700 1 $aMONTESANO, M. 700 1 $aPONCE DE LEÓN, I.
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INIA Treinta y Tres (TT) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
22/01/2020 |
Actualizado : |
24/02/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
RODRIGO, M.J.; LADO, J.; ALÓS, E.; ALQUÉZAR, B.; DERY, O.; HIRSCHBERG, J.; ZACARÍAS, L. |
Afiliación : |
MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; ENRIQUETA ALÓS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; BERTA ALQUÉZAR, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Spain; ORLY DERY, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; JOSEPH HIRSCHBERG, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain. |
Título : |
A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
BMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2 |
ISSN : |
1471-2229 |
DOI : |
10.1186/s12870-019-2078-2 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019.
Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
Supplementary information accompanies this paper. |
Contenido : |
Abstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits.
© 2019 The Author(s). MenosAbstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit an... Presentar Todo |
Palabras claves : |
Carotenoid; GENE EXPRESSION; MUTANT; Pigmentation; PLATAFORMA AGROALIMENTOS; RIPENING; Zeta-carotene isomerase. |
Thesagro : |
CAROTENOIDES; CITRUS. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14051/1/BMC-Plant-Pathology-2019-s12870-019-2078-2.pdf
https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-019-2078-2
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Marc : |
LEADER 04646naa a2200337 a 4500 001 1060661 005 2022-02-24 008 2019 bl uuuu u00u1 u #d 022 $a1471-2229 024 7 $a10.1186/s12870-019-2078-2$2DOI 100 1 $aRODRIGO, M.J. 245 $aA mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis.$h[electronic resource] 260 $c2019 500 $aArticle history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019. Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Supplementary information accompanies this paper. 520 $aAbstract. Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits. © 2019 The Author(s). 650 $aCAROTENOIDES 650 $aCITRUS 653 $aCarotenoid 653 $aGENE EXPRESSION 653 $aMUTANT 653 $aPigmentation 653 $aPLATAFORMA AGROALIMENTOS 653 $aRIPENING 653 $aZeta-carotene isomerase 700 1 $aLADO, J. 700 1 $aALÓS, E. 700 1 $aALQUÉZAR, B. 700 1 $aDERY, O. 700 1 $aHIRSCHBERG, J. 700 1 $aZACARÍAS, L. 773 $tBMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2
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