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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
08/06/2021 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GASTAL, G.D.A.; SCARLET D.; MELCHERT M.; ERTL R.; AURICH, C. |
Afiliación : |
GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./ Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; DRAGOS SCARLET, Division of Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria;; MARIA MELCHERT, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; REINHARD ERT, Vetcore Facility, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.; CHRISTINE AURICH, Center for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
Título : |
Epigenetic changes in equine embryos after short-term storage at different temperaturas. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Animals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325 |
DOI : |
10.3390/ani11051325 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. |
Contenido : |
Simple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim
of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development,
maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C);
day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation
(malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There
were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis
(BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and
DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest
some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. MenosSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern.
The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and
development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer.
Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and... Presentar Todo |
Palabras claves : |
Development; Embryo; Embryo-maternal recognition; Equine; Methylation; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Transport. |
Thesagro : |
EQUINOS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16671/1/animals-11-01325.pdf
https://www.mdpi.com/2076-2615/11/5/1325/pdf
|
Marc : |
LEADER 04973naa a2200301 a 4500 001 1062116 005 2022-09-02 008 2021 bl uuuu u00u1 u #d 024 7 $a10.3390/ani11051325$2DOI 100 1 $aGASTAL, G.D.A. 245 $aEpigenetic changes in equine embryos after short-term storage at different temperaturas.$h[electronic resource] 260 $c2021 500 $aArticle history: Received: 16 March 2021/Accepted: 3 May 2021/Published: 6 May 2021. This work was supported by University of Veterinary Medicine Vienna, Austria. 520 $aSimple Summary: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, concerning morphological development, expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation, apoptosis and gene-specific and global DNA methylation. Temperature during storage did not affect embryo size. There were no changes in pH and lipid peroxidation of the medium irrespective of group. mRNA expression and gene-specific DNA methylation of genes related to growth and development, maternal recognition of pregnancy, DNA methylation and apoptosis in stored embryos (5 ?C and 20 ?C) were altered when compared to fresh embryos. Therefore, our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Short-term storage, regardless of temperature, may compromise embryo development after transfer. Abstract: In embryos subjected to assisted reproductive techniques, epigenetic modifications may occur that can influence embryonic development and the establishment of pregnancy. In horses, the storage temperature during transport of fresh embryos before transfer is a major concern. The aim of this study was, therefore, to determine the effects of two storage temperatures (5 ?C and 20 ?C) on equine embryos, collected at day seven after ovulation and stored for 24 h, on: (i) morphological development; (ii) expression of candidate genes associated with embryo growth and development, maternal recognition of pregnancy, methylation and apoptosis, and (iii) gene-specific and global DNA methylation. Embryos (n = 80) were collected on day seven or day eight after ovulation and assigned to four groups: day seven control (E7F, fresh); day seven, stored for 24 h at 5 ?C (E5C); day seven, stored for 24 h at 20 ?C (E20C) and day eight control (E8F, fresh 24h time control). The embryos and the storage medium (EquiHold, holding medium, Minitube, Tiefenbach, Germany) from all treatment groups were analyzed for (i) medium temperature, pH, and lipid peroxidation (malondialdehyde; MDA) and (ii) embryo morphology, mRNA expression and DNA methylation (immunohistochemistry and gene-specific DNA methylation). The size of embryos stored at 5 ?C was larger (p < 0.01), whereas embryos stored at 20 ?C were smaller (p < 0.05) after 24 h. There were no changes in pH and MDA accumulation irrespective of the group. The mRNA expression of specific genes related to growth and development (POU5F1, SOX2, NANOG), maternal recognition of pregnancy (CYP19A1, PTGES2), DNA methylation (DNMT1, DNMT3A, DNMT3B) and apoptosis (BAX) in the E5C and E20C were either up or downregulated (p < 0.05) when compared to controls (E7F and E8F). The immune expression of 5mC and 5hmC was similar among treatment groups. Percentage of methylation in the CpG islands was lower in the specific genes ESR1, NANOG and DNMT1 (p < 0.001) in E20C embryos when compared to E8F (advanced embryo stage). Therefore,our study demonstrates for the first time the gene-specific and global DNA methylation status of fresh equine embryos collected on days seven and eight after ovulation. Although our results suggest some beneficial effects of storage at 20 ?C in comparison to 5 ?C, the short-term storage, regardless of temperature, modified gene expression and methylation of genes involved in embryo development and may compromise embryo viability and development after transfer. 650 $aEQUINOS 653 $aDevelopment 653 $aEmbryo 653 $aEmbryo-maternal recognition 653 $aEquine 653 $aMethylation 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aTransport 700 1 $aSCARLET D. 700 1 $aMELCHERT M. 700 1 $aERTL R. 700 1 $aAURICH, C. 773 $tAnimals, 5 May 2021, Volume 11, Issue 5, Article number 1325. Open Access. Doi: https://doi.org/10.3390/ani11051325
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INIA La Estanzuela (LE) |
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41. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | SARAVIA, A.; MIRABALLES, C.; RIET-CORREA, F.; CASTRO-JANER, E. Eimeria spp. in dairy calves in Uruguay. Identification, dynamics of oocyst excretion and association with the age of calves. Veterinary Parasitology: Regional Studies and Reports, 25 July 2021, Volume 25, Article number 100588. Doi: https://doi.org/10.1016/j.vprsr.2021.100588. Article history: Received 12 February 2020, Revised 19 February 2021, Accepted 12 May 2021, Available online 14 May 2021.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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44. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | VIEIRA, V.D.; RIET-CORREA, W.; VILELA, V.L.R.; MEDEIROS, M.A.; BATISTA, J.A.; MELO, L.R.B.; SANTOS, A.; RIET-CORREA, F. Controle de parasitas gastrintestinais em ovinos e análise financeira de uma fazenda com sistema de pastejo rotacionado irrigado no semiárido nordestino.(Control of gastrointestinal nematodes in sheep and financial analysis on a farm with irrigated rotational grazing system in the Brazilian semi-arid region), Pesquisa Veterinária Brasileira v.38 ,n.5. p. 913-919, 2018. OPEN ACCESS. Article History: Recebido em 22 de abril de 2017.//Aceito para publicação em 29 de abril de 2017Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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46. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | DONCEL, B.; CASTELLS, M.; MAYA, L.; FRAGA, M.; UZAL, F.A; COLINA, R.; GIANNITTI, F. Encefalitis asociada a astrovirus bovino neurotrópico, ¿una enfermedad subdiagnosticada en Sudamérica?.[Neurotropic bovine astrovirus-associated encephalitis: An underdiagnosed disease in South America?]. Revista Argentina de Microbiologia, Volume 54, Pages 100-105, 2022. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2021.01.00 Article history: Recibido el 15 de julio de 2020/ Aaceptado el 24 de enero de 2021./Disponible en Internet el 18 junio 2021. Autor para correspondencia: Correo electrónico: fgiannitti@inia.org.uy (F. Giannitti). Este trabajo fue...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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47. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | MENDONÇA, F.S.; NASCIMENTO , N.C. F.; ALMEIDA, V.M.; BRAGA, T.C.; RIBEIRO, D.P.; CHAVES, H.A.S.; SILVA FILHO , G.B.; RIET-CORREA, F. An outbreak of poisoning by Kalanchoe blossfeldiana in cattle in northeastern Brazil. Tropical Animal Health and Production, 2018, v. 50, n. 3, p. 693-696. Article History: Received: 17 February 2017 /Accepted: 30 October 2017 / Published online: 16 November 2017.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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48. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | COSTA, R.A. DA; CAFFARENA, D.; MIRAZO, S.; DIAB, S.; CASAUX, L.; MAYA, L.; ARBIZA, J.; RIET-CORREA, F.; GIANNITTI, F. Neumonía por el virus respiratorio sincitial bovino (BRSV) en bovinos lecheros de Colonia, Uruguay. In: Jornadas Uruguayas de Buiatría, 45., 2017, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2017.Tipo: Trabajos en Congresos/Conferencias |
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49. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | MACÍAS-RIOSECO, M.; MIRAZO ,S.; UZAL, F.A.; FRAGA, M.; SILVEIRA, C.S.; MAYA, L.; RIET-CORREA, F.; ARBIZA, J.; COLINA, R.; ANDERSON ,M.L.; GIANNITTI, F. Fetal Pathology in an Aborted Holstein Fetus Infected With Bovine Parainfluenza Virus-3 Genotype A. Veterinary Pathology [Vet Pathol], 2018 Sep 24, p. 300985818798117. Article History:Date Created: 20180925 //Latest Revision: 20180924.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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50. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | SCHILD, C.; COSTA, R.A. DA; ARÁOZ, V.; INGOLD, A.; GARCIA Y SANTOS, C.; GIANNITTI, F.; RIET-CORREA, F. Aspergilosis pulmonar crónica cavitaria en una vaca Holstein en Uruguay. In: Jornadas Técnicas Veterinarias, 10º.,Facultad de Veterinaria, Universidad de la República: Montevideo, Uruguay,2017.Tipo: Trabajos en Congresos/Conferencias |
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52. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | RIVAS MATONTE, E.; PARODI, P.; MATTO, C.; GIANNECCHINI, E.; SCHANZEMBACH, M.; RODRÍGUEZ, V.; RIVERO, R. Dispersión de celos naturales posterior a sincronización con prostaglandinas en cuatro razas ovinas en condiciones de pastoreo. In: Jornadas Uruguayas de Buiatría, 48., 2021. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 31 de Mayo al 4 de Junio 2021. p. 264-266.Biblioteca(s): INIA La Estanzuela. |
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53. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | SILVEIRA, C.S.; ARMENDANO, J.I.; MOORE, D.P.; CANTÓN, G.J.; MACÍAS-RIOSECO, M.; RIET-CORREA, F.; GIANNITTI, F. A comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay]. Revista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004 Article history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019.
Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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55. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | MENCHACA, A. Assisted Reproductive Technologies (ART) and genome editing to support a sustainable livestock. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230074. http://dx.doi.org/10.1590/1984-3143-AR2023-0074 -- OPEN ACCESS. Article history: Received May 26, 2023; Accepted July 21, 2023. -- Document type: Article Gold Open Access, Green Open Access. -- Correspondence: Menchaca, A.; Plataforma de Salud Animal, Instituto Nacional de...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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58. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | NIEVES. C; HAMOND, C.; BURONI, F.; RIVERO, R.; SUANES, A.; SALABERRY, X.; MACÍAS-RIOSECO, M.; SILVEIRA, C.S.; GIANNITTI, F.; RIET-CORREA, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Aplicación de métodos moleculares para la identificación y genotipicación de especies patógenas de Leptospira en muestras clínicas de bovinos. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 207-210.Tipo: Trabajos en Congresos/Conferencias |
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59. | ![Imagen marcada / sin marcar](/consulta/web/img/desmarcado.png) | SCHILD, C.; CAFFARENA, D.; RIET-CORREA, F.; GIANNITTI, F. Enfermedad articular degenerativa de las rodillas en una vaca. In: JORNADAS URUGUAYAS DE BUIATRÍA, 43., 2015, PAYSANDÚ, UY.; GIANNEECHINI, E.; MATTO, C. (Ed.). [Trabajos presentados]. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría. p.221-223.Tipo: Trabajos en Congresos/Conferencias |
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