| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha : |
04/09/2019 |
|
Actualizado : |
16/03/2021 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
ROVIRA, P.J.; MCALLISTER, T.; LAKIN, S.M.; COOK, S.R.; DOSTER, E.; NOYES, N. R.; WEINROTH, M.D.; YANG, X.; PARKER, J. K.; BOUCHER, C.; BOOKER, C. W.; WOENER, D. R.; BELK, K. E.; MORLEY, P. S. |
|
Afiliación : |
PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; TIM MCALLISTER, Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada.; STEVEN M. LAKIN, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; SHAUN R. COOK, Alberta Agricultural and forestry, Lethbridge, Canada.; ENRIQUE DOSTER, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; NOELLE R. NOYES, Veterinary Population Medicine Department, University of Minnesota, USA.; MAGGIE D. WEINROTH, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; XIANG YANG, Department of Animal Science, University of California, Davis, USA.; JENNIFER K. PARKER, Deparment of Molecular Biosciences, University of Florida, Gainesville, FL, USA.; CHRISTINA BOUCHER, Deparment of Computer and Information Science and Engineering, University of Florida, Gainessville, FL, USA.; CALVIN W. BOOKER, Feedlot Health Management Services, Okotoks, AB, Canada.; DALE R. WOEMER, Deparment of Animal and Food Sciences, College of Agricultural Sciences & Natural Resources, Texas University, TX, USA.; KEITH E. BELK, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; PAUL S. MORLEY, VERO, Veterinary Education, Research , and Outreach Program, Texas A&M University and West Texas A&M University, Canyon, TX, USA. |
|
Título : |
Characterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems. |
|
Fecha de publicación : |
2019 |
|
Fuente / Imprenta : |
Frontier in Microbiology, September 2019. v. 10, article 1980, 11 p. OPEN ACCESS. |
|
DOI : |
10.3389/fmicb.2019.01980 |
|
Idioma : |
Inglés |
|
Notas : |
Received 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019. |
|
Contenido : |
Metagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance.
Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes. MenosMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle sour... Presentar Todo |
|
Palabras claves : |
ANTIBIOTIC RESISTANCE; CATTLE; CATTLE BEEF; DAIRY CATTLE; METAGENOMICA; METAGENOMICS; MICROBIOMA; MICROBIOME; RESISTENCIA A ANTIBIÓTICOS; RESISTOME. |
|
Thesagro : |
BOVINOS; BOVINOS DE CARNE; GANADO LECHERO. |
|
Asunto categoría : |
-- |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13237/1/Rovira-Front-microb-2019.pdf
|
|
Marc : |
LEADER 03681naa a2200457 a 4500
001 1060137
005 2021-03-16
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.01980$2DOI
100 1 $aROVIRA, P.J.
245 $aCharacterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems.$h[electronic resource]
260 $c2019
500 $aReceived 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019.
520 $aMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance. Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes.
650 $aBOVINOS
650 $aBOVINOS DE CARNE
650 $aGANADO LECHERO
653 $aANTIBIOTIC RESISTANCE
653 $aCATTLE
653 $aCATTLE BEEF
653 $aDAIRY CATTLE
653 $aMETAGENOMICA
653 $aMETAGENOMICS
653 $aMICROBIOMA
653 $aMICROBIOME
653 $aRESISTENCIA A ANTIBIÓTICOS
653 $aRESISTOME
700 1 $aMCALLISTER, T.
700 1 $aLAKIN, S.M.
700 1 $aCOOK, S.R.
700 1 $aDOSTER, E.
700 1 $aNOYES, N. R.
700 1 $aWEINROTH, M.D.
700 1 $aYANG, X.
700 1 $aPARKER, J. K.
700 1 $aBOUCHER, C.
700 1 $aBOOKER, C. W.
700 1 $aWOENER, D. R.
700 1 $aBELK, K. E.
700 1 $aMORLEY, P. S.
773 $tFrontier in Microbiology, September 2019.$gv. 10, article 1980, 11 p. OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
20/01/2022 |
|
Actualizado : |
18/03/2022 |
|
Autor : |
CLOTHIER, K.A.; WATSON, K.D.; METE, A.; GIANNITTI, F.; ANDERSON, M.; MUNK, B.; MCMILLIN, S.; CLIFFORD, D.L.; RUDD, J.; SHIRKEY, N.; FAMINI, D.; WOODS, L. |
|
Afiliación : |
KRISTIN A. CLOTHIER, California Animal Health and Food Safety Laboratory System, University of California-Davis, CA, USA.; KATHERINE D. WATSON, California Animal Health and Food Safety Laboratory System, University of California-Davis, CA, USA.; ASLI METE, California Animal Health and Food Safety Laboratory System, University of California-Davis, CA, USA.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; California Department of Fish and Wildlife, Rancho Cordova, CA, USA.; DAN FAMINI, Sonoma County Wildlife Rescue, Petaluma, CA, USA.; LESLIE WOODS, California Animal Health and Food Safety Laboratory System, University of California-Davis, CA, USA. |
|
Título : |
Generalized dermatophytosis caused by Trichophyton equinum in 8 juvenile black bears in California. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, 2022, Volume 34, Issue 2, pp.279-283. doi: https://doi.org/10.1177/10406387211061143 |
|
DOI : |
10.1177/10406387211061143 |
|
Idioma : |
Inglés |
|
Contenido : |
Abstract. From 2014?2019, 8 juvenile black bears (Ursus americanus) from different geographic regions were presented to the California Department of Fish and Wildlife because of emaciation, alopecia, and exfoliative dermatitis that resulted in death or euthanasia. Autopsy and histopathology revealed that all 8 bears had generalized hyperkeratotic dermatitis, folliculitis, and furunculosis. Skin structures were heavily colonized by fungal hyphae and arthrospores; fungal cultures of skin from 7 bears yielded Trichophyton equinum, a zoophilic dermatophyte reported only rarely in non-equid species. Additional skin conditions included mites (5), ticks (2), and coagulase-negative Staphylococcus sp. infections (2). No other causes of morbidity or mortality were identified. Molecular comparisons performed at the University of Texas Fungal Reference Laboratory determined that all isolates produced identical banding patterns, potentially representing a clonal population.
Dermatophytosis is commonly localized and limited to the stratum corneum of the epidermis and hair follicles. Generalized disease with dermal involvement is rare in immunocompetent individuals; illness, malnutrition, age, or immunosuppression may increase susceptibility. Underlying causes for the severe disease impact in these bears were not evident after physical or postmortem examination. The mechanism by which bears from different geographic locations had severe, T. equinum?associated dermatophytosis from a potentially clonal dermatophyte could not be explained and warrants further investigation. MenosAbstract. From 2014?2019, 8 juvenile black bears (Ursus americanus) from different geographic regions were presented to the California Department of Fish and Wildlife because of emaciation, alopecia, and exfoliative dermatitis that resulted in death or euthanasia. Autopsy and histopathology revealed that all 8 bears had generalized hyperkeratotic dermatitis, folliculitis, and furunculosis. Skin structures were heavily colonized by fungal hyphae and arthrospores; fungal cultures of skin from 7 bears yielded Trichophyton equinum, a zoophilic dermatophyte reported only rarely in non-equid species. Additional skin conditions included mites (5), ticks (2), and coagulase-negative Staphylococcus sp. infections (2). No other causes of morbidity or mortality were identified. Molecular comparisons performed at the University of Texas Fungal Reference Laboratory determined that all isolates produced identical banding patterns, potentially representing a clonal population.
Dermatophytosis is commonly localized and limited to the stratum corneum of the epidermis and hair follicles. Generalized disease with dermal involvement is rare in immunocompetent individuals; illness, malnutrition, age, or immunosuppression may increase susceptibility. Underlying causes for the severe disease impact in these bears were not evident after physical or postmortem examination. The mechanism by which bears from different geographic locations had severe, T. equinum?associated dermatophytosis from a potent... Presentar Todo |
|
Palabras claves : |
Bears; Dermatophytosis; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Trichophyton equinum; Ursus americanus. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02641naa a2200337 a 4500
001 1062648
005 2022-03-18
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1177/10406387211061143$2DOI
100 1 $aCLOTHIER, K.A.
245 $aGeneralized dermatophytosis caused by Trichophyton equinum in 8 juvenile black bears in California.$h[electronic resource]
260 $c2022
520 $aAbstract. From 2014?2019, 8 juvenile black bears (Ursus americanus) from different geographic regions were presented to the California Department of Fish and Wildlife because of emaciation, alopecia, and exfoliative dermatitis that resulted in death or euthanasia. Autopsy and histopathology revealed that all 8 bears had generalized hyperkeratotic dermatitis, folliculitis, and furunculosis. Skin structures were heavily colonized by fungal hyphae and arthrospores; fungal cultures of skin from 7 bears yielded Trichophyton equinum, a zoophilic dermatophyte reported only rarely in non-equid species. Additional skin conditions included mites (5), ticks (2), and coagulase-negative Staphylococcus sp. infections (2). No other causes of morbidity or mortality were identified. Molecular comparisons performed at the University of Texas Fungal Reference Laboratory determined that all isolates produced identical banding patterns, potentially representing a clonal population. Dermatophytosis is commonly localized and limited to the stratum corneum of the epidermis and hair follicles. Generalized disease with dermal involvement is rare in immunocompetent individuals; illness, malnutrition, age, or immunosuppression may increase susceptibility. Underlying causes for the severe disease impact in these bears were not evident after physical or postmortem examination. The mechanism by which bears from different geographic locations had severe, T. equinum?associated dermatophytosis from a potentially clonal dermatophyte could not be explained and warrants further investigation.
653 $aBears
653 $aDermatophytosis
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTrichophyton equinum
653 $aUrsus americanus
700 1 $aWATSON, K.D.
700 1 $aMETE, A.
700 1 $aGIANNITTI, F.
700 1 $aANDERSON, M.
700 1 $aMUNK, B.
700 1 $aMCMILLIN, S.
700 1 $aCLIFFORD, D.L.
700 1 $aRUDD, J.
700 1 $aSHIRKEY, N.
700 1 $aFAMINI, D.
700 1 $aWOODS, L.
773 $tJournal of Veterinary Diagnostic Investigation, 2022, Volume 34, Issue 2, pp.279-283. doi: https://doi.org/10.1177/10406387211061143
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
