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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
26/07/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
HIRIGOYEN, D.; BRUZZONE, H.; AZAMBUJA, C.; STOLL, M. |
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Afiliación : |
D. HIRIGOYEN, División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo.; H. BRUZZONE GIOVANELLI, División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo; CARLOS AZAMBUJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIO STOLL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; División Citogenética. Instituto de Investigaciones Biológicas "Clemente Estable" (IIBCE). Montevideo. |
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Título : |
Generación de marcadores macho específicos para la determinación de sexo bovino por PCR. |
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Complemento del título : |
Trabajo original. |
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Fecha de publicación : |
1994 |
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Fuente / Imprenta : |
Veterinaria, 1994, Vol. 29, no. 122, p. 12-18. --- OPEN ACCESS. |
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Idioma : |
Español |
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Notas : |
Article history: Aprobado 3 Mayo 1994. -- |
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Contenido : |
RESUMEN.- La determinación prenatal del sexo en las especies de interés pecuario, es una metodología de gran interés desde el punto de vista productivo. En este trabajo se utilizó el ADN de seis bovinos, raza Holando, para detectar marcadores macho específicos (sexado), con la técnica de amplificación de ADN in-vitro (PCR). De esta forma fue posible detectar secuencias específicas de sexo masculino, utilizando cantidades de muestras tan pequeñas como 5 pg de ADN. El método diagnóstico aplicado resultó ser específico, sensible, rapido y económico. -------------- SUMMARY.- The prenatal sex determination in farm animals species of interest, is an estrategic goal from a productive point of view. In this work the DNA amplification in vitro technique (PCR) was used to detect male specific markers (sex marker), using the DNA of six Holstein breed bovines. It was posible the detections of male markers DNA amplification using as little as 5 pg of DNA samples. The diagnostic method applied to bovine resulted specific, sensitive, rapid and economic. |
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Palabras claves : |
Amplificación de ADN; Cattle; Determinación del sexo; DNA amplification; Polymerase chain reaction; Sex determination. |
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Thesagro : |
BOVINOS; PCR. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17312/1/VM-1994-122-12-18.pdf
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Marc : |
LEADER 01868naa a2200265 a 4500
001 1003704
005 2023-07-26
008 1994 bl uuuu u00u1 u #d
100 1 $aHIRIGOYEN, D.
245 $aGeneración de marcadores macho específicos para la determinación de sexo bovino por PCR.$h[electronic resource]
260 $c1994
500 $aArticle history: Aprobado 3 Mayo 1994. --
520 $aRESUMEN.- La determinación prenatal del sexo en las especies de interés pecuario, es una metodología de gran interés desde el punto de vista productivo. En este trabajo se utilizó el ADN de seis bovinos, raza Holando, para detectar marcadores macho específicos (sexado), con la técnica de amplificación de ADN in-vitro (PCR). De esta forma fue posible detectar secuencias específicas de sexo masculino, utilizando cantidades de muestras tan pequeñas como 5 pg de ADN. El método diagnóstico aplicado resultó ser específico, sensible, rapido y económico. -------------- SUMMARY.- The prenatal sex determination in farm animals species of interest, is an estrategic goal from a productive point of view. In this work the DNA amplification in vitro technique (PCR) was used to detect male specific markers (sex marker), using the DNA of six Holstein breed bovines. It was posible the detections of male markers DNA amplification using as little as 5 pg of DNA samples. The diagnostic method applied to bovine resulted specific, sensitive, rapid and economic.
650 $aBOVINOS
650 $aPCR
653 $aAmplificación de ADN
653 $aCattle
653 $aDeterminación del sexo
653 $aDNA amplification
653 $aPolymerase chain reaction
653 $aSex determination
700 1 $aBRUZZONE, H.
700 1 $aAZAMBUJA, C.
700 1 $aSTOLL, M.
773 $tVeterinaria, 1994, Vol. 29, no. 122, p. 12-18. --- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/07/2021 |
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Actualizado : |
22/07/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CROPANO, C.; PLACE, I.; MANZANARES, C.; DO CANTO, J.; LÜBBERSTEDT, T.; STUDER, B.; THOROGOOD, D. |
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Afiliación : |
CLAUDIO CROPANO, Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland; Deutsche Saatveredelung AG, Lippstadt, Germany.; IAIN PLACE, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.; CHLOÉ MANZANARES, Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland; JAVIER DO CANTO FAGUNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; THOMAS LÜBBERSTEDT, Department of Agronomy, Iowa State University, Ames, IA, USA; BRUNO STUDER, Molecular Plant Breeding, Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland; DANIEL THOROGOOD, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK. |
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Título : |
Characterization and practical use of self-compatibility in outcrossing grass species. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Annals of Botany, June 2021, volume 127, Issue 7, Pages 841 - 8524. OPEN ACCESS. Doi: https://doi.org/10.1093/aob/mcab043 |
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ISSN : |
0305-7364 |
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DOI : |
10.1093/aob/mcab043 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 November 2020, Revision received 12 March 2021, Editorial decision 17 March 2021, Accepted 18 March 2021, Published 23 March 2021,
Corrected and typeset 24 June 2021.
Corresponding author: Thorogood, D.; Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United Kingdom; email:dnt@aber.ac.uk |
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Contenido : |
ABSTRACT - Background: Self-incompatibility (SI) systems prevent self-fertilization in several species of Poaceae, many of which are economically important forage, bioenergy and turf grasses. Self-incompatibility ensures cross-pollination and genetic diversity but restricts the ability to fix useful genetic variation. In most inbred crops it is possible to develop high-performing homozygous parental lines by self-pollination, which then enables the creation of F1 hybrid varieties with higher performance, a phenomenon known as heterosis. The inability to fully exploit heterosis in outcrossing grasses is partially responsible for lower levels of improvement in breeding programmes compared with inbred crops. However, SI can be overcome in forage grasses to create self-compatible populations. This is generating interest in understanding the genetical basis of self-compatibility (SC), its significance for reproductive strategies and its exploitation for crop improvement, especially in the context of F1 hybrid breeding. Scope: We review the literature on SI and SC in outcrossing grass species. We review the currently available genomic tools and approaches used to discover and characterize novel SC sources. We discuss opportunities barely explored for outcrossing grasses that SC facilitates. Specifically, we discuss strategies for wide SC introgression in the context of the Lolium-Festuca complex and the use of SC to develop immortalized mapping populations for the dissection of a wide range of agronomically important traits. The germplasm available is a valuable practical resource and will aid understanding the basis of inbreeding depression and hybrid vigour in key temperate forage grass species. Conclusions: A better understanding of the genetic control of additional SC loci offers new insight into SI systems, their evolutionary origins and their reproductive significance. Heterozygous outcrossing grass species that can be readily selfed facilitate studies of heterosis. Moreover, SC introduction into a range of grass species will enable heterosis to be exploited in innovative ways in genetic improvement programmes.
© 2021 The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company. MenosABSTRACT - Background: Self-incompatibility (SI) systems prevent self-fertilization in several species of Poaceae, many of which are economically important forage, bioenergy and turf grasses. Self-incompatibility ensures cross-pollination and genetic diversity but restricts the ability to fix useful genetic variation. In most inbred crops it is possible to develop high-performing homozygous parental lines by self-pollination, which then enables the creation of F1 hybrid varieties with higher performance, a phenomenon known as heterosis. The inability to fully exploit heterosis in outcrossing grasses is partially responsible for lower levels of improvement in breeding programmes compared with inbred crops. However, SI can be overcome in forage grasses to create self-compatible populations. This is generating interest in understanding the genetical basis of self-compatibility (SC), its significance for reproductive strategies and its exploitation for crop improvement, especially in the context of F1 hybrid breeding. Scope: We review the literature on SI and SC in outcrossing grass species. We review the currently available genomic tools and approaches used to discover and characterize novel SC sources. We discuss opportunities barely explored for outcrossing grasses that SC facilitates. Specifically, we discuss strategies for wide SC introgression in the context of the Lolium-Festuca complex and the use of SC to develop immortalized mapping populations for the dissection of a ... Presentar Todo |
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Palabras claves : |
F 1hybrid breeding; Heterosis; Inbreeding; Inbreeding depression; Introgression; Poaceae; Self-compatibility; Self-incompatibility. |
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Asunto categoría : |
-- |
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URL : |
https://academic.oup.com/aob/article-pdf/127/7/841/38823254/mcab043.pdf
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Marc : |
LEADER 03586naa a2200325 a 4500
001 1062318
005 2021-07-22
008 2021 bl uuuu u00u1 u #d
022 $a0305-7364
024 7 $a10.1093/aob/mcab043$2DOI
100 1 $aCROPANO, C.
245 $aCharacterization and practical use of self-compatibility in outcrossing grass species.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 25 November 2020, Revision received 12 March 2021, Editorial decision 17 March 2021, Accepted 18 March 2021, Published 23 March 2021, Corrected and typeset 24 June 2021. Corresponding author: Thorogood, D.; Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, United Kingdom; email:dnt@aber.ac.uk
520 $aABSTRACT - Background: Self-incompatibility (SI) systems prevent self-fertilization in several species of Poaceae, many of which are economically important forage, bioenergy and turf grasses. Self-incompatibility ensures cross-pollination and genetic diversity but restricts the ability to fix useful genetic variation. In most inbred crops it is possible to develop high-performing homozygous parental lines by self-pollination, which then enables the creation of F1 hybrid varieties with higher performance, a phenomenon known as heterosis. The inability to fully exploit heterosis in outcrossing grasses is partially responsible for lower levels of improvement in breeding programmes compared with inbred crops. However, SI can be overcome in forage grasses to create self-compatible populations. This is generating interest in understanding the genetical basis of self-compatibility (SC), its significance for reproductive strategies and its exploitation for crop improvement, especially in the context of F1 hybrid breeding. Scope: We review the literature on SI and SC in outcrossing grass species. We review the currently available genomic tools and approaches used to discover and characterize novel SC sources. We discuss opportunities barely explored for outcrossing grasses that SC facilitates. Specifically, we discuss strategies for wide SC introgression in the context of the Lolium-Festuca complex and the use of SC to develop immortalized mapping populations for the dissection of a wide range of agronomically important traits. The germplasm available is a valuable practical resource and will aid understanding the basis of inbreeding depression and hybrid vigour in key temperate forage grass species. Conclusions: A better understanding of the genetic control of additional SC loci offers new insight into SI systems, their evolutionary origins and their reproductive significance. Heterozygous outcrossing grass species that can be readily selfed facilitate studies of heterosis. Moreover, SC introduction into a range of grass species will enable heterosis to be exploited in innovative ways in genetic improvement programmes. © 2021 The Author(s) 2021. Published by Oxford University Press on behalf of the Annals of Botany Company.
653 $aF 1hybrid breeding
653 $aHeterosis
653 $aInbreeding
653 $aInbreeding depression
653 $aIntrogression
653 $aPoaceae
653 $aSelf-compatibility
653 $aSelf-incompatibility
700 1 $aPLACE, I.
700 1 $aMANZANARES, C.
700 1 $aDO CANTO, J.
700 1 $aLÜBBERSTEDT, T.
700 1 $aSTUDER, B.
700 1 $aTHOROGOOD, D.
773 $tAnnals of Botany, June 2021, volume 127, Issue 7, Pages 841 - 8524. OPEN ACCESS. Doi: https://doi.org/10.1093/aob/mcab043
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