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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
22/01/2021 |
Actualizado : |
14/04/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
DOI : |
10.1016/j.vas.2020.100163 |
Idioma : |
Inglés |
Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
Asunto categoría : |
L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
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Marc : |
LEADER 02681naa a2200373 a 4500 001 1061678 005 2021-04-14 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1016/j.vas.2020.100163$2DOI 100 1 $aDELPIAZZO, R. 245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource] 260 $c2021 500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy 520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. 653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS 653 $aCAMPYLOBACTER FETUS 653 $aMOLECULAR DIAGNOSIS 653 $aMOLECULAR DIAGNOSTICS 653 $aQPCR 700 1 $aBARCELLOS, M. 700 1 $aBARROS, S. 700 1 $aBENTANCOR, L. 700 1 $aFRAGA, M. 700 1 $aGIL, J. 700 1 $aIRAOLA, G. 700 1 $aMORSELLA, C. 700 1 $aPAOLICCHI, F. 700 1 $aPÉREZ, R. 700 1 $aRIET-CORREA, F. 700 1 $aSANGUINETTI, M. 700 1 $aSILVA, A. 700 1 $aSILVEIRA, C.S. 700 1 $aCALLEROS, L. 773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
02/06/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
REINA, L.; BOTTINI, G.; BENNADJI, Z.; VINCIGUERRA, V.; FERREIRA CHIESA, F.A.; MENÉNDEZ, P.; MOYNA, G. |
Afiliación : |
LUIS REINA, Universidad de la República (UdelaR)/ Facultad de Química.; GUALBERTO BOTTINI, cDepartamento de Química del Litoral, CENUR Litoral Norte, UdelaR, Paysandú, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VITTORIO VINCIGUERRA, Dipartimento per la Innovazione nei Sistemi Biologici, Agroalimentari e Forestali, Università della Tuscia, Largo dell’Università, Italy; FERNANDO AMAURY FERREIRA CHIESA, Universidad de la República (UdelaR)/ Facultad de Química.; PILAR MENÉNDEZ, Universidad de la República (UdelaR)/ Facultad de Química.; GUILLERMO MOYNA, c Departamento de Química del Litoral, CENUR Litoral Norte, UdelaR, Paysandú, Uruguay. |
Título : |
Aggregation Behavior of 6-Isocassine and N-Methyl-6-Isocassine: Insights into the Biological Mode of Action of Lipid Alkaloids. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Natural Product Communications, 2016, Volume 11, Issue 11, Pages 1641-1644. DOI. https://doi.org/10.1177/1934578X1601101104 |
DOI : |
10.1177/1934578X1601101104 |
Idioma : |
Inglés |
Notas : |
Article history: Received: February 1st, 2016// Accepted: May 25th, 2016. Dedicated to the memory of our esteemed colleague and friend Eduardo Alonso Paz. Acknowledgments - Funding from the INIA (award L4-FO-21-0-00), the ANII (award POS_NAC_2014_1_102226), the DGRC-UdelaR (Programa 720), and the PEDECIBA is greatly acknowledged. |
Contenido : |
The aggregation behavior of 6-isocassine and N-methyl-6-isocassine, two piperidin-3-ol alkaloids isolated respectively from the barks of Prosopis nigra and
P. affinis, was investigated using a combination of NOE experiments and diffusion measurements in solvents of varying polarity and hydrogen bonding
capacity. While the NOE enhancements for N-methyl-6-isocassine are positive, regardless of the solvent, those for 6-isocassine shift from negative to positive
when going from chloroform-d to methanol-d4 solution. In addition, despite the self-diffusion coefficients of both compounds being virtually identical in
methanol-d4, N-methyl-6-isocassine diffuses nearly twice as fast as the non-methylated alkaloid in chloroform-d. The changes in rotational and translational
dynamics observed between solvents for 6-isocassine suggest that the molecule forms dimeric head-to-head aggregates in non-polar aprotic environments, a
behavior that could help explain the biological mode of action that has been proposed for this type of alkaloids. |
Palabras claves : |
AGGREGATION; DIFFUSION; HYDROGEN BONDING; NOE ENHANCEMENTS; PIPERIDINE ALKALOIDS. |
Asunto categoría : |
K10 Producción forestal |
Marc : |
LEADER 02269naa a2200277 a 4500 001 1016936 005 2020-06-02 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1177/1934578X1601101104$2DOI 100 1 $aREINA, L. 245 $aAggregation Behavior of 6-Isocassine and N-Methyl-6-Isocassine$bInsights into the Biological Mode of Action of Lipid Alkaloids.$h[electronic resource] 260 $c2016 500 $aArticle history: Received: February 1st, 2016// Accepted: May 25th, 2016. Dedicated to the memory of our esteemed colleague and friend Eduardo Alonso Paz. Acknowledgments - Funding from the INIA (award L4-FO-21-0-00), the ANII (award POS_NAC_2014_1_102226), the DGRC-UdelaR (Programa 720), and the PEDECIBA is greatly acknowledged. 520 $aThe aggregation behavior of 6-isocassine and N-methyl-6-isocassine, two piperidin-3-ol alkaloids isolated respectively from the barks of Prosopis nigra and P. affinis, was investigated using a combination of NOE experiments and diffusion measurements in solvents of varying polarity and hydrogen bonding capacity. While the NOE enhancements for N-methyl-6-isocassine are positive, regardless of the solvent, those for 6-isocassine shift from negative to positive when going from chloroform-d to methanol-d4 solution. In addition, despite the self-diffusion coefficients of both compounds being virtually identical in methanol-d4, N-methyl-6-isocassine diffuses nearly twice as fast as the non-methylated alkaloid in chloroform-d. The changes in rotational and translational dynamics observed between solvents for 6-isocassine suggest that the molecule forms dimeric head-to-head aggregates in non-polar aprotic environments, a behavior that could help explain the biological mode of action that has been proposed for this type of alkaloids. 653 $aAGGREGATION 653 $aDIFFUSION 653 $aHYDROGEN BONDING 653 $aNOE ENHANCEMENTS 653 $aPIPERIDINE ALKALOIDS 700 1 $aBOTTINI, G. 700 1 $aBENNADJI, Z. 700 1 $aVINCIGUERRA, V. 700 1 $aFERREIRA CHIESA, F.A. 700 1 $aMENÉNDEZ, P. 700 1 $aMOYNA, G. 773 $tNatural Product Communications, 2016, Volume 11, Issue 11, Pages 1641-1644. DOI. https://doi.org/10.1177/1934578X1601101104
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