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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
26/09/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ABREO, E.; MARTINEZ, S.; BETTUCCI, L.; LUPO, S. |
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Afiliación : |
SEBASTIAN MARTINEZ KOPP, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Phaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Phytopathologia Mediterranea. 2011. v.50 (Supplement), p.77-85. |
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ISSN : |
1593-2095 |
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DOI : |
10.14601/Phytopathol_Mediterr-8682 |
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Idioma : |
Inglés |
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Notas : |
Article history: accepted for publication: February 15, 2010; published 2011.
http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682 |
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Contenido : |
Summary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one
of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines. MenosSummary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other ... Presentar Todo |
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Palabras claves : |
ASYMPTOMATIC CANES; ESCA; MONITORING; PETRI DISEASE; SPECIFIC PRIMERS. |
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Thesagro : |
ENFERMEDADES DE LA VID; ENFERMEDADES DE LAS PLANTAS; URUGUAY. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02850naa a2200289 a 4500
001 1050566
005 2019-10-11
008 2011 bl uuuu u00u1 u #d
022 $a1593-2095
024 7 $a10.14601/Phytopathol_Mediterr-8682$2DOI
100 1 $aABREO, E.
245 $aPhaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay.$h[electronic resource]
260 $c2011
500 $aArticle history: accepted for publication: February 15, 2010; published 2011. http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682
520 $aSummary Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected. Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines.
650 $aENFERMEDADES DE LA VID
650 $aENFERMEDADES DE LAS PLANTAS
650 $aURUGUAY
653 $aASYMPTOMATIC CANES
653 $aESCA
653 $aMONITORING
653 $aPETRI DISEASE
653 $aSPECIFIC PRIMERS
700 1 $aMARTINEZ, S.
700 1 $aBETTUCCI, L.
700 1 $aLUPO, S.
773 $tPhytopathologia Mediterranea. 2011.$gv.50 (Supplement), p.77-85.
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| 15. |  | SAN ROMÁN, L.; DEL RÍO, A.; HORNOS, M.; RAVERA, M. Monitoreo de Thaumastocoris peregrinus, antecedentes, situación actual y resultados en Forestal Oriental. ln: INIA TACUAREMBÓ. Seminario Técnico, 26 de noviembre, Tacuarembó, 2009. Sanidad forestal. Tacuarembó (Uruguay): INIA, 2009. p. 14-19 (INIA Serie Actividades de Difusión ; 594)| Biblioteca(s): INIA Tacuarembó. |
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| 17. | | MARTÍNEZ, R.S.; ROMÁN, L.; ROMAN, L.; PLA, M.; MAZZINO, E.; PALLADINO, R.A.; LA MANNA, A. Efecto del uso de medidas de mitigación del estrés térmico sobre el consumo y comportamiento de vacas Holstein en lactancia avanzada. Comunicación. NA 2, Nutrición y Alimentación Animal. IN: REVISTA ARGENTINA DE PRODUCCIÓN ANIMAL, 2016, VOL. 36, ISS.1 (SUPL). 39 CONGRESO ARGENTINO DE PRODUCCIÓN ANIMAL. 19-21 OCTUBRE 2016. ACTAS. Buenos Aires (Argentina): AAPA. p. 190.| Tipo: Trabajos en Congresos/Conferencias |
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| 20. | | ROMAN, L.; BANCHERO, G.; MORALES-PIÑEYRUA, J.; ACOSTA, Y.; MENDOZA, A.; PLA, M.; LA MANNA, A. Acceso a sombra artificial durante el periodo seco en vacas lecheras: 2. Desempeño productivo. [Effect of access to artificial shade during the dry period in dairy cow. 2. Productive performance]. SP 77, In: Congreso Argentino de Producción Animal, 37., 2014. Resúmenes. Joint; Meeting American Society of Animal Science, 2nd.; Congreso Sociedad Chilena de Producción , 39. Buenos Aires 20-22 de octubre. AR: ASAS/AAPA, 2014. p. 289. (Revista Argentina de Producción Animal; 2014; 34; supl.1).| Tipo: Abstracts/Resúmenes |
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