03652naa a2200385 a 450000100080000000500110000800800410001902200140006002400360007410000150011024501800012526000090030550006330031452018360094765300340278365300330281765300080285065300160285865300310287465300210290565300360292665300410296265300250300370000130302870000150304170000150305670000170307170000180308870000170310670000140312370000170313770000170315470000180317177300770318910644792024-02-28 2024 bl uuuu u00u1 u #d a1519-566X7 a10.1007/s13744-024-01128-72DOI1 aABBATE, S. aBt soybean Cry1Ac does not affect development, reproduction, or feeding behavior of red-banded stink bug Piezodorus guildinii (HemipterabPentatomidae).h[electronic resource] c2024 aArticle history: Received 17 March 2023; Accepted 4 January 2024; Published 25 January 2024. -- Correspondence: Abbate, S.; Est. Exp. "Dr. Mario A. Cassinoni", Department of Plant Protection, Polo Agroalimentario y Agroindustrial Paysandú, Centro Universitario Regional Litoral Norte, Universidad de La República, Paysandú, Uruguay; email:abbate@fagro.edu.uy -- Funding: Funding This research was part of a scholarship from Agencia Nacional de Investigación e Innovación ANII-Uruguay (POS_NAC_2011_1_3559) and Comisión Académica de Posgrado CAPUniversidad de la República, Uruguay to SA. -- Edited by Marcos R de Faria. aABSTRACT.- Bt soybean cultivation is increasing worldwide. The Cry1Ac protein expressed in Bt soybean efficiently controls several lepidopteran pests. The stink bug, Piezodorus guildinii (Westwood), a major pest for soybean in the Americas, is not controlled by Bt crops, although possible sub-lethal effects may occur. Even if there were no negative effects for sting bug, ingesting toxins could affect its bio-controllers. We tested through ELISA detection if P. guildinii ingests Cry1Ac from Bt soybean and possible effects on its development, reproduction, survival, and feeding behavior. Biological traits were evaluated under controlled conditions of nymphs and adults feeding on pods of near-isogenic cultivars DM5958iPRO (Bt) and DM59i (non-Bt). Feeding behavior was recorded using an AC-DC electropenetrography (EPG) device. Results indicated that P. guildinii ingested the Cry1Ac protein; however, nymphal period and accumulated survival percentage did not differ between cultivars. Feeding on Bt soybean pods did not affect fecundity (i.e., number of egg masses and eggs/female) nor egg viability. Different feeding behaviors were only detected on the pathway phase (stylet penetration into plant tissue), which was more pronounced in the Bt cultivar. However, the total duration of the feeding activities on seeds was numerically higher (ca. 2X) on Bt plants compared to non-Bt. This is the first study to demonstrate that P. guildinii does ingest the Cry1Ac protein and excrete it without being absorbed, probably explaining the lack of direct adverse effects on its biological parameters. EPG could indicate that Bt soybean plants might be less palatable than non-Bt to red-banded stink bug. © 2024, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. aBt genetically modified crops aElectrical penetration graph aEPG aGlycine max aIntegrated pest management aNon-target pests aPLATAFORMA DE BIOINSUMOS - INIA aSustainable Development Goals (SDGs) aZero hunger - Goal 21 aPONS, X.1 aALTIER, N.1 aLUCINI, T.1 aBONANSEA, S.1 aBENTANCUR, O.1 aPILÓN, A.A.1 aSILVA, H.1 aDE SOUZA, G.1 aIRIGOYEN, L.1 aPANIZZI, A.R. tNeotropical Entomology. 2024. https://doi.org/10.1007/s13744-024-01128-7