03328naa a2200301 a 450000100080000000500110000800800410001902200200006002400310008010000170011124501160012826000090024450013050025352012010155865300100275965300100276965300150277965300090279465300380280365300110284165300090285270000130286170000150287470000190288970000180290870000170292677300830294310644662024-02-20       2024    bl uuuu     u00u1 u    #d  aeISSN 2073-44257 a10.3390/genes150202292DOI1 aROMANIUK, E.  aIdentification of candidate genes and pathways linked to the temperament trait in sheep.h[electronic resource]  c2024  aArticle history: Submission received: 4 December 2023; Revised: 5 January 2024;  Accepted: 10 January 2024; Published: 11 February 2024. -- Academic Editor: Emilia Bagnicka. -- Funding: This research was funded by the Comisión Sectorial de Investigación Científica (CSIC, Universidad de la República), which approved the project CSIC_I+D_2018_287; the SMARTER project funded by the European Union Horizon 2020 program (agreement no. 772787); the projects INIA CL 40 (resistance to parasites) and CL 38 (RUMIAR); and to the Comité Académico de Posgrados for the scholarship master?s degree 2020 (POS_NAC_2019_1_157979), Universidad de la República. -- Institutional Review Board Statement: The animal study protocol was approved by the Honorary Commission for Animal Experimentation (Comisión Honoraria de Experimentación Animal, CHEA, protocols N° 021130-006307-11 and 020300-000653-18-ID, CHEA 701), Universidad de la República. -- This article belongs to the Section Animal Genetics and Genomics (https://www.mdpi.com/journal/genes/sections/Animal_Genetics_Genomics ) -- Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).  aABSTRACT.- Temperament can be defined as the emotional variability among animals of the same species in response to the same stimulus, grouping animals by their reactivity as nervous, intermediate, or calm. Our goal was to identify genomic regions with the temperament phenotype measured by the Isolation Box Test (IBT) by single-step genome-wide association studies (ssGWAS). The database consisted of 4317 animals with temperament records, and 1697 genotyped animals with 38,268 effective Single Nucleotide Polymorphism (SNP) after quality control. We identified three genomic regions that explained the greatest percentage of the genetic variance, resulting in 25 SNP associated with candidate genes on chromosomes 6, 10, and 21. A total of nine candidate genes are reported for the temperament trait, which is: PYGM, SYVN1, CAPN1, FADS1, SYT7, GRID2, GPRIN3, EEF1A1 and FRY, linked to the energetic activity of the organism, synaptic transmission, meat tenderness, and calcium associated activities. This is the first study to identify these genetic variants associated with temperament in sheep, which could be used as molecular markers in future behavioral research. © 2024 by the authors.  aCAPN1  aGRID2  aOvis aries  aPYMG  aSISTEMA GANADERO EXTENSIVO - INIA  aSsGWAS  aSYT71 aVERA, B.1 aPERAZA, P.1 aCIAPPESONI, G.1 aDAMIÁN, J.P.1 aVAN LIER, E.  tGenes. 2024; 15(2):229. https://doi.org/10.3390/genes15020229  -- OPEN ACCESS.