01965naa a2200229 a 450000100080000000500110000800800410001902400380006010000240009824501020012226000090022450001540023352010560038765300170144365300280146065300160148870000190150470000170152370000210154070000170156177301570157810642092023-06-28       2022    bl uuuu     u00u1 u    #d7 a10.1007/978-3-030-82381-8_252DOI1 aDE TRAVENSOLO, R.F.  aMicroarrays application in life sciencesbThe beginning of the revolution.h[electronic resource]  c2022  aCorrespondence: Carrilho, E.; Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; email:emanuel@iqsc.usp.br --  aPrevious to Next Generation Sequencing (NGS), a different technology allowed science to drive through the roads of genetic analysis, the microarray technology. Shortly, microarrays consist of a grid of spots, with each spot containing single-stranded DNA sequences attached to a solid surface. For microarray analysis, the mRNA must be extracted from the sample and converted to a labeled cDNA strand, and later added to the microarray device. The DNA sequences on the microchip are fragments of genes of interest, and once the sample is added to the microarray, the cDNA strands from the sample hybridize with the strands fixed on the device if they are matching. In that way, a single device allows the analyst to check for the presence of several genes at once. In this chapter, the reader will learn precisely how microarray analysis works, concepts to produce in-lab microarrays, and the main research areas applying the microarray technology. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. All rights reseverd.  aDNA analysis  aMicroarray applications  aMicroarrays1 aFERREIRA, V.G.1 aFEDERICI, M.1 aDE LEMOS, E.G.M.1 aCARRILHO, E.  tIn: L. T. Kubota et al. (eds.). 2022. Tools and Trends in Bioanalytical Chemistry. Springer. Pages 483-496. https://doi.org/10.1007/978-3-030-82381-8_25