02982naa a2200361 a 450000100080000000500110000800800410001902200240006002400350008410000180011924501300013726000090026750004410027652014200071765300290213765300150216665300090218165300390219065300140222965300560224365300170229970000150231670000270233170000150235870000170237370000150239070000160240570000140242170000170243570000180245270000150247077301350248510641882023-09-01       2023    bl uuuu     u00u1 u    #d  a1943-4936 (online).7 a10.1177/104063872311768162DOI1 aGIANNITTI, F.  aCanine leproid granuloma caused by a member of the Mycobacterium tuberculosis complex. (Brief Report).h[electronic resource]  c2023  aArticle history: First published online May 19, 2023; Issue published July 2023. -- Corresponding author: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50, km 11 (70006), La Estanzuela, Colonia, Uruguay. fgiannitti@inia.org.uy -- Funding: Funded by research grant PL_27 N-23398 from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay.  aABSTRACT.- Canine leproid granuloma (CLG) is a chronic form of dermatitis that has been associated with nontuberculous mycobacterial infections in Africa, Oceania, the Americas, and Europe. We report here a case of CLG associated with a member of the Mycobacterium tuberculosis complex (MTBC), which could be of public health concern. An 8-y-old pet dog developed 0.5-1-cm diameter, raised, firm, nonpruritic, alopecic, painless skin nodules on the external aspects of both pinnae. Histologic examination revealed severe pyogranulomatous dermatitis with intracellular Ziehl-Neelsen-positive bacilli that were immunoreactive by immunohistochemistry using a polyclonal primary antibody that recognizes tuberculous and nontuberculous Mycobacterium species. DNA extracted from formalin-fixed, paraffin-embedded skin sections was tested by a Mycobacterium genus-specific nested PCR assay targeting the 16S rRNA gene. BLAST sequence analysis of 214-bp and 178-bp amplicons showed 99.5% identity with members of the MTBC; however, the agent could not be identified at the species level. Although CLG has been associated traditionally with nontuberculous mycobacterial infections, the role of Mycobacterium spp. within the MTBC as a cause of this condition, and the role of dogs with CLG as possible sources of MTBC to other animals and humans, should not be disregarded given its zoonotic potential. © 2023 The Author(s).  aCanine leproid granuloma  aDermatitis  aDogs  aMycobacterium tuberculosis complex  aPathology  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA  aPyogranuloma1 aDORSCH, M.1 aFERNÁNDEZ-CIGANDA, S.1 aRABAZA, A.1 aVÁZQUEZ, S.1 aCÉSAR, C.1 aHURTADO, J.1 aGREIF, G.1 aRABENECK, D.1 aBHATNAGAR, J.1 aRITTER, J.  tJournal of Veterinary Diagnostic Investigation. 2023, Volume 35, Issue 4, pages 439-443. https://doi.org/10.1177/10406387231176816