02839nam a2200265 a 450000100080000000500110000800800410001902200140006002400360007410000170011024501150012726001110024250005480035352014070090165300650230865300240237365300300239765300230242765300170245065300380246765300150250570000160252070000160253670000210255210640652023-05-02       2023    bl uuuu     u01u1 u    #d  a1380-37437 a10.1007/s11032-023-01371-72DOI1 aBARAIBAR, S.  aValidation of SNP markers for selection of semi-dwarf and peduncle extrusion in barley.h[electronic resource]  aMolecular Breeding, 2023, Volume 43, Issue 4, Article 30. https://doi.org/10.1007/s11032-023-01371-7c1007  aArticle history: Received 16 May 2022; Accepted 16 March 2023; Published online 13 April 2023. -- Corresponding author: Bonnecarrère, V.; Instituto Nacional de Investigación Agropecuaria (INIA), Area Mejoramiento Genetico y Biotecnologia Vegetal, Estación Experimental INIA Las Brujas, Ruta 48 km 10, Canelones, Uruguay; email:vbonnecarrere@lb.inia.org.uy  -- FUNDING: This work was funded by the INIA (Instituto Nacional de Investigación Agropecuaria) Projects: Mejoramiento de cultivo de secano and Mejoramiento molecular de cultivos. --  aThis study aimed to validate the use of two SNP markers associated to a sdw1 allele identified previously in a short barley genotype (ND23049) with an adequate peduncle extrusion which reduces predisposition to fungal disease development. First, the GBS SNP were converted in a KASP marker but only one of them, named TP4712, correctly amplified all allelic variations and had a Mendelian segregation in a F2 population. To corroborate the association between TP4712 allele with plant height and peduncle extrusion, a total of 1221 genotypes were genotyped and evaluated for both traits. Out of the 1221 genotypes, 199 were F4 lines, 79 were a diverse panel, and 943 were two complete breeding cohorts of stage 1 yield trials. To corroborate the association between the sdw1 allele and the short plant height with adequate peduncle extrusion, contingency tables were created, grouping the 2427 data points into categories. Based on the contingency analysis, it was demonstrated that the higher proportion of short plants with adequate peduncle extrusion were present in genotypes carrying the SNP allele of ND23049 regardless the population and the sowing date. This study develops a marker-assisted selection tool to accelerate the introgression of favourable alleles for plant height and peduncle extrusion in adapted germplasm. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.  aÁREA MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA  aBreeding population  aMarker-assisted selection  aPeduncle extrusion  aPlant height  aSISTEMA AGRÍCOLA-GANADERO - INIA  aSNP marker1 aIRIARTE, W.1 aDE LEON, W.1 aBONNECARRERE, V.