02723naa a2200313 a 450000100080000000500110000800800410001902200140006002400350007410000150010924501510012426000090027550006590028452010580094365300240200165300110202565300150203665300100205165300490206165300120211070000280212270000210215070000120217170000270218370000260221070000150223670000170225177301410226810637132022-11-08       2022    bl uuuu     u00u1 u    #d  a2297-17697 a10.3389/fvets.2022.9902282DOI1 aPARODI, P.  aCharacterization of strains of Anaplasma marginale from clinical cases in bovine using major surface protein 1a in Uruguay.h[electronic resource]  c2022  aArticle history: Received 09 July 2022; Accepted 30 August 2022; Published 20 September 2022. -- Correspondence author:  Parodi, P.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Salud Animal, Estación experimental INIA Tacuarembó, Tacuarembó, Uruguay; email:pparodi@inia.org.uy -- This article is part of the Research Topic "Current Knowledge In Control And Immunoprophylaxis Of Tick-Borne Diseases Of Veterinary Importance: Special Focus In Diseases Of Cattle" (https://www.frontiersin.org/research-topics/37164/current-knowledge-in-control-and-immunoprophylaxis-of-tick-borne-diseases-of-veterinary-importance-s#articles )  aABSTRACT.- The major surface protein 1a (MSP1a) gene has been used to characterize Anaplasma marginale genetic diversity. This pathogen causes significant productivity and economic losses to the cattle industry. The objective of the present study was to report the first characterization of A. marginale genetic diversity in Uruguay based on MSP1a genotypes and their putative relationship with Rhipicephalus microplus. This cross-sectional study was conducted between 2016 and 2020. The study included whole blood samples from clinical cases of bovine anaplasmosis obtained from 30 outbreaks located in six Uruguay territorial departments. Diagnosis was performed using Giemsa-stained smears and confirmed by nested Polymerase Chance Reaction (nPCR) targeting the A. marginale major surface protein 5 gene. The genetic diversity of A. marginale strains was characterized by analyzing the microsatellite and tandem repeats of MSP1a.  Copyright © 2022 Parodi, Armúa-Fernández, Schanzembach, Mir, Benítez-Galeano, Rodríguez-Osorio, Rivero and Venzal.  aAnaplasma marginale  aBovine  aGenotyping  aMSP1a  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL  aUruguay1 aARMÚA-FERNÁNDEZ, M.T.1 aSCHANZEMBACH, M.1 aMIR, D.1 aBENÍTEZ-GALEANO, M.J.1 aRODRÍGUEZ-OSORIO, N.1 aRIVERO, R.1 aVENZAL, J.M.  tFrontiers in Veterinary Science, September 2022, Volume 920, Article 990228. OPEN ACCESS. doi: https://doi.org/10.3389/fvets.2022.990228