02775naa a2200325 a 450000100080000000500110000800800410001902200140006002400360007410000150011024501700012526000090029550004600030452013220076465300090208665300180209565300140211365300320212765300080215970000150216770000140218270000150219670000160221170000170222770000150224470000150225970000170227470000180229177301400230910635662022-12-01       2022    bl uuuu     u00u1 u    #d  a0165-73807 a10.1007/s11259-022-09983-82DOI1 aVASINI, B.  aPhylogenetic and multiple-locus variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina.h[electronic resource]  c2022  aArticle history: Received 24 February 2022; Accepted 3 August 2022. -- Corresponding author: Vasini, B.; Laboratorio de Bacteriología-Grupo de Sanidad Animal, Unidad Integrada INTA-Universidad Nacional de Mar del Plata-Balcarce, Buenos Aires, Argentina; email:vasinibrenda@gmail.com --  Founding: This study was funded by ANPCyT (PICT grant, project PICT2015-1541), CONICET (PIP grant, project PIP11220150100316CO) and INTA (project 2019-PD-E5-I103-001).  aABSTRACT.- Paratuberculosis is a worldwide chronic enteric disease of ruminants, caused by Mycobacterium avium subsp. paratuberculosis (MAP). While MAP has been widely investigated all around the world, little is known about the different strains that circulate in each country. This study describes the genetic diversity of MAP isolates from different bovine and deer herds from Argentina, analyzed by Multiple-Locus Variable number tandem repeat Analysis (MLVA), as well as the phylogenetic relatedness between geographically distant isolates through Whole Genome Sequencing (WGS) and core-genome analysis. A total of 90 MAP isolates were analyzed. The results showed seven different MLVA genotypes, with almost 75% of them belonging to pattern INMV 1, described in all the herds studied. WGS results suggested the presence of a common INMV 1 strain circulating throughout the country. Our results allow confirming the coexistence of different strains in time and space and the mixed infections identified in some animals. These observations suggest the absence of animal monitoring prior to introduction to the herds and the need for a control program in the country. This study represents the first to report WGS of MAP strains in Argentina. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.  aMLVA  aMycobacterium  aRuminants  aUNIDAD MIXTA PASTEUR + INIA  aWGS1 aFARACE, P.1 aARIEL, A.1 aCIRONE, K.1 aMÉNDEZ, L.1 aMORSELLA, C.1 aFRESIA, P.1 aIRAOLA, G.1 aGIOFFRÉ, A.1 aPAOLICCHI, F.  tVeterinary Research Communications, December 2022, Volume 46, Issue 4, pages 1121-1129. doi: https://doi.org/10.1007/s11259-022-09983-8