03303naa a2200325 a 450000100080000000500110000800800410001902400350006010000140009524501200010926000090022950004810023852018620071965300300258165300270261165300380263865300150267665300140269165300230270565300100272870000140273870000180275270000130277070000140278370000120279770000140280970000140282370000150283777301250285210628702022-09-02       2022    bl uuuu     u00u1 u    #d7 a10.1093/g3journal/jkac0642DOI1 aSILVA, P.  aApplied phenomics and genomics for improving barley yellow dwarf resistance in winter wheat.h[electronic resource]  c2022  aArticle history: Received: 22 December 2021/Accepted: 12 March 2022/Published: 30 March 2022.  The Author(s) (2022) . Published by Oxford University Press on behalf of the Genetics Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.  aAbstract: Barley yellow dwarf (BYD) is one of the major viral diseases of cereals. Phenotyping BYD in wheat is extremely challenging due to similarities to other biotic and abiotic stresses. Breeding for resistance is additionally challenging as the wheat primary germplasm pool lacks genetic resistance, with most of the few resistance genes named to date originating from a wild relative species. The objectives of this study were to, i) evaluate the use of high-throughput phenotyping (HTP) from unmanned aerial systems to improve BYD assessment and selection, ii) identify genomic regions associated with BYD resistance, and iii) evaluate genomic prediction models ability to predict BYD resistance. Up to 107 wheat lines were phenotyped during each of five field seasons under both insecticide treated and untreated plots. Across all seasons, BYD severity was lower with the insecticide treatment and plant height (PTHTM) and grain yield (GY) showed increased values relative to untreated entries. Only 9.2% of the lines were positive for the presence of the translocated segment carrying resistance gene Bdv2 on chromosome 7DL. Despite the low frequency, this region was identified through association mapping. Furthermore, we mapped a potentially novel genomic region for resistance on chromosome 5AS. Given the variable heritability of the trait (0.211 ? 0.806), we obtained relatively good predictive ability for BYD severity ranging between 0.06 ? 0.26. Including Bdv2 on the predictive model had a large effect for predicting BYD but almost no effect for PTHTM and GY. This study was the first attempt to characterize BYD using field-HTP and apply GS to predict the disease severity. These methods have the potential to improve BYD characterization and identifying new sources of resistance will be crucial for delivering BYD resistant germplasm.  aBarley yellow dwarf (BYD)  aGenomic Selection (GS)  aHigh-throughput Phenotyping (HTP)  aResistance  aTolerance  aTriticum  aestivum  aVirus1 aEVERS, B.1 aKIEFFABER, A.1 aWANG, X.1 aBROWN, R.1 aGAO, L.1 aFRITZ, A.1 aCRAIN, J.1 aPOLAND, J.  tG3 Genes| Genomes| Genetics, (Bethesda, Md.), 2022;, jkac064, Open Access. DOI:https://doi.org/10.1093/g3journal/jkac064