03034naa a2200265 a 450000100080000000500110000800800410001902200140006002400360007410000190011024501510012926000090028050006680028952015330095765300150249065300110250565300190251665300310253565300280256665300160259465300100261070000140262070000200263477301140265410628272022-03-14       2022    bl uuuu     u00u1 u    #d  a0168-81627 a10.1007/s10493-022-00691-12DOI1 aMIRABALLES, C.  aEvaluation of the one-side tick counting technique and of the level of infestation of bovines with Rhipicephalus microplus.h[electronic resource]  c2022  aArticle history: Received: 12 December 2020; Accepted: 18 January 2022. Corresponding author:  Riet-Correa, F.; Programa de pós-graduação em Ciência Animal nos Trópicos, Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Salvador, Brazil; email:franklinrietcorrea@gmail.com  --  Funding text: We would like to acknowledge Dr. Javier Sanchez and Dr. Henrik Stryhn for collaborating with the data analysis and Tatiana Saporitti for performing the acaricide resistance tests. The research presented in this publication was supported by funds from the National Agency for Research and Innovation (ANII) under code MOV_IDRC_2018_2_153357.  aABSTRACT. -  Counting ticks on one side of a bovine has been widely used to estimate the overall number of ticks; however, the accuracy of this method has not been determined. This work aimed to evaluate the one-side technique for counting ticks, identify bovines with a higher level of infestation, and determine the prevalence of infestation of adults and calves. To evaluate the one-side technique, ticks were counted on both sides of 352 bovines from three farms in nine months, and total numbers were correlated with the numbers counted on the left sides. The intraclass correlation coefficients reached values >0.99 for all farms, independent of the season. These results demonstrated that counting ticks on one-side and multiplying by 2 is a reliable method for estimating the overall number of ticks. To evaluate the level of infestation of various bovines, ticks were counted monthly, for 12 months, on the same bovines from seven farms. An animal was considered highly infested if the Anscombe residuals from a negative binomial GLM were ≥4, at least 2×. The number of bovines with infestation levels higher than expected varied among farms from zero to 3. Using this method, it is possible to evaluate the most infested bovines in order to remove them from the herd. The prevalence of infestation in calves reached 100% in five of the seven farms and 3- to 15-month-old calves had significantly higher infestation than adults.  © 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.  aAcaricides  aCattle  aCounting ticks  aIntegrated pest management  aPLATAFORMA SALUD ANIMAL  aTick burden  aTicks1 aTAÑO, M.1 aRIET-CORREA, F.  tExperimental and Applied Acarology, 2022. [Article In Press]. doi: https://doi.org/10.1007/s10493-022-00691-1