02502naa a2200241 a 450000100080000000500110000800800410001902400390006010000160009924502020011526000090031750004930032652011690081965300310198865300220201965300240204165300230206565300360208870000160212470000170214070000160215777300870217310623222021-07-23       2021    bl uuuu     u00u1 u    #d7 a10.1080/14786419.2021.19488442DOI1 aOLIVARO, C.  aChemical characterisation and in vitro anthelmintic activity of phenolic-rich extracts from the leaves and branches of Maytenus ilicifolia, a native plant from South America.h[electronic resource]  c2021  aArticle history: Received 28 Mar 2021, Accepted 20 Jun 2021, Published online: 06 Jul 2021. Correspondence: Cristina Olivaro, emai: cristina.olivaro@cut.edu.uy   -      Supplemental data for this article can be accessed online at https://www.tandfonline.com/doi/suppl/10.1080/14786419.2021.1948844 This work was supported by the INIA under Grant INIA CL_40 and PEDECIBA. Instituto Nacional de Investigación Agropecuaria (INIA); Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).  aABSTRACT - Free (epi) catechin, quercetin, (epi) gallocatechin, flavonol glycosides and condensed tannins were identified according to their molecular mass, characteristic product ions and retention times in extracts obtained from leaves and branches of Maytenus ilicifolia (Congorosa) by mass spectrometry. The in vitro anthelmintic activity against cattle gastrointestinal nematodes of Congorosa extract was determined using the Egg Hatch Inhibition Assay. Additionally, commercial quercetin, gallocatechin and epicatechin were evaluated. Although total phenolics, total tannins and condensed tannins contents were lower in branches extract than in leaves extract, the EC50 were 0.065mg/mL and 0.890 mg/mL for branches and leaves extract, respectively. Moreover, the use of polyvinylpyrrolidone as a blocking agent of tannins, did not change significantly the EC50 for branches extract, but significantly changed for leaves extract. Quercetin and gallocatechin EC50 values were in the range 0.03–0.05 mg/mL and epicatechin showed 100% inhibition of the egg hatching process at 0.004mg/mL. Copyright © 2021 Informa UK Limited, trading as Taylor & Francis Group  aEgg hatch inhibition assay  aMass spectrometry  aMaytenus ilicifolia  aPhenolic compounds  aRuminants anthelmintic activity1 aESCOBAL, M.1 aDE SOUZA, G.1 aMEDEROS, A.  tNatural Product Research, 2021. Doi: https://doi.org/10.1080/14786419.2021.1948844