04105naa a2200445 a 450000100080000000500110000800800410001902400350006010000150009524501540011026000090026450005920027352021430086565000110300865000150301965300130303465300260304765300290307365300300310265300100313265300290314265300160317165300490318765300310323665300320326765300180329970000170331770000170333470000150335170000180336670000210338470000170340570000190342270000160344170000170345770000150347470000140348970000180350377301380352110619872022-09-02       2021    bl uuuu     u00u1 u    #d7 a10.1016/j.ram.2021.02.0052DOI1 aDORSCH, M.  aPlacentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay.h[electronic resource]  c2021  aArticle history: Received 22 July 2020/ Accepted 28 February 2021. Corresponding author:E-mail address: fgiannitti@inia.org.uy (F. Giannitti). This work was financially supported by grants N-15156 PL 150 00 and PL 27 N-23398 of the Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay).Please cite this article in press as: M.A. Dorsch, M.L. Casaux, L. Calleros et al., Placentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay, Revista Argentina de Microbiología,https://doi.org/10.1016/j.ram.2021.02.005.  aResumen:  Campylobacter fetus fetus (Cff) es una importante causa de abortos en ovinos y un patógeno oportunista en humanos. La información sobre Cff como abortifaciente en ovinos en Sudamérica es limitada. Describimos un caso de aborto causado por una cepa de Cff multirresistente a antibióticos en una oveja en Uruguay. En agosto de 2017, 3/57 ovejas prenadas ?(5,3%) abortaron en una semana. El examen histopatológico de la placenta de una de ellas reveló placentitis neutrofílica fibrinonecrosante severa, vasculitis y trombosis. Cff fue aislado en microaerobiosis en agar Skirrow, y confirmado mediante amplificación del ADNr 16S por PCR seguida de secuenciación, y por PCR punto final y qPCR. Las pruebas de sensibilidad antimicrobiana revelaron resistencia a tetraciclinas, ácido nalidíxico, telitromicina y clindamicina. No se detectaron otros abortifacientes. Son necesarios más estudios para determinar la distribución geográfica, ecología, epidemiología, el impacto económico y la resistencia antimicrobiana de Cff en majadas ovinas de Uruguay.  Abstract Campylobacter fetus fetus (Cff) is a major infectious cause of abortion in sheep worldwide, and an opportunistic human pathogen. Information on Cff as an ovine abortifacientin South America is limited. We describe a case of abortion caused by a multidrug resistant strain of Cff in a sheep in Uruguay. In August 2017, 3/57 pregnant ewes (5.3%) aborted whithin one week. Histopathologic examination of the placenta of an aborted ewe revealed severe neutrophilic and fibrinonecrotizing placentitis with vasculitis and thrombosis of the chorionic arterioles. Cff was isolated on microaerobic culture in Skirrow agar, and further confirmed by 16S rDNA PCR amplification and sequencing, and endpoint and real time PCR assays. Antimicrobial sensitivity testing revealed resistance to tetracyclines, nalidixic acid, telithromycin and clindamycin. Other abortifacients were not detected. Further studies are necessary to determine the geographic distribution, ecology, epidemiology, economic impact, and antimicrobial resistance of Cff in sheep flocks in Uruguay.  aABORTO  aSUDAMERICA  aABORTION  aANTIBIOTIC RESISTANCE  aCAMPILOBACTERIOSIS OVINA  aCAMPYLOBACTER FETUS SUBSP  aFETUS  aOVINE CAMPYLOBACTERIOSIS  aPLACENTITIS  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL  aPLATAFORMA DE SALUD ANIMAL  aRESISTENCIA A ANTIBIÓTICOS  aSOUTH AMERICA1 aCASAUX, M.L.1 aCALLEROS, L.1 aARÁOZ, V.1 aCAFFARENA, D.1 aMONESIGLIO, M.C.1 aBARCELLOS, M1 aSILVEIRA, C.S.1 aPERDOMO, T.1 aBANCHERO, G.1 aUZAL, F.A.1 aFRAGA, M.1 aGIANNITTI, F.  tRevista Argentina de Microbiología, 2022, volume 54, issue 1, pages 25-30. Open Acces. Doi:https://doi.org/10.1016/j.ram.2021.02.005