02929naa a2200301 a 450000100080000000500110000800800410001902200140006002400300007410000140010424501840011826000090030250006020031152013450091365000110225865300080226965300200227765300090229765300200230665300290232670000120235570000210236770000170238870000190240570000180242470000130244277301720245510616882022-02-24       2021    bl uuuu     u00u1 u    #d  a1661-65967 a10.3390/ijms220208042DOI1 aSALVO, M.  aTranscriptional analysis of C-repeat binding factors in fruit of citrus species with differential sensitivity to chilling injury during postharvest storage.h[electronic resource]  c2021  aArticle history: Received 9 December 2020; Revised 5 January 2021; Accepted 12 January 2021; Published 15 January 2021. This work was supported by a research grant from the National Agency of Research and Innovation (ANII-Uruguay) by the code FCE_3_2016_1_126714 and by a research grant RTI2018? 095131-B-I00 of the Ministry of Science and Innovation (Spanish Government). F. Rey is the recipient of a predoctoral scholarship (POS_EXT_2016_1_133720) from ANII (Uruguay). Supplementary material. This article belongs to the Special Issue Gene Transcriptional Regulation in Crops during Postharvest.  aABSTRACT. Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of Citrus CBFs in fruit response to cold, an in silico study was performed, revealing three genes (CBF1, CBF2, and CBF3) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1?5 d). Interestingly, CBF1 was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of CBF1 expression with cold tolerance in Citrus cultivars (except for mandarins), whereas the expression of CBF2 or CBF3 genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive Citrus, indicating that CBF1 participates in the natural tolerance to CI.  aCITRUS  aCBF  aChilling injury  aDREB  aGene expression  aPLATAFORMA AGROALIMENTOS1 aREY, F.1 aARRUABARRENA, A.1 aGAMBETTA, G.1 aRODRIGO, M. J.1 aZACARÍAS, L.1 aLADO, J.  tInternational Journal of Molecular Sciences, 2 January 2021, Volume 22, Issue 2, Article number 804, Pages 1-20. OPEN ACCESS. Doi: https://doi.org/10.3390/ijms22020804