02830naa a2200349 a 450000100080000000500110000800800410001902400380006010000170009824501150011526000090023050003150023952015000055465000140205465000120206865300210208065300110210165300130211265300140212565300310213970000180217070000170218870000150220570000170222070000170223770000180225470000200227270000180229270000170231070000150232777301380234210615252020-11-27       2020    bl uuuu     u00u1 u    #d7 a10.1016/j.meegid.2020.1046132DOI1 aCASTELLS, M.  aDetection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay.h[electronic resource]  c2020  aArticle history: Received 1 August 2020 / Revised 27 October 2020 / Accepted 28 October 2020 / Available online 4 November 2020./ Corresponding authors at.: Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera, 1350,50000 Salto, Uruguay.  aAbstracts. Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ?2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.  aGANADERIA  aURUGUAY  aBOVINE NOROVIRUS  aCATTLE  aDIARRHEA  aGENOTYPES  aPLATAFORMA DE SALUD ANIMAL1 aCAFFARENA, D.1 aCASAUX, M.L.1 aSCHILD, C.1 aCASTELLS, F.1 aCASTELLS, D.1 aVICTORIA , M.1 aRIET-CORREA, F.1 aGIANNITTI, F.1 aPARREÑO, V.1 aCOLINA, R.  tInfection, Genetics and Evolution, December 2020, Volume 86, Article number 104613. Doi: https://doi.org/10.1016/j.meegid.2020.104613