02407naa a2200277 a 450000100080000000500110000800800410001902400290006010000190008924501720010826000090028052015210028965300110181065300120182165300210183365300140185465300220186865300280189065300170191865300150193570000140195070000170196470000150198170000140199677301190201010613502020-09-28       2020    bl uuuu     u00u1 u    #d7 a10.3920/BM2019.01672DOI1 aFERNÁNDEZ ,S.  aEffect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves.h[electronic resource]  c2020  aAbstract: Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves' health. The objective of this study was to evaluate the effect of potential probiotic lactobacilli on new-born dairy calves' growth, diarrhoea incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels in plasma and faeces, and pathogen abundance in faeces. Two in vivo assays were conducted at the same farm in two annual calving seasons. Treated calves received one daily dose of the selected lactobacilli (Lactobacillus reuteri TP1.3B or Lactobacillus johnsonii TP1.6) for 10 consecutive days. A faecal score was recorded daily, average daily gain (ADG) was calculated, and blood and faeces samples were collected. Pathogen abundance was analysed by absolute qPCR in faeces using primers directed at Salmonella enterica, rotavirus, coronavirus, Cryptosporidium parvum and three Escherichia coli virulence genes (eae, clpG and Stx1). The faecal score was positively affected by the administration of both lactobacilli strains, and diarrhoea incidence was significantly lower in treated calves. No differences were found regarding ADG, cytokine expression, IgA levels and pathogen abundance. Our findings showed that oral administration of these strains could improve gastrointestinal health, but results could vary depending on the calving season, which may be related to pathogen seasonality and other environmental effects.  aCALVES  aDIARREA  aDIARREA NEONATAL  aDIARRHOEA  aLACTOBACILLUS SPP  aPLATAFORMA SALUD ANIMAL  aPROBIÓTICOS  aPROBIOTICS1 aFRAGA, M.1 aCASTELLS, M.1 aCOLINA, R.1 aZUNINO,P.  tBeneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167