03064naa a2200385 a 450000100080000000500110000800800410001902400380006010000160009824501600011426000090027450004730028352014620075665000100221865300100222865300080223865300080224665300170225465300100227165300120228170000190229370000190231270000150233170000190234670000200236570000210238570000180240670000170242470000140244170000190245570000160247470000210249070000160251177301510252710612092020-07-08       2020    bl uuuu     u00u1 u    #d7 a10.1016/j.meegid.2020.1044212DOI1 aWEBER, M.N.  aVirome of crab-eating (Cerdocyon thous) and pampas foxes (Lycalopex gymnocercus) from southern Brazil and Uruguay. (Research Paper).h[electronic resource]  c2020  aArticle history: Received 21 February 2020, Revised 8 June 2020, Accepted 11 June 2020, Available online 21 June 2020. Acknowledgements: The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Finance Code 001, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Reitoria de Pesquisa (PROPESQ-UFRGS) for supporting this study.  aABSTRACT: Crab-eating (Cerdocyon thous) and Pampas foxes (Lycalopex gymnocercus) are wild canids distributed in South America. Domestic dogs (Canis lupus familiaris) and wild canids may share viral pathogens, including rabies virus (RABV), canine distemper virus (CDV), and canine parvovirus 2 (CPV-2). To characterize the virome of these wild canid species, the present work evaluated the spleen and mesenteric lymph node virome of 17 crab-eating and five Pampas foxes using high-throughput sequencing (HTS). Organ samples were pooled and sequenced using an Illumina MiSeq platform. Additional PCR analyses were performed to identify the frequencies and host origin for each virus detected by HTS. Sequences more closely related to the Paramyxoviridae, Parvoviridae and Anelloviridae families were detected, as well as circular Rep-encoding single-stranded (CRESS) DNA viruses. CDV was found only in crab-eating foxes, whereas CPV-2 was found in both canid species; both viruses were closely related to sequences reported in domestic dogs from southern Brazil. Moreover, the present work reported the detection of canine bocavirus (CBoV) strains that were genetically divergent from CBoV-1 and 2 lineages. Finally, we also characterized CRESS DNA viruses and anelloviruses with marked diversity. The results of this study contribute to the body of knowledge regarding wild canid viruses that can potentially be shared with domestic canids or other species.  aVIRUS  aCANID  aFOX  aHTS  aMETAGENOMICS  aVIRUS  aWILDIFE1 aMOSENA, A.C.S.1 aDA SILVA, M.S.1 aCANOVA, R.1 aDE LORENZO, C.1 aOLEGÁRIO, J.C.1 aBUDASZEWSKI, R.F1 aBAUMBACH, L.F1 aSOARES, J.F.1 aSONNE, L.1 aVARELA, A.P.M.1 aMAYER, F.Q.1 aOLIVEIRA, L.G.S.1 aCANAL, C.W.  tInfection, Genetics and Evolution, November 2020, Volume 85, Article number 104421. OPEN ACCESS. Doi: https://doi.org/10.1016/j.meegid.2020.104421