04598naa a2200325 a 450000100080000000500110000800800410001902200140006002400340007410000210010824501120012926000090024150000990025052035400034965300230388965300190391265300230393165300120395465300180396665300130398465300280399770000160402570000160404170000260405770000180408370000220410170000200412370000190414377301100416210607212020-01-30       2019    bl uuuu     u00u1 u    #d  a1678-03457 a10.22456/1679-9216.918772DOI1 aDE SOUTO, E.P.F.  aMastite por Pythium insidiosum em éguas. [Mastitis by Pythium insidiosum in Mares.]h[electronic resource]  c2019  aArticle history: Received: 22 January 2019 / Accepted: 27 April 2019 / Published: 19 May 2019.  aABSTRACT. Background: Pythiosis is a chronic inflammatory disease caused by the oomycete Pythium insidiosum. This disease has been reported in several species, but most cases occur in horses. In this species, the cutaneous form is more prevalent, with occasional involvement of adjacent lymph nodes and bone tissue. The involvement of the mammary glands was not properly characterized. Therefore, the aim of this study is to describe cases of pythiosis with the involvement of the mammary glands in mares. Case: A retrospective study of all biopsy samples and necropsy examinations of horses, performed at the Animal Pathology Laboratory of the Federal University of Campina Grande from January 2000 to December 2018, was carried out. During the study period, 142 cases of cutaneous pythiosis were identified. Of these, in three the lesions extended to the mammary glands. Mares with 3-, 7- and 12-year-old, mongrel, from the backlands of the Paraiba were affected. On physical examination, the mares presented swollen mammary glands and multifocal wounds, which discharge serosanguinolent secretion. Clinical evolution ranged from 15 to 30 days. The animals had been medicated, but the wounds did not heal and mastectomies were performed. Macroscopically, the mammary glands were enlarged and with multifocal areas of ulceration measuring 1 to 3.5 cm in diameter. At the cut surface, they exhibited fistulous tracts containing kunkers. In the histopathological evaluation, was observed diffuse and marked inflammatory infiltrate of eosinophils extending through the deep dermis and interlobular mammary stroma; and multifocal areas, sometimes focally extensive, of eosinophilic necrosis with intralesional negative images of hyphae. In histological sections stained by periodic acid-Schiff, the walls of the hyphae were weakly stained in blue; but when impregnated by Grocott methenamine silver, they were strongly marked in black and were characterized by semi-parallel walls, rare septations and branching, and a diameter varying from 3-8 ?m. At immunohistochemistry, the hyphae showed strong cytoplasmic and wall immunostaining for the anti-P. insidiosum antibody. Discussion: The diagnosis of mastitis by Pythium insidiosum was established based on the epidemiological, clinical, anatomopathological and immunohistochemical findings. Lesions in horses are more frequently located at the extremities of the limbs and the ventral portion of the thoracoabdominal wall. In these cases, it is likely that the lesions have started on the skin adjacent to the udder and have extended to the mammary parenchyma. The intralesional structures known as kunkers have been described in horses, mules, donkeys, and camels, being absent in the other animal species affected. The kunkers are always present in equine pythiosis and allow the establishment of an accurate presumptive diagnosis. Clinically important findings include wounds that discharge serosanguineous secretions and do not heal through conventional treatments. Histological lesions characterized by areas of eosinophilic necrosis with intralesional hyphae, which are weakly stained by PAS and strongly impregnated by GMS, are highly suggestive of pythiosis. Pythiosis is relatively frequent in horses in the backlands of Paraiba, predominantly in the cutaneous form. The involvement of the mammary glands is an uncommon presentation that should be included as a differential diagnosis of mastitis in mares. © 2019 Universidade Federal do Rio Grande do Sul. All rights reserved.  aDoença de equinos  aEquine disease  aGlândula mamária  aKunkers  aMammary gland  aOomycete  aPLATAFORMA SALUD ANIMAL1 aMAIA, L.Â.1 aASSIS, D.M.1 aDE MIRANDA NETO, E.G.1 aKOMMERS, G.D.1 aDE GALIZA, G.J.N.1 aRIET-CORREA, F.1 aDANTAS, A.F.M.  tActa Scientiae Veterinariae, 2019, Volume 47 (Suppl.1), Article number 387. Doi: 10.22456/1679-9216.91877