02438naa a2200277 a 450000100080000000500110000800800410001902400390006010000150009924501140011426000090022850002710023752013690050865300100187765300190188765300200190665300130192665300140193965300120195370000200196570000140198570000160199970000150201570000140203077301160204410605982020-06-01       2020    bl uuuu     u00u1 u    #d7 a10.1080/00275514.2019.16948272DOI1 aOBERTI, H.  aDiversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes.h[electronic resource]  c2020  aArticle history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19).  aClaviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before.  aERGOT  aFINGERPRINTING  aHOST SPECIATION  aPASPALUM  aPHYLOGENY  aSTAGGER1 aDALLA RIZZA, M.1 aREYNO, R.1 aMURCHIO, S.1 aALTIER, N.1 aABREO, E.  tMycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827