02595naa a2200313 a 450000100080000000500110000800800410001902400360006010000130009624501570010926000090026650000750027552015330035065000120188365300320189565300290192770000240195670000190198070000180199970000170201770000150203470000170204970000210206670000160208770000170210370000200212070000150214077301260215510605132020-04-20       2020    bl uuuu     u00u1 u    #d7 a10.1007/s00705-019-04446-z2DOI1 aMAYA, L.  aAn extensive field study reveals the circulation of new genetic variants of subtype 1a of bovine viral diarrhea virus in Uruguay.h[electronic resource]  c2020  aArticle history:Received: 22 April 2019 / Accepted: 21 September 2019.  aAbstract: Bovine viral diarrhea virus (BVDV) is a major pathogen worldwide, causing signifcant economic losses to the livestock sector. In Uruguay, BVDV seroprevalence at the farm level is >80%. In this work, 2546 serum, blood or tissue samples collected from animals suspected of being afected by BVD between 2015 and 2017 were analyzed by reverse transcription PCR and sequencing. Analysis of the BVDV genomic regions 5?UTR/Npro, Npro and E2 revealed that BVDV-1a, 1i and 2b circulate in the country, with BVDV-1a being the most prevalent subtype. Population dynamics studies revealed that BVDV-1a has been circulating in our herds since ~1990. This subtype began to spread and evolve, accumulating point mutations at a rate of 3.48 × 10?3 substitutions/site/year, acquiring specifc genetic characteristics that gave rise to two local genetic lineages of BVDV-1a. These lineages are divergent from those circulating worldwide, as well as the vaccine strain currently used in Uruguay. The most notable diferences between feld and vaccine strains were found in the E2 glycoprotein, suggesting that the amino acid substitutions could result in failure of cross-protection/neutralization after vaccination. This is the frst study that compares Uruguayan BVDV feld and vaccine strains with other BVDV strains from throughout the world. The results obtained in this study will be very useful for developing a suitable immunization program for BVDV in Uruguay by identifying local feld strains as candidates for vaccine development.  aDIARREA  aBOVINE VIRAL DIARRHEA (BVD)  aPLATAFORMA SALUD ANINMAL1 aMACÍAS-RIOSECO, M.1 aSILVEIRA, C.S.1 aGIANNITTI, F.1 aCASTELLS, M.1 aRIVERO, R.1 aCRISTINA, J.1 aGIANNEECHINI, E.1 aPUENTES, R.1 aFLORES, E.F.1 aRIET-CORREA, F.1 aCOLINA, R.  tArchives of Virology, 1 January 2020, Volume 165, Issue 1, Pages 145-156. Doi: https://doi.org/10.1007/s00705-019-04446-z