02386naa a2200289 a 450000100080000000500110000800800410001902400320006010000180009224502230011026000090033350000720034252013910041465000140180565000190181965300150183865300120185365300230186565300270188865300180191570000170193370000160195070000120196670000160197870000190199477300830201310603742019-10-31       2019    bl uuuu     u00u1 u    #d7 a10.3168/jds.2019-166292DOI1 aCAPELESSO, A.  aReducing milking frequency in early lactation improved the energy status but reduced milk yield during the whole lactation of primiparous Holstein cows consuming a total mixed ration and pasture.h[electronic resource]  c2019  aArticle history: Received March 15, 2019. / Accepted June 21, 2019.  aAbstract: To investigate the immediate and long-term performance effects of milking frequency during early lactation of primiparous dairy cows consuming a total mixed ration and pasture, 20 Holstein cows were assigned in a randomized block design to either once-daily (1×) or twice-daily (2×) milking during the first 8 wk of lactation (treatment period). After the treatment period, all cows were milked 2× until wk 43 of lactation. Cows were fed a total mixed ration (approximately 15 kg of DM/cow per day) and allowed to graze an oat pasture (Avena sativa). Dry matter intake was 19.1 kg of DM/cow per day on average and was not affected by treatments. Milk yield was 40% lower in cows milked 1× during the treatment period, and a carryover effect existed until wk 21 of lactation, resulting in a final reduction of 15% of milk yield in the whole lactation. Milk lactose concentration decreased, whereas fat and protein concentrations increased for cows milked 1×. Mobilization of energy reserves during the treatment period occurred in both groups, but cows milked 1× showed greater body condition score and greater backfat thickness. In conclusion, milking 1× during the first 8 wk of lactation resulted in immediate and carryover negative effects on milk and milk solid yield without affecting feed intake, resulting in the improved energy status of primiparous dairy cows.  aLECHERÍA  aVACAS LECHERAS  aDAIRY COWS  aGRAZING  aONCE-DAILY MILKING  aORDEÑE UNA VEZ AL DIA  aVACA HOLSTEIN1 aKOZLOSKI, G.1 aMENDOZA, A.1 aPLA, M.1 aREPETTO, J.1 aCAJARVILLE, C.  tJournal of Dairy Science, October 2019, Volume 102, Issue 10, Pages 8919-8930.