02739naa a2200313 a 450000100080000000500110000800800410001902200140006002400360007410000300011024501280014026000090026850003520027752015070062965300240213665300180216065300080217865300080218665300240219470000270221870000180224570000140226370000140227770000210229170000140231270000150232670000140234177300700235510599422019-08-26       2019    bl uuuu     u00u1 u    #d  a1983-20527 a10.1007/s40858-019-00288-x2DOI1 aHERNÁNDEZ-RODRÍGUEZ, L.  aDiversity of Uruguayan citrus tristeza virus populations segregated after single aphid transmission.h[electronic resource]  c2019  aArticle history: Received 16 November 2018 / Accepted 10 April 2019 / First Online 06 May 2019 // Published 15 August 2019. Acknowledgments: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay (project CT-06), through the National Program of Citrus Research and the National Citrus Sanitation Program.  aABSTRACT. Prevalence of citrus tristeza virus (CTV) isolates causing severe stem pitting symptoms with a high diverse genetic composition represents a threat for citrus crops in Uruguay. This work aimed to characterize viral components of CTV populations segregated by single aphid (Toxoptera citricida) transmission (SAT). Thirty-nine SAT-derived sub-isolates were obtained from six CTV field isolates. Only eight were MCA13 non-reactive, and six of them, together with other three MCA13 reactive sub-isolates, were considered mild by the intensity of their reaction in ?Mexican? lime, ?Madam Vinous? sweet orange, ?Duncan? grapefruit and sour orange indicator plants. Fourteen sub-isolates, including two MCA13 non-reactive, were considered moderate, and the remaining sub-isolates were all MCA13 reactive and with severe reactions in the indicator plants. Sequence comparisons of the p20, p23 and p25 genes showed a high inter (among all isolates and their derived sub-isolates)- and intra (among each isolate and its derived sub-isolates)-specific evolutionary diversity, with the presence of the CTV strains VT, T3, RB, and NC in mixtures of their complex populations. Together, our data revealed the heterogeneity of the Uruguayan CTV populations in these CTV field isolates. The results provide additional biological and molecular information on the complex CTV populations in Uruguay and must be considered for improving the CTV management program. © Sociedade Brasileira de Fitopatologia 2019  aBiological indexing  aClosterovirus  aCTV  aSAT  aToxoptera citricida1 aBENÍTEZ-GALEANO, M.J.1 aBERTALMIO, A.1 aRUBIO, L.1 aRIVAS, F.1 aARRUABARRENA, A.1 aROLON, R.1 aCOLINA, R.1 aMAESO, D.  tTropical Plant Pathology, 2019, volume 44, Issue 4, pages 352-362