02505naa a2200241 a 450000100080000000500110000800800410001902200140006002400360007410000140011024501680012426000090029250003210030152013830062265300260200565300150203165300110204665300130205765300240207065300140209470000150210877301400212310597972020-04-20       2019    bl uuuu     u00u1 u    #d  a2045-23227 a10.1038/s41598-018-37118-02DOI1 aABREO, E.  aPangenome of Serratia marcescens strains from nosocomial and environmental origins reveals different populations and the links between them.h[electronic resource]  c2019  aArticle history: Received: 25 July 2018 // Accepted: 30 November 2018 // Published online: 10 January 2019.  Author Contributions: Eduardo Abreo and Nora Altier designed the study, E.A. performed the genomic analysis and wrote the manuscript, E.A. and N.A. analyzed the results. Both authors reviewed the manuscript.  aABSTRACT. Serratia marcescens is a Gram-negative bacterial species that can be found in a wide range of environments like soil, water and plant surfaces, while it is also known as an opportunistic human pathogen in hospitals and as a plant growth promoting bacteria (PGPR) in crops. We have used a pangenome-based approach, based on publicly available genomes, to apply whole genome multilocus sequence type schemes to assess whether there is an association between source and genotype, aiming at differentiating between isolates from nosocomial sources and the environment, and between strains reported as PGPR from other environmental strains. Most genomes from a nosocomial setting and environmental origin could be assigned to the proposed nosocomial or environmental MLSTs, which is indicative of an association between source and genotype. The fact that a few genomes from a nosocomial source showed an environmental MLST suggests that a minority of nosocomial strains have recently derived from the environment. PGPR strains were assigned to different environmental types and clades but only one clade comprised strains accumulating a low number of known virulence and antibiotic resistance determinants and was exclusively from environmental sources. This clade is envisaged as a group of promissory MLSTs for selecting prospective PGPR strains.  © 2019, The Author(s).  aANTIBIOTIC RESISTANCE  aCLADISTICS  aGENOME  aGENOTYPE  aSERRATIA MARCESCENS  aVIRULENCE1 aALTIER, N.  tScientific Reports, 1 December 2019, Volume 9, Issue 1, Article number 46. OPEN ACCESS. Doi: https://doi.org/10.1038/s41598-018-37118-0