03375naa a2200325 a 450000100080000000500110000800800410001902400320006010000180009224501560011026000090026650000730027552022700034865000180261865000190263665300240265565300400267965300200271965300120273965300590275165300170281065300250282765300290285265300230288170000160290470000160292070000160293670000190295277300780297110595912019-10-30       2019    bl uuuu     u00u1 u    #d7 a10.3168/jds.2018-153892DOI1 aPASTORINI, M.  aProductive performance and digestive response of dairy cows fed different diets combining a total mixed ration and fresh forage.h[electronic resource]  c2019  aArticle history: Received July 16, 2018. //Accepted January 2, 2019.  aABSTRACT: The purpose of this experiment was to determine the effects of feeding increasing levels of fresh forage (FF) as a proportion of total dry matter intake (DMI) on nutrient intake, rumen digestion, nutrient utilization, and productive performance of total mixed ration (TMR)-fed cows. Twelve dairy cows (90 ± 22 d in milk, 523 ± 88 kg of body weight, 7,908 ± 719 kg of milk production in the previous lactation) were housed in individual tiestalls and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. Treatments were 100% TMR (T100), 75% TMR plus 25% FF (T75), and 50% TMR plus 50% FF (T50). The experiment lasted 60 d, divided into 3 periods of 20 d each; the first 12 d of each period were used for diet adaptation and the last 8 d for data collection. The TMR (18.1% crude protein, 24.6% acid detergent fiber) and FF (Lolium multiflorum; 15.1% crude protein, 24.1% acid detergent fiber) were prepared and cut daily and offered to each cow individually. The highest DMI was reached in T100 and T75, which was reflected in greater intake of the different nutrients than T50. No differences were detected in the apparent total digestibility of the nutrients, mean ruminal pH, and total volatile fatty acid concentrations among treatments. Cows in T50 resulted in the lowest ruminal N-NH3 concentration and the lowest microbial N flow to the duodenum. Milk yield was 8.5% higher from cows in T100 and T75 compared with T50, but we observed no differences for milk fat or milk protein yield among treatments. Milk fat of cows fed T50 had 8% more unsaturated fatty acids (FA) than that of cows fed T100, mostly because of a higher content of monounsaturated FA. Additionally, cows in T50 had a higher concentration of linoleic acid, vaccenic acid, and rumenic acid than T100. Meanwhile, the concentration of linoleic acid and vaccenic acid in cows fed T75 was higher than T100. The milk fat of the cows fed T50 and T75 had a lower n-6:n-3 ratio than T100. We concluded that including up to 29% of FF in the total DMI in combination with a TMR did not affect the intake or digestion of nutrients or the productive response in dairy cows and resulted in a higher concentration of desirable FA from a consumer's perspective.  aALIMENTACIÓN  aVACAS LECHERAS  aFORRAJE FRESCO (FF)  aINGESTA TOTAL DE MATERIA SECA (DMI)  aMILK PRODUCTION  aPASTURE  aRENDIMIENTO PRODUCTIVO DE LA RACIÓN MIXTA TOTAL (TMR)  aRUMENIC ACID  aRUMINAL FERMENTATION  aSISTEMA DE ALIMENTACIÓN  aTOTAL MIXED RATION1 aPOMIÉS, N.1 aREPETTO, J.1 aMENDOZA, A.1 aCAJARVILLE, C.  tJournal of Dairy Science, May 2019, Volume 102, Issue 5, Pages 4118-4130.