02838naa a2200301 a 450000100080000000500110000800800410001902200390006002400350009910000240013424501620015826000090032050001090032952017840043865000110222265300140223365300210224765300200226865300240228870000300231270000210234270000160236370000240237970000170240370000260242070000190244677300710246510593442018-12-05       2018    bl uuuu     u00u1 u    #d  a0014-2336 // Online ISSN 1573-50607 a10.1007/s10681-018-2253-92DOI1 aGONZÁLEZ-ARCOS, M.  aIdentification of genetic sources with attenuated Tomato chlorosis virus-induced symptoms in Solanum (section Lycopersicon) germplasm.h[electronic resource]  c2018  aArticle history: Received 26 March 2018 // Accepted 04 September 2018 // First Online 22 September 2018.  aABSTRACT. The whitefly-transmitted Tomato chlorosis virus (ToCV) (genus Crinivirus) is associated with yield and quality losses in field and greenhouse-grown tomatoes (Solanum lycopersicum) in South America. Therefore, the search for sources of ToCV resistance/tolerance is a major breeding priority for this region. A germplasm of 33 Solanum (Lycopersicon) accessions (comprising cultivated and wild species) was evaluated for ToCV reaction in multi-year assays conducted under natural and experimental whitefly vector exposure in Uruguay and Brazil. Reaction to ToCV was assessed employing a symptom severity scale and systemic virus infection was evaluated via RT-PCR and/or molecular hybridization assays. A subgroup of accessions was also evaluated for whitefly reaction in two free-choice bioassays carried out in Uruguay (with Trialeurodes vaporariorum) and Brazil (with Bemisia tabaci Middle-East-Asia-Minor1?MEAM1 = biotype B). The most stable sources of ToCV tolerance were identified in Solanum habrochaites PI 127827 (mild symptoms and low viral titers) and S. lycopersicum ?LT05? (mild symptoms but with high viral titers). These two accessions were efficiently colonized by both whitefly species, thus excluding the potential involvement of vector-resistance mechanisms. Other promising breeding sources were Solanum peruvianum (sensu lato) ?CGO 6711? (mild symptoms and low virus titers), Solanum chilense LA1967 (mild symptoms, but with high levels of B. tabaci MEAM1 oviposition) and Solanum pennellii LA0716 (intermediate symptoms and low level of B. tabaci MEAM1 oviposition). Additional studies are necessary to elucidate the genetic basis of the tolerance/resistance identified in this set of Solanum (Lycopersicon) accessions.  © 2018, Springer Nature B.V.  aTOMATE  aTOLERANCE  aVIRUS RESISTANCE  aVIRUS SCREENING  aWHITEFLY RESISTANCE1 aDE NORONHA FONSECA, M. E.1 aARRUABARRENA, A.1 aLIMA, M. F.1 aMICHEREFF-FILHO, M.1 aMORIONES, E.1 aFERNÁNDEZ-MUÑOZ, R.1 aBOITEUX, L. S.  tEuphytica, 1 October 2018, vol. 214, Issue 10, Article number 178.