01808naa a2200277 a 450000100080000000500110000800800410001902200140006002400380007410000160011224501780012826000090030650003080031552005920062365000140121565300110122965300360124065300260127665300280130265300250133065300100135570000170136570000170138270000160139977301150141510572182020-06-02       2017    bl uuuu     u00u1 u    #d  a1874-39007 a10.1016/j.phytol.2017.04.0202DOI1 aWALLACE, F.  aAnalysis of an immunoadjuvant saponin fraction from Quillaja brasiliensis leaves by electrospray ionization ion trap multiple-stage mass spectrometry.h[electronic resource]  c2017  aArticle history: Received: 16 November 2016 // Received in revised form: 15 February 2017 // Accepted 22 April 2017. Acknowledgments: Funding from the Instituto Nacional de Investigación Agropecuaria (award L4-FO-21-0-00) and the Programa de Desarrollo de las Ciencias Básicas is greatly acknowledged.  aAn immunoadjuvant saponin fraction from Quillaja brasiliensis leaves was investigated by direct infusion and liquid chromatography/electrospray ionization ion trap multiple-stage mass spectrometry in negative ion mode (DI-ESI-IT-MSn and LC-ESI-IT-MSn ). The aglycone and the sequence of the oligosaccharide residues at C-3 and C- 28 were characterized based on MS2 and MS3 experiments of the [Me-H]- ions. According to their [Me-H]- ions, characteristic product ions and retention times, 27 bidesmosidic saponins, bearing four types of triterpenic aglycones, were tentatively identified.  aSAPONINAS  aESI-MS  aIMMUNOADJUVANT SAPONIN FRACTION  aQUILLAJA BRASILIENSIS  aQUILLAJA LEAVES EXTRACT  aTRITERPENIC SAPONINS  aUHPLC1 aBENNADJI, Z.1 aFERREIRA, F.1 aOLIVARO, C.  tPhytochemistry Letters, Volume 20, June 2017, Pages 228-233. DOI: https://doi.org/10.1016/j.phytol.2017.04.020