02687naa a2200301 a 450000100080000000500110000800800410001902200140006002400280007410000200010224501260012226000090024850000440025752017980030165000210209965000090212065000140212965000120214365000220215565300270217765300210220465300190222565300250224470000150226970000150228470000140229977300720231310570112017-04-11       2006    bl uuuu     u00u1 u    #d  a1099-209X7 a10.1007/BF028715902DOI1 aDALLA RIZZA, M.  aDetection of PVY extreme resistance genes in potato germplasm from the Uruguayan breeding program.h[electronic resource]  c2006  aAccepted for publication 14 March 2006.  aABSTRACT.  Breeding goals in potato programs encompass several important commercial traits such as market aspects (skin color, eye depth, shape), category use (fresh or processed), yield, crop duration, and pest and disease resistance. In light of medium- and long-term changes in trait requirements, breeders rely on a range of wild and commercial germplasm in their efforts to reach the multiple objectives that determine the success of a new variety. Readily selectable markers for the recognition of multiple introgressed traits in breeding populations would therefore be practical high-value tools. In subtropical countries, warm climate and the continuous cropping of potato promote high vector pressure and the spread of viruses, rendering seed certification schemes difficult. Potato virus Y (PVY) constitutes a constant problem in several developing countries and it was recently reported as a challenge for seed production in the U.S.A. and Spain, resulting in rejection of seed lots in certification programs. Here we report the simultaneous use of two molecular markers to identify genes for extreme resistance to PVY in the Uruguayan potato-breeding program germplasm. Simplicity and compatibility of the marker system (allele specificity) were emphasized in implementing a reliable and efficient procedure. Forty-four percent of the genotypes tested were shown to carry extreme resistance to PVY, in the majority of cases from Solanum tuberosum ssp. andigena (Ryadg). Furthermore, a single marker originating from Solanum stoloniferum (M45) could be used to recognize both Rysto and Ryadg. The molecular phenotype data corresponding to M45-Ry genotypes was in accordance with virus field exposure observations of resistance.  © Copyright 2009 Elsevier B.V., All rights reserved.  aFITOMEJORAMIENTO  aPAPA  aPOTYVIRUS  aSOLANUM  aSOLANUM TUBEROSUM  aALLELE-SPECIFIC MARKER  aPOTATO GERMPLASM  aPOTATO VIRUS Y  aSOLANUM STOLONIFERUM1 aVILARO, F.1 aTORRES, D.1 aMAESO, D.  tAmerican Journal of Potato Research, 2006gv. 83, no.4, p. 297-304.