02710naa a2200265 a 450000100080000000500110000800800410001902400430006010000170010324501470012026000090026750001080027652018250038465000110220965300250222065300260224565300100227165300110228165300230229270000190231570000150233470000190234970000140236877300620238210552442016-08-11       2016    bl uuuu     u00u1 u    #d7 a10.1016/j.postharvbio.2016.07.0022DOI1 aLOMBARDO, P.  aEffectiveness of essential oils for postharvest control of Phyllosticta citricarpa (citrus black spot) on citrus fruit.h[electronic resource]  c2016  aArticle history: Received 11 February 2016; Received in revised form 22 June 2016; Accepted 2 July 2016  aABSTRACT.  The in vitro and in vivo control of Phyllosticta citricarpa (citrus black spot, CBS) on citrus fruits was evaluated using 14 essential oils (EOs) extracted from Uruguayan native plants. In vitro, volatile components of Chenopodium ambrosioides completely inhibited fungal growth. The dilution agar test showed that fungus reproduction was completely inhibited by Conyza bonaerensis essential oil (EO). The other 12 EOs tested showed high inhibition after 10?15 d, presenting fungistatic effects. In vivo, assays were performed on fruit of lemon [Citrus limon (L.) Burm] and Valencia orange [Citrus sinensis (L.) Osbeck] in order to evaluate the effects of liquid and volatile EO phases on reproduction. Typical lesions of CBS without reproductive structures were exposed to Ch. ambrosioides and C. bonaerensis EOs for 20 d at 27 C and cycles of 16 h light and 8 h dark. The volatile components of Ch. ambrosioides were able to control 100% of P. citricarpa reproduction in orange and lemon fruit. The growth of other fungi such, as Colletotrichum gloeosporoides and Fusarium sp., was also inhibited. Nevertheless, the liquid phase was not effective to control the pathogen. In another test, carton boxes with 20 fruits, each presenting typical CBS symptoms, but without the presence of reproductive structures, were exposed to 0.01 and 0.05% of Ch. ambrosioides EO (w/v). The fruits were stored at 5 C for 20 d and then kept for 7 d at room temperature. A 40% reduction of reproduction was observed in citrus fruit exposed to 0.05% of EO. GC?MS analysis of EOs showed that Ch. ambrosioides EO was composed of 76% monoterpene compounds (49 and 27% of oxygenated and hydrocarbons, respectively), while C. bonaerensis EO presented 17% monoterpenes and 10% sequisterpenes.  @2016 Elsevier B.V. All rights reserved  aCITRUS  aBOTANICAL FUNGICIDES  aGUIGNARDIA CITRICARPA  aLEMON  aORANGE  aQUARANTINE DISEASE1 aGUIMARAENS, A.1 aFRANCO, J.1 aDELLACASSA, E.1 aPEREZ, E.  tPostharvest Biology and Technology, 2016gv. 121, p. 1-8.