02957naa a2200397 a 450000100080000000500110000800800410001902400360006010000180009624501050011426000090021950001070022852017530033565000270208865000090211565000230212465300230214765300220217065300160219265300220220870000190223070000180224970000160226770000150228370000170229870000180231570000210233370000150235470000180236970000160238770000170240370000170242070000140243770000210245177300870247210552412019-03-12       2015    bl uuuu     u00u1 u    #d7 a10.1016/j.ejbt.2015.06.0072DOI1 aROCHA, C.M.L.  aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.h[electronic resource]  c2015  aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015.  aABSTRACT.  Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919  markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.   © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve  aMARCADORES MOLECULARES  aSOJA  aVARIACION GENETICA  aASIAN SOYBEAN RUST  aGENETIC VARIATION  aGLYCINE MAX  aMOLECULAR MARKERS1 aVELLICCE, G.R.1 aGARCÍA, M.G.1 aPARDO, E.M.1 aRACEDO, J.1 aPERERA, M.F.1 aDE LUCÍA, A.1 aBONNECARRERE, M.1 aGERMAN, S.1 aMARCELINO, F.1 aLEDESMA, F.1 aREZNIKOV, S.1 aPLOPER, D.L.1 aWELIN, B.1 aCASTAGNARO, A.P.  tElectronic Journal of Biotechnology, 2015gv. 18, no. 6, p. 439-444.  OPEN ACCESS.