03126naa a2200265 a 450000100080000000500110000800800410001902400350006010000170009524501670011226000090027950001070028852022570039565000110265265000210266365000120268465300320269665300140272865300110274265300120275370000180276570000180278370000160280177300430281710539842021-06-24 2015 bl uuuu u00u1 u #d7 a10.1017/S17517311150016762DOI1 aCOTTLE, D.J. aEstimating daily methane production in individual cattle with irregular feed intake patterns from short-term methane emission measurements.h[electronic resource] c2015 aArticle history: Received 19 March 2015; Accepted 17 July 2015; First published online 24 August 2015. aSpot measurements of methane emission rate (n = 18 700) by 24 Angus steers fed mixed rations from GrowSafe feeders were made over 3- to 6-min periods by a reenFeed emission monitoring (GEM) unit. The data were analysed to estimate daily methane production (DMP; g/day) and derived methane yield (MY; g/kg dry matter intake (DMI)). A one-compartment dose model of spot emission rate v. time since the preceding meal was compared with the models of Wood (1967) and Dijkstra et al. (1997) and the average of spot measures. Fitted values for DMP were calculated from the area under the curves. Two methods of relating methane and feed intakes were then studied: the classical calculation of MY as DMP/DMI (kg/day); and a novel method of estimating DMP from time and size of preceding meals using either the data for only the two meals preceding a spot measurement, or all meals for 3 days prior. Two approaches were also used to estimate DMP from spot measurements: fitting of splines on a ?per-animal per-day? basis and an alternate approach of modelling DMP after each feed event by least squares (using Solver), summing (for each animal) the contributions from each feed event by best-fitting a one-compartment model. Time since the preceding meal was of limited value in estimating DMP. Even when the meal sizes and time intervals between a spot measurement and all feeding events in the previous 72 h were assessed, only 16.9% of the variance in spot emission rate measured by GEM was explained by this feeding information. While using the preceding meal alone gave a biased (underestimate) of DMP, allowing for a longer feed history removed this bias. A power analysis taking into account the sources of variation in DMP indicated that to obtain an estimate of DMP with a 95% confidence interval within 5% of the observed 64 days mean of spot measures would require 40 animals measured over 45 days (two spot measurements per day) or 30 animals measured over 55 days. These numbers suggest that spot measurements could be made in association with feed efficiency tests made over 70 days. Spot measurements of enteric emissions can be used to define DMP but the number of animals and samples are larger than are needed when day-long measures are made. aMETANO aMODELOS ANIMALES aVACUNOS aGASES DE EFECTO INVERNADERO aGREENFEED aMODELS aSPLINES1 aVELAZCO, J.I.1 aHEGARTY, R.S.1 aMAYER, D.G. tAnimal, 2015gv. 9 (12), p. 1949-1957.