02026naa a2200229 a 450000100080000000500110000800800410001902200440006002400450010410000140014924501240016326000090028750001670029652011450046365000110160865000080161965000100162765300380163765300310167565300280170677300620173410539432019-10-15       2015    bl uuuu     u00u1 u    #d  a0031-9465 (print) // 1593-2095 (online)7 a10.14601/Phytopathol_Mediterr-154412DOI1 aABREO, E.  aHaplotype analysis and genetic variability of Togninia minima from different geographic sources.h[electronic resource]  c2015  aRESEARCH PAPERS - 9TH SPECIAL ISSUE ON GRAPEVINE TRUNK DISEASES. Article history: Accepted for publication: March 21, 2015 / Published online: September 15, 2015.  aSUMMARY.  Togninia minima (anamorph Phaeoacremonium aleophilum) is one of the main fungi responsible for trunk diseases of grapevines and other woody hosts worldwide. Sequences of protein-coding genes of isolates from countries in different continents have been published, presenting a useful resource for examination of the diversity and spatial distribution of T. minima genotypes. Single nucleotide polymorphisms (SNPs) detected in public sequences of the actin and partial β-tubulin genes were used to assess the genetic variability and to determine haplotypes of isolates of this species from different sources. The Italian sample showed the greatest allele number and the largest number of haplotypes. Most haplotypes were present in more than one country, except for haplotype 11010 which was found only in Italy and 10111 found only in Canada. Haplotype 11111 was the most conspicuous and cosmopolitan, being present in six countries and on three host plant species. One observed polymorphism in the noncoding region of the β-tubulin gene could be targeted with allele-specific primers to detect this particular haplotype.  aPRUNUS  aSNP  aVITIS  aENFERMEDADES DEL TRONCO DE LA VID  aPHAEOACREMONIUM ALEOPHILUM  aPHAEOACREMONIUM MINIMUM  tPhytopatologia Mediterranea, 2015gv.54, no.2, p.335-344.